scholarly journals Bleaching of mouse rods: microspectrophotometry and suction-electrode recording

2012 ◽  
Vol 590 (10) ◽  
pp. 2353-2364 ◽  
Author(s):  
S. Nymark ◽  
R. Frederiksen ◽  
M. L. Woodruff ◽  
M. C. Cornwall ◽  
G. L. Fain

1976 ◽  
pp. 73-79 ◽  
Author(s):  
J. Cabasson ◽  
P. Puech


2018 ◽  
Vol 671 ◽  
pp. 103-107 ◽  
Author(s):  
Vladimir Rancic ◽  
Bijal Rawal ◽  
Bogdan Panaitescu ◽  
Araya Ruangkittisakul ◽  
Klaus Ballanyi




1995 ◽  
Vol 106 (3) ◽  
pp. 543-557 ◽  
Author(s):  
M C Cornwall ◽  
H R Matthews ◽  
R K Crouch ◽  
G L Fain

We have used suction electrode recording together with rapid steps into 0.5 mM IBMX solution to investigate changes in guanylyl cyclase velocity produced by pigment bleaching in isolated cones of the salamander Ambystoma tigrinum. Both backgrounds and bleaches accelerate the time course of current increase during steps into IBMX. We interpret this as evidence that the velocity of the guanylyl cyclase is increased in background light or after bleaching. Our results indicate that cyclase velocity increases nearly linearly with increasing percent pigment bleached but nonlinearly (and may saturate) with increasing back-ground intensity. In cones (as previously demonstrated for rods), light-activated pigment and bleached pigment appear to have somewhat different effects on the transduction cascade. The effect of bleaching on cyclase rate is maintained for at least 15-20 min after the light is removed, much longer than is required after a bleach for circulating current and sensitivity to stabilize in an isolated cone. The effect on the cyclase rate can be completely reversed by treatment with liposomes containing 11-cis retinal. The effects of bleaching can also be partially reversed by beta-ionone, an analogue of the chromophore 11-cis-retinal which does not form a covalent attachment to opsin. Perfusion of a bleached cone with beta-ionone produces a rapid increase in circulating current and sensitivity, which rapidly reverses when the beta-ionone is removed. Perfusion with beta-ionone also causes a partial reversal of the bleach-induced acceleration of cyclase velocity. We conclude that bleaching produces an "equivalent background" excitation of the transduction cascade in cones, perhaps by a mechanism similar to that in rods.



2008 ◽  
Vol 1218 ◽  
pp. 54-69 ◽  
Author(s):  
Markus Kessler ◽  
Bryant Kiliman ◽  
Carrie Humes ◽  
Amy C. Arai


2000 ◽  
Vol 88 (4) ◽  
pp. 1489-1495 ◽  
Author(s):  
David F. Donnelly ◽  
Ricardo Rigual

A preparation was developed that allows for the recording of single-unit chemoreceptor activity from mouse carotid body in vitro. An anesthetized mouse was decapitated, and each carotid body was harvested, along with the sinus nerve, glossopharyngeal nerve, and petrosal ganglia. After exposure to collagenase/trypsin, the cleaned complex was transferred to a recording chamber where it was superfused with oxygenated saline. The ganglia was searched for evoked or spontaneous unit activity by using a glass suction electrode. Single-unit action potentials were 57 ± 10 (SE) ( n = 16) standard deviations above the recording noise, and spontaneous spikes were generated as a random process. Decreasing superfusate[Formula: see text] to near 20 Torr caused an increase in spiking activity from 1.3 ± 0.4 to 14.1 ± 1.9 Hz ( n = 16). The use of mice for chemoreceptor studies may be advantageous because targeted gene deletions are well developed in the mouse model and may be useful in addressing unresolved questions regarding the mechanism of chemotransduction.





2022 ◽  
Vol 3 (1) ◽  
pp. 101032
Author(s):  
Yan Tang ◽  
Diego Benusiglio ◽  
Arthur Lefevre ◽  
Stephanie Küppers ◽  
Olga Lapies ◽  
...  


2021 ◽  
Vol 12 ◽  
pp. 379
Author(s):  
Nobutaka Mukae ◽  
Daisuke Kuga ◽  
Daisuke Murakami ◽  
Noritaka Komune ◽  
Yusuke Miyamoto ◽  
...  

Background: Temporal lobe epilepsy (TLE) associated with temporal lobe encephalocele is rare, and the precise epileptogenic mechanisms and surgical strategies for such cases are still unknown. Although the previous studies have reported good seizure outcomes following chronic subdural electrode recording through invasive craniotomy, only few studies have reported successful epilepsy surgery through endoscopic endonasal lesionectomy. Case Description: An 18-year-old man developed generalized convulsions at the age of 15 years. Despite treatment with optimal doses of antiepileptic drugs, episodes of speech and reading difficulties were observed 2–3 times per week. Long-term video electroencephalogram (EEG) revealed ictal activities starting from the left anterior temporal region. Magnetic resonance imaging revealed a temporal lobe encephalocele in the left lateral fossa of the sphenoidal sinus (sphenoidal encephalocele). Through the endoscopic endonasal approach, the tip of the encephalocele was exposed. A depth electrode was inserted into the encephalocele, which showed frequent spikes superimposed with high-frequency oscillations (HFOs) suggesting intrinsic epileptogenicity. The encephalocele was resected 8 mm from the tip. Twelve months postoperatively, the patient had no recurrence of seizures on tapering of the medication. Conclusion: TLE associated with sphenoidal encephalocele could be controlled with endoscopic endonasal lesionectomy, after confirming the high epileptogenicity with analysis of HFOs of intraoperative EEG recorded using an intralesional depth electrode.



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