scholarly journals Intra- and inter-muscular differences in the cross-sectional area of the quadriceps muscles assessed by extended field-of-view ultrasonography

2020 ◽  
Vol 22 (2) ◽  
pp. 152 ◽  
Author(s):  
Chrysostomos Sahinis ◽  
Eleftherios Kellis ◽  
Nikiforos Galanis ◽  
Konstantinos Dafkou ◽  
Athanasios Ellinoudis
Keyword(s):  
Vastus Lateralis ◽  
Intraclass Correlation ◽  
Cross Sectional Area ◽  
Field Of View ◽  
Cost Evaluation ◽  
Sectional Area ◽  
Cross Sectional ◽  
Vastus Intermedius ◽  
Extended Field ◽  
Extended Field Of View

Aim: Τo examine the inter- and intra-muscular differences in the anatomical cross-sectional area (CSA) of the quadricep muscles, using extended - field of view (EFOV) ultrasonography (US).Material and methods: Panoramic transverse US images of the thigh were acquired from 10 young participants at five different locations across the thigh, in two sessions, spaced a week apart. The CSA of the vastus medialis (VM), rectus femoris (RF), vastus intermedius (VI), vastus lateralis (VL) and tensor vastus intermedius (TVI) was quantified.Results: The intraclass correlation coefficients ranged from 0.75 to 0.97 and the standard error of measurement ranged from 0.78% to 6.61%, indicating high test-retest reliability. Analysis of the variance indicated that among the 5 quadriceps muscles the VL and the RF displayed the greater CSA proximally, the VI medially and the VM distally across the thigh (p <0.05). No differences in the quadriceps CSA measured with and without including the TVI were found (p >0.05).Conclusions: The EFOV US technique provides transverse scans of the quadriceps muscle in vivo and allowed a reliable and non-invasive determination of CSA at a low cost. Evaluation of CSA along the thigh largely depends on the measurement site. Future studies that examine the quadriceps CSA using EFOV after any form of intervention should consider changes of at least 6.5% as meaningful.

scholarly journals Use of Extended View Ultrasound Imaging Results in Reliable Measurement of Transversus Abdominis Muscle Size in Three Dimensions During Relaxation and a Hollowing Contraction

2020 ◽  
Author(s):  
Aaron Johnson ◽  
Lauren Adams ◽  
Jade Kho ◽  
Daniel Green ◽  
Nicolas Pace ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Cross Sectional Area ◽  
Field Of View ◽  
Transversus Abdominis ◽  
Proper Function ◽  
Sectional Area ◽  
Cross Sectional ◽  
Imaging Results ◽  
Extended Field ◽  
Extended Field Of View

Abstract Background: The strength and size of core muscles, including the abdominal muscles, are crucial to proper function in most activities. Therefore, it is important to reliably assess these characteristics. Our primary objective was to determine if the length, thickness and cross-sectional area of the transversus abdominis (TrA) can be visualized independently from the internal and external abdominal oblique muscles using extended field of view ultrasound imaging at rest and with contraction and to establish its intra- and inter-tester reliability. Methods: Twenty-six individuals were recruited to participate in the study (20 F, 6 M), average age 24.0 years (SD 9.4), height 170.7 cm (SD 8.6) and weight 63.9 kg (SD 9.0). From this total number of participants, two groups of 16 randomly selected participants were assessed to determine intra- and inter-tester reliability respectively. Extended field of view ultrasound images were obtained at three vertebral levels during rest and contraction in the side lying position for both the right and left sides of the trunk. Results: Excellent intra-tester and inter-tester reliability was seen (ICC range of 0.972 to 0.984). The overall average percent SEM for all measurements and locations was approximately 4%. The overall average minimal difference for the thickness measurement for the resting and contraction conditions combined were as follows: intratester 0.056 (0.014) cm and intertester 0.054 (0.017) cm, for area intratester 0.287 (0.086) cm2 and intertester 0.289 (0.101) cm2 and for length intratester 0.519 (0.097) cm and intertester 0.507 (0.085) cm. Conclusions: Extended field of view ultrasound imaging is an effective method of reliably capturing clear images of the TrA during rest and contraction. It provides an efficient mechanism for the analysis of TrA morphology by being able to measure the cross-sectional area, thickness, and length on one image. This methodology is recommended for studies investigating TrA function and training.

