WITHDRAWN: “Strand displacement amplification (SDA) for chlamydia trachomatis detection in rectal, pharyngeal and eye swabs. Is multiple site sampling beneficial in the G.U.M setting?”

2006 ◽  
Author(s):  
A. Cope ◽  
C. Ryan ◽  
G. Kudesia ◽  
G.R. Kinghom ◽  
W. Lo
Keyword(s):  
Chlamydia Trachomatis ◽  
Multiple Site ◽  
Strand Displacement ◽  
Strand Displacement Amplification

The concordance of Chlamydia trachomatis genital infection between sexual partners, in the era of nucleic acid testing

Sexual Health ◽  
2005 ◽  
Vol 2 (1) ◽  
pp. 23 ◽  
Author(s):  
A. R. Markos
Keyword(s):  
Chlamydia Trachomatis ◽  
Sexual Partners ◽  
Contact Tracing ◽  
Genital Infection ◽  
Nucleic Acid Testing ◽  
Strand Displacement ◽  
Scientific Interest ◽  
Strand Displacement Amplification ◽  
Male Patients ◽  
Males And Females

Introduction: The rate of transmission of Chlamydia trachomatis, by infected males and females to their sexual partners, has been a matter of continued scientific interest and exploration. Methods: We examined the correlation of C. trachomatis infection in sexual partnerships, using Strand Displacement Amplification. Results: During July–November 2003, 97 male patients were reported positive for C. trachomatis. Fifty of the female sexual consorts were amenable for contact tracing, 38 of whom were identified as C. trachomatis positive. Within the same period, 93 female patients were C. trachomatis positive, and 56 male consorts were traceable, of whom 43 were positive for C. trachomatis. Conclusions: The concordance of C. trachomatis between sexual partners is in the region of 75%. This strengthens the case for epidemiological treatment for all consorts.

Performance Characteristics of the Becton Dickinson ProbeTec System for Direct Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Male and Female Urine Specimens in Comparison With the Roche Cobas System

2000 ◽  
Vol 124 (11) ◽  
pp. 1649-1652 ◽  
Author(s):  
Edward L. Chan ◽  
Ken Brandt ◽  
Karen Olienus ◽  
Nick Antonishyn ◽  
Greg B. Horsman
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Strand Displacement ◽  
Chain Reaction ◽  
Becton Dickinson ◽  
Strand Displacement Amplification ◽  
Male And Female ◽  
Female Urine ◽  
Polymerase Chain ◽  
Urine Specimens

Abstract Objective.—The Becton Dickinson BDProbeTec ET System is a new semiautomated system using strand displacement amplification technology that simultaneously amplifies and detects Chlamydia trachomatis and Neisseria gonorrhoeae DNA. The strand displacement amplification products are hybridized with a fluorescent detector probe and are captured by a chemiluminescent assay in a microwell format. An amplification control is also included to monitor assay inhibition. This study evaluated the performance of the BDProbTec ET system in detecting C trachomatis and N gonorrhoeae in male and female urine specimens, calculated its ability to process large volumes of specimens, and determined the inhibition rate. Materials and Methods.—Eight hundred twenty-five male and 399 female urine specimens were tested for both C trachomatis and N gonorrhoeae with the BDProbeTec ET system, and results were compared with those of the Roche Amplicor Cobas system. All urine specimens were processed on both assays on the same day they were received, according to the manufacturers' instructions. Discrepant results were resolved by in-house polymerase chain reaction assays. Internal or amplification controls were also used in each specimen assay to monitor inhibition. The throughput of the BDProbTec ET system was further tested with 150 urine specimens on an 8-hour shift for 2 days. Results.—The overall sensitivity, specificity, positive predicative value, and negative predicative value for for detection of chlamydia were 95.3%, 99.3%, 95.9%, and 99.2% for strand displacement amplification and 95.9%, 98.3%, 90.6%, and 99.3% for the Roche Amplicor system. For detection of gonorrhea, these values were 100%, 99.7%, 88.2%, and 100% and 96.7%, 98.9%, 69%, and 99.9%, respectively. The overall inhibition rates for both strand displacement amplification and Roche Amplicor were less than 3.5%. The BDProbTec ET system was able to produce 150 results each for chlamydia and gonorrhea and the internal control within the 8-hour shift. Conclusions.—The performance characteristics of the BDProbeTec ET assay are similar to those of the Roche Amplicor polymerase chain reaction for detection of chlamydia and gonorrhea in male and female urine specimens. The system was able to produce 300 results in an 8-hour shift.

scholarly journals RICERCA DI DNA DI CHLAMYDIA TRACHOMATIS IN CAMPIONI CLINICI MEDIANTE STRAND DISPLACEMENT AMPLIFICATION (SDA)

2005 ◽  
Vol 20 (3) ◽  
Author(s):  
A. Di Taranto ◽  
R. Del Prete ◽  
R. De Nittis ◽  
R. Antonetti ◽  
G. Miragliotta
Keyword(s):  
Chlamydia Trachomatis ◽  
Strand Displacement ◽  
Strand Displacement Amplification

scholarly journals Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 602
Author(s):  
Sandra Leonardo ◽  
Anna Toldrà ◽  
Mònica Campàs
Keyword(s):  
Food Safety ◽  
Environmental Monitoring ◽  
Rolling Circle Amplification ◽  
Dna Amplification ◽  
Isothermal Amplification ◽  
In Situ Monitoring ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Bacterial Detection ◽  
Efficient Detection

The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand, biosensors based on isothermal DNA amplification methods have emerged, which avoid the need for thermal cycling, thus facilitating their integration into small and low-cost devices for in situ monitoring. This review focuses on the breakthroughs made on biosensors based on isothermal amplification methods for the detection of bacteria in the field of food safety and environmental monitoring. Optical and electrochemical biosensors based on loop mediated isothermal amplification (LAMP), rolling circle amplification (RCA), recombinase polymerase amplification (RPA), helicase dependent amplification (HDA), strand displacement amplification (SDA), and isothermal strand displacement polymerisation (ISDPR) are described, and an overview of their current advantages and limitations is provided. Although further efforts are required to harness the potential of these emerging analytical techniques, the coalescence of the different isothermal amplification techniques with the wide variety of biosensing detection strategies provides multiple possibilities for the efficient detection of bacteria far beyond the laboratory bench.

Ultra-sensitive MicroRNA-21 detection based on multiple cascaded strand displacement amplification and CRISPR/Cpf1 (MC-SDA/CRISPR/Cpf1)

2021 ◽  
Author(s):  
Xiaolong Chen ◽  
Yuanyi Deng ◽  
Gaihua Cao ◽  
Yifan Xiong ◽  
Danqun Huo ◽  
...  
Keyword(s):  
Cancer Diagnosis ◽  
Analytical Method ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Diagnosis And Prognosis ◽  
Potential Biomarker ◽  
Cancer Diagnosis And Prognosis

MicroRNA-21 (miR-21) has been considered as a potential biomarker for cancer diagnosis and prognosis due to its highly expressed in tumors. Here, an analytical method which integrates the multiple cascaded...

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