Comparison of Temperature Metrics of Heating-Induced Damage

2002 ◽  
Author(s):  
Neil T. Wright
Keyword(s):  
Thermal Denaturation ◽  
Transition State Theory ◽  
Type I Collagen ◽  
Arrhenius Equation ◽  
Frequency Factor ◽  
State Theory ◽  
Type I ◽  
Denaturation Temperature ◽  
First Order ◽  
First Order Phase

The temperature dependence of the rate of denaturation of Type I collagen due to heating is described well by chemical kinetics via the Arrhenius equation or transition state theory (TST) [1, 2, 3], each of which requires two material parameters. Nevertheless, many have sought to find a single convenient metric, such as one with units of temperature, to describe thermal denaturation of collagen. Comparing the results of studies that measured denaturation and cell death for a variety of biological samples shows that the parameters for either the Arrhenius equation (i.e. activation energy Ea and the frequency factor A) appear correlated over the range of temperatures for which biological materials are tested [4]. It has also been suggested that denaturation is a first-order phase change (i.e., melting) and thus should be characterized by a melting or denaturation temperature Td [5].

Thermal denaturation of type I collagen vitrified gels

2012 ◽  
Vol 527 ◽  
pp. 172-179 ◽  
Author(s):  
Zhiyong Xia ◽  
Xiomara Calderon-Colon ◽  
Morgana Trexler ◽  
Jennifer Elisseeff ◽  
Qiongyu Guo
Keyword(s):  
Thermal Denaturation ◽  
Type I Collagen ◽  
Type I

scholarly journals Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 213
Author(s):  
Chie Kojima ◽  
Misaki Nishio ◽  
Yusuke Nakajima ◽  
Takeshi Kawano ◽  
Kenji Takatsuka ◽  
...  
Keyword(s):  
Single Cell ◽  
Gold Nanoparticle ◽  
Thermal Denaturation ◽  
Collagen Gel ◽  
Collagen Fibers ◽  
Photothermal Effect ◽  
Cell Detachment ◽  
Type I ◽  
Collagen Gels ◽  
Denaturation Temperature

Cell Separation is important in various biomedical fields. We have prepared gold nanoparticle (AuNP)-embedded collagen gels as a visible-light-responsive cell scaffold in which photoinduced single cell detachment occurs through local thermal denaturation of the collagen gel via the photothermal effect of AuNP. Physicochemical properties of collagen materials depend on the origin of the collagen and the presence of telopeptides. In this study, we prepared various AuNP-embedded collagen gels by using different collagen materials with and without the telopeptides to compare their thermal denaturation properties and photoinduced single cell detachment behaviors. Cellmatrix type I-C without telopeptides exhibited a lower denaturation temperature than Cellmatrix type I-A and Atelocell IAC, as examined by Fourier transform infrared (FTIR) spectroscopy, rheological analysis, and sol–gel transition observation. Three-dimensional (3D) laser microscopic imaging revealed that collagen fibers shrank in Cellmatrix type I-A upon heating, but collagen fibers disappeared in Cellmatrix type I-C upon heating. Cells cultured on the Cellmatrix type I-C-based AuNP-embedded collagen gel detached with shorter photoirradiation than on the Cellmatrix type I-A-based AuNP-embedded collagen gel, suggesting that collagen gels without telopeptides are suitable for a photoinduced single cell detachment system.

scholarly journals PHASE TRANSITIONS FOR A ϕ6 MODEL IN (2+1)-DIMENSIONAL CURVED SPACETIME

2003 ◽  
Vol 18 (13) ◽  
pp. 937-946 ◽  
Author(s):  
MINU JOY ◽  
V. C. KURIAKOSE
Keyword(s):  
Phase Transitions ◽  
Effective Potential ◽  
Momentum Tensor ◽  
Type I ◽  
Curved Spacetime ◽  
First Order ◽  
Spacetime Curvature ◽  
First Order Phase Transition ◽  
The One ◽  
First Order Phase

Considering a massive ϕ6 self-interacting scalar field coupled arbitrarily to a (2+1)-dimensional Bianchi type-I spacetime, we evaluate the one-loop effective potential. It is found that ϕ6 potential can be regularized in (2+1)-dimensional curved spacetime. A finite expression for the energy–momentum tensor is obtained for this model. Evaluating the finite temperature effective potential, the temperature dependence of phase transitions is studied. The crucial dependence of the phase transitions on the spacetime curvature and on the coupling to gravity is also studied. The nature of phase transitions for the present model is clarified to be first order. A first-order phase transition proceeds by nucleation of bubbles of broken phase in the background of unbroken phase.

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