Development of HPLC Fingerprint Analysis of Traditional Diabetes Herbal Jamu Diabetes Plant Materials

2014 ◽  
Vol 68 (1) ◽  
Author(s):  
Gholamhossein Shams ◽  
Hassan Fahmi Ismail ◽  
Teh Liam Chee ◽  
M Abbas Ali ◽  
Fadzilah Adibah Abdul Majid ◽  
...  
Keyword(s):  
High Performance ◽  
Herbal Medicines ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprint ◽  
Plant Materials ◽  
Hplc Fingerprint ◽  
Herbal Formulation ◽  
Marker Compounds ◽  
Herbal Formulations

Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. In the present study, high-performance liquid chromatography (HPLC) detection was developed for chromatographic fingerprint analysis of the extract from grounded plant materials of Jamu Diabetes (JD), a well known herbal formulation in Malaysia. The bioactive markers rosmarinic acid, curcumin, cinnamaldehyde and andrographolide were identified from the methanol/water (80:20) extract of JD materials. The data presented in this study showed that the developed method is simple, sensitive and specific for simultaneous determination of the indicated marker compounds either qualitatively or quantitatively, and may be used as a fingerprint profile for the standardization of extractives or herbal medicines from herbal formulation JD materials.

scholarly journals Development of Quality Control Method for Glucofarmaka Antidiabetic Jamu by HPLC Fingerprint Analysis

2017 ◽  
Vol 17 (1) ◽  
pp. 79
Author(s):  
Hanifullah Habibie ◽  
Rudi Heryanto ◽  
Mohamad Rafi ◽  
Latifah Kosim Darusman
Keyword(s):  
Quality Control ◽  
High Performance ◽  
Control Method ◽  
Herbal Medicines ◽  
Analytical Performance ◽  
World Health ◽  
Fingerprint Analysis ◽  
Hplc Fingerprint ◽  
Quality Control Method ◽  
The World

Herbal medicines become increasingly popular all over the world for preventive and therapeutic purposes. Quality control of herbal medicines is important to make sure their safety and efficacy. Chromatographic fingerprinting has been accepted by the World Health Organization as one reliable strategy for quality control method in herbal medicines. In this study, high-performance liquid chromatography fingerprint analysis was developed as a quality control method for glucofarmaka antidiabetic jamu. The optimum fingerprint chromatogram were obtained using C18 as the stationary phase and linear gradient elution using 10–95% acetonitrile:water as the mobile phase within 60 minutes of elution and detection at 210 nm. About 20 peaks were detected and could be used as fingerprint of glucofarmaka jamu. To evaluate the analytical performance of the method, we determined the precision, reproducibility, and stability. The result of the analytical performance showed reliable results. The proposed method could be used as a quality control method for glucofarmaka antidiabetic jamu and also for its raw materials.

Fingerprint analysis and quantitative determination of 16 constituents of Antike capsule by high-performance liquid chromatography-photodiode array detection

2015 ◽  
Vol 7 (16) ◽  
pp. 6695-6704 ◽  
Author(s):  
Linrui Duan ◽  
Hua Li ◽  
Jiani Yuan ◽  
Hang Zhao ◽  
Xiaokai Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
High Performance ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprint ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Array Detection

A method was developed using high-performance liquid chromatography-photodiode array detection to obtain a chromatographic fingerprint of Antike capsule.

scholarly journals Determination of the bifenazate insecticide in apples and cucumbers by high performance liquid chromatography

2019 ◽  
Vol 55 (4) ◽  
pp. 422-428
Author(s):  
M. F. Zayats ◽  
S. M. Leschev
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Diode Array ◽  
The European Union ◽  
Optimal Conditions ◽  
Plant Materials ◽  
Ultraviolet Detection ◽  
Maximum Residue Level ◽  
Distribution Constants

Based on the distribution constants of biphenazate, obtained experimentally and also calculated from literature data on the solubility of biphenazate in water and organic solvents, as well as experimental data on the extraction of biphenazate from plant matrices by various extractants, we selected the optimal conditions for extracting biphenazate from apples and cucumbers. The conditions for the purification of the extracts were also selected. Acetonitrile in the presence of ammonium sulfate and hexane was used for extraction of the pesticide. Purification of extracts of plant materials was carried out by partitioning between hexane and water-acetonitrile mixture. The samples obtained after this treatment were pure enough to determine the residual amounts of biphenazate in them at the maximum residue level determined in Belarus and the countries of the European Union, or lower using widespread liquid chromatography with diode-array (ultraviolet) detection.

scholarly journals Improved Chromatographic Fingerprinting Combined with Multi-components Quantitative Analysis for Quality Evaluation of Penthorum chinense by UHPLC-DAD

2015 ◽  
Vol 10 (1) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Wangping Deng ◽  
Tongtong Xu ◽  
Min Yang ◽  
Yajun Cui ◽  
De-an Guo
Keyword(s):  
High Performance ◽  
Herbal Medicines ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Array Detector ◽  
Chromatographic Technique ◽  
Hplc Fingerprint ◽  
Solvent Flow Rate ◽  
Principle Components Analysis ◽  
Penthorum Chinense

