Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Urine Samples of Males and Females by the Strand Displacement Amplification (SDA) Method

Introduction: The rate of transmission of Chlamydia trachomatis, by infected males and females to their sexual partners, has been a matter of continued scientific interest and exploration. Methods: We examined the correlation of C. trachomatis infection in sexual partnerships, using Strand Displacement Amplification. Results: During July–November 2003, 97 male patients were reported positive for C. trachomatis. Fifty of the female sexual consorts were amenable for contact tracing, 38 of whom were identified as C. trachomatis positive. Within the same period, 93 female patients were C. trachomatis positive, and 56 male consorts were traceable, of whom 43 were positive for C. trachomatis. Conclusions: The concordance of C. trachomatis between sexual partners is in the region of 75%. This strengthens the case for epidemiological treatment for all consorts.

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Take three, test one: a cross-sectional study to evaluate the molecular detection of Chlamydia trachomatis and Neisseria gonorrhoeae in pooled pharyngeal, anorectal and urine samples versus single-site testing among men who have sex with men in Belgium

Acta Clinica Belgica ◽

10.1080/17843286.2018.1545376 ◽

2018 ◽

Vol 75 (2) ◽

pp. 91-95 ◽

Cited By ~ 6

Author(s):

Irith De Baetselier ◽

Kara Krista Osbak ◽

Hilde Smet ◽

Chris Richard Kenyon ◽

Tania Crucitti

Keyword(s):

Chlamydia Trachomatis ◽

Neisseria Gonorrhoeae ◽

Molecular Detection ◽

Cross Sectional Study ◽

Single Site ◽

Urine Samples ◽

Sectional Study ◽

Site Testing ◽

Cross Sectional ◽

Sex With Men

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Pooling of urine samples for molecular detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium as a screening strategy among young adults in Catalonia

Enfermedades infecciosas y microbiologia clinica (English ed ) ◽

10.1016/j.eimce.2019.05.006 ◽

2020 ◽

Vol 38 (2) ◽

pp. 65-71

Author(s):

Evelin López-Corbeto ◽

Victoria González ◽

Rossie Lugo ◽

Belen Rivaya ◽

Jordi Casabona ◽

...

Keyword(s):

Young Adults ◽

Chlamydia Trachomatis ◽

Neisseria Gonorrhoeae ◽

Molecular Detection ◽

Mycoplasma Genitalium ◽

Screening Strategy ◽

Urine Samples

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Risk of Coinfection withChlamydia trachomatisandNeisseria Gonorrhoeaein Nova Scotia

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2010/760218 ◽

2010 ◽

Vol 21 (2) ◽

pp. e84-e86 ◽

Cited By ~ 13

Author(s):

Kevin R Forward

Keyword(s):

Nova Scotia ◽

Chlamydia Trachomatis ◽

Neisseria Gonorrhoeae ◽

Treatment Decisions ◽

Empirical Treatment ◽

Prevalence Rates ◽

Accession Number ◽

Males And Females ◽

Concurrent Testing

BACKGROUND: The frequency of Chlamydia trachomatis and Neisseria gonorrhoeae coinfection can vary depending on their individual incidence and prevalence rates.OBJECTIVE: To determine the frequency ofC trachomatisandN gonorrhoeaecoinfections by evaluating the results of testing in 2007 and 2008 to better inform testing and treatment decisions.METHODS: Specimens from the same patient submitted on the same day served as the basis for the present study. The age, sex and the source of the specimen were also linked to the accession number. Infection and coinfection rates were analyzed in both males and females.RESULTS: Concurrent testing was performed on 41,567 female specimens and 1827 male specimens, of which, 1495 female samples (3.6%) tested positive forC trachomatisinfection and 88 (0.2%) tested positive forN gonorrhoeaeinfections. Only 31 females were coinfected; however, for those between 11 and 25 years of age, 25 of 61 females (40.1%) withN gonorrhoeaeinfection also tested positive forC trachomatisinfection; conversely, 25 of 1248 females (2.0%) withC trachomatisinfection also tested positive forN gonorrhoeaeinfection. For males, 213 (11.7%) tested positive forC trachomatisinfection,and 59 (3.2%) tested positive forN gonorrhoeaeinfection. In 30 males withN gonorrhoeaebetween 11 and 25 years of age, and 149 males withC trachomatis,eight coinfections were observed (26.7% and 5.3%, respectively). Of those older than 25 years of age, only five of 905 men and six of 19,465 women were coinfected. None of the 10,935 women who were 30 years of age or older had coinfections.CONCLUSION: TheN gonorrhoeaecoinfection rate in males withC trachomatismay justify empirical antimicrobials; however, in females, the proportion of coinfected may not justify empirical treatment forN gonorrhoeaeinfection when theC trachomatistest is positive andN gonorrhoeaetesting has not been performed.

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