scholarly journals Distribution of Clinical Isolates of Serratia marcescens by Bacteriocin Typing

1984 ◽  
Vol 58 (4) ◽  
pp. 312-317
Author(s):  
Masaru NASU ◽  
Jun GOTO ◽  
Yoichiro GOTO ◽  
Takayoshi TASHIRO ◽  
Takashi ITOGA ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Clinical Isolates ◽  
Bacteriocin Typing

scholarly journals The Serratia marcescens Siderophore Serratiochelin Is Necessary for Full Virulence during Bloodstream Infection

2020 ◽  
Vol 88 (8) ◽  
Author(s):  
Danelle R. Weakland ◽  
Sara N. Smith ◽  
Bailey Bell ◽  
Ashootosh Tripathi ◽  
Harry L. T. Mobley
Keyword(s):  
Serratia Marcescens ◽  
Bloodstream Infection ◽  
Iron Acquisition ◽  
Gene Clusters ◽  
Clinical Isolates ◽  
Wild Type ◽  
Serratia Plymuthica ◽  
Content Type ◽  
Cas Assay ◽  
Essential Nutrient

ABSTRACT Serratia marcescens is a bacterium frequently found in the environment, but over the last several decades it has evolved into a concerning clinical pathogen, causing fatal bacteremia. To establish such infections, pathogens require specific nutrients; one very limited but essential nutrient is iron. We sought to characterize the iron acquisition systems in S. marcescens isolate UMH9, which was recovered from a clinical bloodstream infection. Using RNA sequencing (RNA-seq), we identified two predicted siderophore gene clusters (cbs and sch) that were regulated by iron. Mutants were constructed to delete each iron acquisition locus individually and in conjunction, generating both single and double mutants for the putative siderophore systems. Mutants lacking the sch gene cluster lost their iron-chelating ability as quantified by the chrome azurol S (CAS) assay, whereas the cbs mutant retained wild-type activity. Mass spectrometry-based analysis identified the chelating siderophore to be serratiochelin, a siderophore previously identified in Serratia plymuthica. Serratiochelin-producing mutants also displayed a decreased growth rate under iron-limited conditions created by dipyridyl added to LB medium. Additionally, mutants lacking serratiochelin were significantly outcompeted during cochallenge with wild-type UMH9 in the kidneys and spleen after inoculation via the tail vein in a bacteremia mouse model. This result was further confirmed by an independent challenge, suggesting that serratiochelin is required for full S. marcescens pathogenesis in the bloodstream. Nine other clinical isolates have at least 90% protein identity to the UMH9 serratiochelin system; therefore, our results are broadly applicable to emerging clinical isolates of S. marcescens causing bacteremia.

Comparison of 3 Alcohol Gels and 70% Ethyl Alcohol for Hand Hygiene

2008 ◽  
Vol 29 (10) ◽  
pp. 960-962 ◽  
Author(s):  
Mirian Nicéa Zarpellon ◽  
Vanessa Sarto Soares ◽  
Natal Rodrigo Albrecht ◽  
Douglas Ricardo da Silva Bergamasco ◽  
Lourdes Botelho Garcia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Hand Hygiene ◽  
Serratia Marcescens ◽  
Ethyl Alcohol ◽  
Laboratory Study ◽  
Clinical Isolates ◽  
Methicillin Resistant ◽  
Human Volunteers

In a laboratory study, we demonstrated that 3 alcohol-based hand gels, commercially available in Brazil, were as effective as the traditional 70% ethyl alcohol (by weight) in removing clinical isolates of methicillin-resistant Staphylococcus aureus, Serratia marcescens, and Candida albicans from heavily contaminated hands of human volunteers.

scholarly journals Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens

1997 ◽  
Vol 30 (11) ◽  
pp. 1291-1298 ◽  
Author(s):  
G.V. Carbonell ◽  
A.F. Alfieri ◽  
A.A. Alfieri ◽  
M.C. Vidotto ◽  
C.E. Levy ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Serratia Marcescens ◽  
Vero Cells ◽  
Clinical Isolates

Identification of plasmid- and integron-borne bla IMP-1 and bla IMP-10 in clinical isolates of Serratia marcescens

2009 ◽  
Vol 58 (2) ◽  
pp. 217-221 ◽  
Author(s):  
Zhuting Hu ◽  
Wei-Hua Zhao
Keyword(s):  
Serratia Marcescens ◽  
Single Point ◽  
Gene Cassette ◽  
Clinical Isolates ◽  
Single Point Mutation ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
E Coli ◽  
Class 1 ◽  
P2 Promoter

The emergence of carbapenem-hydrolysing metallo-β-lactamases (MBLs) is a serious threat to the clinical utility of carbapenems. This study identified plasmid- and integron-borne bla IMP-1 and bla IMP-10 in clinical isolates of Serratia marcescens. The bla IMP-1 and bla IMP-10 gene cassettes were carried by a class 1 integron and followed by the aac(6′)-IIc gene cassette. The bla IMP-1 and bla IMP-10 gene cassettes were preceded by a weak Pant promoter, TGGACA(N)17TAAGCT, and an inactive P2 promoter, TTGTTA(N)14TACAGT. These genes were easily transferred to Escherichia coli by conjugation and transformation, indicating that they are located on transferable plasmids. Due to the acquisition of bla IMP-1, the susceptibility of E. coli transconjugants to imipenem, meropenem, panipenem and biapenem decreased by 32-, 256-, 64- and 128-fold, respectively. In comparison, after gaining bla IMP-10, the susceptibility of E. coli transconjugants to the four carbapenems decreased by 64-, 2048-, 256- and 64-fold, respectively. Strains harbouring bla IMP-10 showed higher-level resistance to imipenem, meropenem and panipenem than the strains harbouring bla IMP-1, although the nucleotide sequences of the class 1 integrons carrying bla IMP-10 and bla IMP-1 were identical except for a single point mutation.

Sign in / Sign up

Export Citation Format

Share Document