A high performance liquid chromatographic (HPLC) fingerprint is commonly used for quality consistency evaluation of herbal medicines. Recently, an improved chromatographic technique resulted in ultra high performance liquid chromatography (UHPLC), which could provide higher resolution in less time under higher pressure using finer particles (less than 2μm) of stationary phase. A simple and sensitive method was developed and validated for fingerprint analysis of Penthorum chinense Pursh (PC), with the simultaneous determination of seven components using UPLC coupled with a diode-array detector (DAD). It took less than 20 min for analysis of one sample. Both similarity analysis and principle components analysis (PCA) were employed to evaluate the quality consistency of 17 sample batches. The analysis was performed on a Waters ACQUITY UPLC HSS T3 (2.1 × 150 mm, 1.7 μm) column, which was maintained at 45°C and the eluents were monitored with DAD at 270 nm. A gradient elution with acetonitrile and water containing 0.075% phosphoric acid was used. The solvent flow rate was 0.4 mL/min. Standard calibration curves showed good linear behavior (R2>0.9994) in the range of 0.20-337.05 μg/mL. Acceptable repeatability (RSD<0.61%), reproducibility (RSD<2.72%), stability (RSD<1.59%) and recovery in the range of 94.7%-102.9% were obtained (precision and accuracy). The validated method was successfully applied to evaluate the quality of 21 samples of PC.

scholarly journals Quantitative Determination of Triterpenes from Amphiptherygium adstringens by Liquid Chromatography and Thin-Layer Chromatography and Morphological Analysis of Cuachalalate Preparations

2006 ◽  
Vol 89 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Andrés Navarrete ◽  
Bharathi Avula ◽  
Vaishali C Joshi ◽  
Xiuhong Ji ◽  
Paul Hersh ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Gastric Ulcers ◽  
Array Detector ◽  
Plant Materials ◽  
Relative Standard ◽  
Layer Chromatography

Abstract Amphiptherygium adstringens (Anacardiaceae/Julianaceae), local name cuachalalate, is used in folk medicine for the treatment of cholelithiasis, fevers, fresh wounds, hypercholesterolemia, gastritis, gastric ulcers, and cancer of the gastrointestinal tract. The development of column high-performance liquid chromatographyphotodiode array detector (LC-PDA) and high-performance thin-layer chromatography (HPTLC)densitometry methods for the determination of masticadienonic acid and 3-hydroxymasticadienonic acid in cuachalalate preparations is described in this paper. Good separation of the compounds could be achieved by both methods. Either might be preparable depending on the requirements. The LC separation was performed on a Phenomenex Synergi MAX-RP 80A reversed-phase column operated at 40C with detection at 215 nm. The plant materials were extracted with methanol by sonication. The triterpenes present in the plant material and commercial extracts were separated with an acetonitrilewater reagent alcohol isocratic system. The limit of detection was 0.10.2 g/mL. The relative standard deviation values for the determination of triterpenes in plant extracts were less than 1.00%. This is the first report of an analytical method developed for the quantitative analysis of triterpenes from Amphiptherygium adstringens by LC-PDA and HPTLC. The stem bark showed higher amounts of triterpenes, and low amounts in root and stem root. The microscopic description of the crude drug of cuachalalate was also provided.

scholarly journals DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPTLC METHOD FOR ESTIMATION OF SWERTIAMARIN IN BULK AND DOSAGE FORM

2017 ◽  
Vol 9 (6) ◽  
pp. 80
Author(s):  
H. Padh ◽  
S. Parmar ◽  
B. Patel
Keyword(s):  
High Performance ◽  
Forced Degradation ◽  
Stability Indicating ◽  
Bulk Drug ◽  
Development And Validation ◽  
Layer Chromatography ◽  
Herbal Formulations ◽  
Hptlc Method ◽  
Ich Guideline

Objective: In the present study a novel stability-indicating high-performance thin-layer chromatography (HPTLC) method for quantitative determination of Swertiamarin (SW) in bulk drug and formulation has been developed and validated as per ICH guideline Q2 (R1) for global acceptance of standardized herbal formulations.Methods: HPTLC method is developed and validated using solvent ethyl acetate: ethanol: chloroform (3:2.5:4.5 v/v/v) (Rf of SW 0.65±0.04) in the absorbance mode at 243 nm. Various forced degradation conditions were used to check degradation of drug.Results: The method showed a good linear relationship (r2 = 0.9990) in the concentration range 200-700 ng per spot. It was found to be linear, accurate, precise and specific.Conclusion: It can be applied for quality control as well as for stability testing of different dosage forms containing swertiamarin. The developed method is validated as per ICH guideline Q2(R1) for global acceptance of standardized herbal formulations.

Chromatographic Fingerprint Analysis of Macrothelypteris torresiana and Simultaneous Determination of Several Main Constituents by LC

2009 ◽  
Vol 70 (1-2) ◽  
pp. 117-124 ◽  
Author(s):  
Chaomei Xiong ◽  
Jinlan Ruan ◽  
Ying Tang ◽  
Yaling Cai ◽  
Wei Fang ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprint
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