A novel suction electrode recording technique for monitoring circadian rhythms in single and multiunit discharge from brain slices

2006 ◽  
Vol 156 (1-2) ◽  
pp. 173-181 ◽  
Author(s):  
Timothy M. Brown ◽  
Jon R. Banks ◽  
Hugh D. Piggins

2018 ◽  
Vol 671 ◽  
pp. 103-107 ◽  
Author(s):  
Vladimir Rancic ◽  
Bijal Rawal ◽  
Bogdan Panaitescu ◽  
Araya Ruangkittisakul ◽  
Klaus Ballanyi


1976 ◽  
pp. 73-79 ◽  
Author(s):  
J. Cabasson ◽  
P. Puech


2012 ◽  
Vol 590 (10) ◽  
pp. 2353-2364 ◽  
Author(s):  
S. Nymark ◽  
R. Frederiksen ◽  
M. L. Woodruff ◽  
M. C. Cornwall ◽  
G. L. Fain


1995 ◽  
Vol 106 (3) ◽  
pp. 543-557 ◽  
Author(s):  
M C Cornwall ◽  
H R Matthews ◽  
R K Crouch ◽  
G L Fain

We have used suction electrode recording together with rapid steps into 0.5 mM IBMX solution to investigate changes in guanylyl cyclase velocity produced by pigment bleaching in isolated cones of the salamander Ambystoma tigrinum. Both backgrounds and bleaches accelerate the time course of current increase during steps into IBMX. We interpret this as evidence that the velocity of the guanylyl cyclase is increased in background light or after bleaching. Our results indicate that cyclase velocity increases nearly linearly with increasing percent pigment bleached but nonlinearly (and may saturate) with increasing back-ground intensity. In cones (as previously demonstrated for rods), light-activated pigment and bleached pigment appear to have somewhat different effects on the transduction cascade. The effect of bleaching on cyclase rate is maintained for at least 15-20 min after the light is removed, much longer than is required after a bleach for circulating current and sensitivity to stabilize in an isolated cone. The effect on the cyclase rate can be completely reversed by treatment with liposomes containing 11-cis retinal. The effects of bleaching can also be partially reversed by beta-ionone, an analogue of the chromophore 11-cis-retinal which does not form a covalent attachment to opsin. Perfusion of a bleached cone with beta-ionone produces a rapid increase in circulating current and sensitivity, which rapidly reverses when the beta-ionone is removed. Perfusion with beta-ionone also causes a partial reversal of the bleach-induced acceleration of cyclase velocity. We conclude that bleaching produces an "equivalent background" excitation of the transduction cascade in cones, perhaps by a mechanism similar to that in rods.



2006 ◽  
Vol 19 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Myriam Juda ◽  
Mirjam Münch ◽  
Anna Wirz-Justice ◽  
Martha Merrow ◽  
Till Roenneberg

Abstract: Among many other changes, older age is characterized by advanced sleep-wake cycles, changes in the amplitude of various circadian rhythms, as well as reduced entrainment to zeitgebers. These features reveal themselves through early morning awakenings, sleep difficulties at night, and a re-emergence of daytime napping. This review summarizes the observations concerning the biological clock and sleep in the elderly and discusses the documented and theoretical considerations behind these age-related behavioral changes, especially with respect to circadian biology.



1982 ◽  
Vol 27 (12) ◽  
pp. 931-932
Author(s):  
Gerald S. Wasserman
Keyword(s):  




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