scholarly journals Evaluation of HIV Western Blot Test Kits for HIV Confirmatory Diagnosis in Japan

2019 ◽  
Vol 93 (1) ◽  
pp. 12-17
Author(s):  
Shigeru KUSAGAWA
Keyword(s):  
Western Blot ◽  
Western Blot Test

A Preliminary Evaluation of a Prototype Western Blot Confirmatory Test Kit for Syphilis

1994 ◽  
Vol 5 (6) ◽  
pp. 409-414 ◽  
Author(s):  
H Young ◽  
P J Walker ◽  
D Merry ◽  
A Mifsud
Keyword(s):  
Western Blot ◽  
False Positive ◽  
High Sensitivity ◽  
High Specificity ◽  
Confirmatory Test ◽  
Preliminary Evaluation ◽  
Serological Tests ◽  
Western Blot Test ◽  
Specificity And Sensitivity ◽  
Test Kit

A prototype Western blot kit was evaluated as a confirmatory test for syphilis using 131 sera characterized by other serological tests for syphilis. There were 114 treponemal sera (including 94 cases of early syphilis, 83 of which were untreated) and 17 non-treponemal problem sera (11 gave false positive reactions on screening with the TmpA recombinant antigen enzyme immunoassay (EIA), 3 gave false positive fluorescent treponemal antibody absorbed (FTA-abs) tests, and 3 false positive Captia Syphilis G EIA results). Based on the manufacturer's criteria of reactivity in multiple bands for designating a positive result the Western blot test gave a sensitivity of 99.1% (113/114) and a specificity of 88.2% (15/17) when indeterminate reactions were scored positive and 98.2% (112/114) and 100% (17/17) when indeterminate reactions were scored negative. Sensitivity was high in both treated and untreated infection. Corresponding sensitivities for the TPHA and FTA-abs when equivocal reactions were scored negative were 97.5% (111/114) and 99.1% (113/114). The high sensitivity of the FTA-abs in this study is probably due to the large number of untreated primary infections. Our results with the Western blot, confirm earlier studies using ‘in-house’ test systems and, support a role for a commercial Western blot test in the confirmatory diagnosis of syphilis. Further studies are required to confirm the high specificity and sensitivity of the kit in a larger series including a wider variety of non-treponemal cases as well as patients with untreated and treated infection.

scholarly journals Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate

2008 ◽  
Vol 41 (4) ◽  
pp. 325-329 ◽  
Author(s):  
Ana Paula Ferreira ◽  
Thadeu Côrrea ◽  
Rosângela Cunha ◽  
Marcos José Marques ◽  
Maria Angela Montesano ◽  
...  
Keyword(s):  
Western Blot ◽  
Human Serum ◽  
Paracoccidioides Brasiliensis ◽  
Serum Antibody ◽  
Parasitic Infections ◽  
Sodium Metaperiodate ◽  
Chronic Form ◽  
Western Blot Test ◽  
Immunoglobulin Isotypes ◽  
Acute Form

In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis.

Evaluation of a Western Blot Test, Helico Blot 2.1, in the Diagnosis of Helicobacter pylori Infection in a Pediatric Population

Helicobacter ◽  
2002 ◽  
Vol 7 (3) ◽  
pp. 210-215 ◽  
Author(s):  
Monica Oleastro ◽  
Rita Matos ◽  
Jose Cabral ◽  
Rosa Barros ◽  
Ana Isabel Lopes ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Western Blot ◽  
Pediatric Population ◽  
Helicobacter Pylori Infection ◽  
Western Blot Test

Instruction of feeding methods to Japanese pregnant women who cannot be confirmed as HTLV-1 carrier by western blot test

2013 ◽  
Vol 27 (13) ◽  
pp. 1392-1393 ◽  
Author(s):  
Shunji Suzuki ◽  
Masanobu Tanaka ◽  
Hideo Matsuda ◽  
Yuki Tsukahara ◽  
Yasushi Kuribayashi ◽  
...  
Keyword(s):  
Western Blot ◽  
Pregnant Women ◽  
Western Blot Test ◽  
Feeding Methods ◽  
Japanese Pregnant Women

HIV western blot test

1994 ◽  
Vol 160 (12) ◽  
pp. 808-808
Author(s):  
Elizabeth Dax
Keyword(s):  
Western Blot ◽  
Western Blot Test

scholarly journals Standardization and validation of a western blot for the diagnosis of human immunodeficiency virus

2021 ◽  
Vol 21 (4) ◽  
pp. 674-681
Author(s):  
Eduardo F. Miranda Ulloa ◽  
Soledad Romero Ruiz ◽  
Bernardina Amorín Uscata ◽  
Kevin Serrano Segura ◽  
Ronal Briceño Espinoza ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Western Blot ◽  
Diagnostic Performance ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Reference Test ◽  
Western Blot Test ◽  
Immunodeficiency Virus ◽  
Sensitivity Specificity ◽  
Hiv 1

Objectives: To standardize and validate a western blot test for the diagnosis of human immunodeficiency virus. Methods: A prospective observational study was carried out during 2017 and 2018. The western blot test was standardized, using the polyacrylamide gel electrophoresis technique with sodium dodecyl sulfate (SDS PAGE), being the nitrocellulose blot strips prepared with an Optimal HIV-1 antigen concentration of 2.71 µg / mm. The western blot was validated in the laboratory against 400 reference samples (300 sera and 100 plasmas): 200 positive and 200 negatives for antibodies against HIV-1, being the reference test the Immunoblot of the Fujirebio brand. Diagnostic performance parameters were estimated using Epidat v3.1 and Excel. Results: Eight important bands of the HIV-1 antigen were identified: p17, p24, p31, p39, gp41, p55, p66, and gp120. According to the Consortium for the normalization of serology for retroviruse, those that were taken as specific diagnostic bands were: p24, p31, gp41, and gp120. The sensitivity, specificity, positive and negative predictive value and validity index against sera were: 96.7%, 96.0%, 96.0%, 96.6%, 96.3%; and against plasmas: 98.0%, 100.0%, 100.0%, 98.0%, 99.0% respectively. No false positives and negatives were found, but some were undetermined. Conclusion: The development of this western blot test with proprietary technology presented similar diagnostic performance to the reference test, without showing cross-reactions, being useful for confirming HIV.

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