Effects of aerobic exercise on lipopolysaccharide induced experimental acute lung injury in the animal model of type 1 diabetes mellitus

This study evaluated the aerobic exercise effects of moderate and progressive intensity on renal function and structure, and oxidative stress in ovariectomized rats with type 1 diabetes mellitus induced by streptozotocin (STZ). Eighteen Wistar rats were divided into 3 groups: OSC - ovariectomized and sedentary control rats; OSD - ovariectomized and sedentary diabetic rats; and OTD - ovariectomized and trained diabetic rats. After induction of diabetes, the OTD group was submitted to eight weeks of exercise. Twenty-four hours after the last training session urine samples were collected. Blood samples and kidneys were collected after euthanasia for renal function analysis, histology, morphometry and oxidative stress. Our results have shown a reduction of the weight gain, increase of kidney weight and postprandial glycemia in diabetic rats. However, exercise decreased glycosuria and prevented the proteinuria in OTD group rats. Focal segmental glomerulosclerosis (FSGS), juxtamedullary glomerular tuft area, tubulointerstitial lesions (TIL), brush border loss and tubular cell debridement were reduced in OTD rats. In addition, exercise training decreased urinary and plasma concentrations of thiobarbituric acid reactive substance (TBARS). Our results demonstrate the beneficial effect of progressive aerobic exercise on proteinuria, glycosuria, and renal structure in ovariectomized diabetic rats, which may be mediated in part by reduction of oxidative stress.

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Morphological assessment of pancreatic islet hormone content following aerobic exercise training in rats with poorly controlled Type 1 diabetes mellitus

Islets ◽

10.4161/isl.29221 ◽

2014 ◽

Vol 6 (2) ◽

pp. e29221 ◽

Cited By ~ 5

Author(s):

Matthew W McDonald ◽

Michael R Murray ◽

Katharine E Hall ◽

Earl G Noble ◽

CW James Melling

Keyword(s):

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Exercise Training ◽

Aerobic Exercise ◽

Type 1 Diabetes Mellitus ◽

Pancreatic Islet ◽

Aerobic Exercise Training ◽

Islet Hormone ◽

Morphological Assessment

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In vivo Labeling of Bone Microdamage in an Animal Model of Type 1 Diabetes Mellitus

Scientific Reports ◽

10.1038/s41598-019-53487-6 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Sahar Mohsin ◽

Suneesh Kaimala ◽

Eman Khamis Yousef AlTamimi ◽

Saeed Tariq ◽

Ernest Adeghate

Keyword(s):

Diabetes Mellitus ◽

Animal Model ◽

Type 1 Diabetes ◽

Type 1 Diabetes Mellitus ◽

Bone Strength ◽

Bone Quality ◽

Increase Fracture Risk ◽

Bone Microdamage

AbstractType 1 diabetes mellitus (DM1) is linked to a decrease in bone strength. Bone strength entails both bone mineral density and bone quality. Limited data are available regarding diabetes-induced microdamage, which can severely influence bone quality. This study has investigated bone microdamage as a measure of bone quality in an animal model of DM1. Microdamage in the neck of the femur was labelled in vivo using multiple fluorochromes at 4, 12 and 24 weeks after the onset of DM1. Microcracks were quantified and their morphology analyzed using microscopy techniques. The mean length of microcracks at 24 weeks, and crack numerical and surface densities were significantly higher (p < 0.05) 4 weeks after the onset of DM1 when compared with control. Diffuse damage density was highest at 12 weeks after the onset of DM1. The arrangement of the collagen fibrils became progressively more irregular from 4 to 24 weeks of DM. This is the first study to analyze microdamage in vivo at different time points of DM1. DM1is associated with microcracks from the early stage, however bone microstructure shows toughening mechanisms that arrest their growth but disease progression further deteriorates bone quality resulting in longer microcracks which may increase fracture risk.

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