scholarly journals Extended field-of-view ultrasound imaging is reliable for measuring Transversus Abdominis muscle size at rest and during contraction

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
A. Wayne Johnson ◽  
Lauren Adams ◽  
Jade B. Kho ◽  
Daniel M. Green ◽  
Nicolas B. Pace ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Cross Sectional Area ◽  
Field Of View ◽  
Transversus Abdominis ◽  
Proper Function ◽  
Abdominal Muscles ◽  
Sectional Area ◽  
Cross Sectional ◽  
Extended Field ◽  
Extended Field Of View

Abstract Background The strength and size of core muscles, including the abdominal muscles, are crucial to proper function in most activities. Therefore, it is important to reliably assess these characteristics. Our primary objective was to determine if the length, thickness and cross-sectional area of the transversus abdominis (TrA) can be visualized independently from the internal and external abdominal oblique muscles using extended field of view ultrasound imaging at rest and with contraction and to establish its intra- and inter-tester reliability. Methods Twenty-six individuals were recruited to participate in the study (20 F, 6 M), average age 24.0 years (SD 9.4), height 170.7 cm (SD 8.6) and weight 63.9 kg (SD 9.0). From this total number of participants, two groups of 16 randomly selected participants were assessed to determine intra- and inter-tester reliability respectively. Extended field of view ultrasound images were obtained at three vertebral levels during rest and contraction in the side lying position for both the right and left sides of the trunk. Results Excellent intra-tester and inter-tester reliability was seen (ICC range of 0.972 to 0.984). The overall average percent standard error of the measurement for all measurements and locations was approximately 4%. The overall average minimal difference for the thickness measurement for the resting and contraction conditions combined were as follows: intratester 0.056 (0.014) cm and intertester 0.054 (0.017) cm, for area intratester 0.287 (0.086) cm2 and intertester 0.289 (0.101) cm2 and for length intratester 0.519 (0.097) cm and intertester 0.507 (0.085) cm. Conclusions Extended field of view ultrasound imaging is an effective method of reliably capturing clear images of the TrA during rest and contraction. It provides an efficient mechanism for the analysis of muscle morphology by being able to measure the cross-sectional area, thickness, and length on one image. This methodology is recommended for studies investigating TrA function and training.

Variability of Regional Quadriceps Architecture in Trained Men Assessed by B-Mode and Extended-Field-of-View Ultrasonography

2020 ◽  
Vol 15 (3) ◽  
pp. 430-436 ◽  
Author(s):  
Dustin J. Oranchuk ◽  
André R. Nelson ◽  
Adam G. Storey ◽  
John B. Cronin
Keyword(s):  
Vastus Lateralis ◽  
Intraclass Correlation ◽  
Muscle Thickness ◽  
Rectus Femoris ◽  
Field Of View ◽  
Fascicle Length ◽  
Large Variability ◽  
Extended Field ◽  
Extended Field Of View ◽  
Regional Muscle

Purpose: Regional muscle-architecture measures are reported widely; however, little is known about the variability of these measurements in the rectus femoris, vastus lateralis, and anterior and lateral vastus intermedius. The aim of this study was to quantify this variability. Methods: Regional muscle thickness, pennation angle (PA), and calculated and extended-field-of-view–derived fascicle length (FL) were quantified in 26 participants using ultrasonography across 51 limbs on 3 occasions. To quantify variability, the typical error of measurement (TEM) was multiplied by 2, and thresholds of 0.2–0.6 (small), 0.6–1.2 (moderate), 1.2–2.0 (large), 2.0–4.0 (very large), and >4.0 (extremely large) were applied. In addition, variability was deemed large when the intraclass correlation coefficient (ICC) was <.67 and coefficient of variation (CV) >10%, moderate when ICC > .67 or CV < 10% (but not both), and small when both ICC > .67 and CV < 10%. Results: Muscle thickness of all muscles and regions had low to moderate variability (ICC = .88–.98, CV = 2.4–9.3%, TEM = 0.15–0.47). PA of the proximal and distal vastus lateralis had low variability (ICC = .85–.96, CV = 3.8–8%) and moderate to large TEM (TEM = 0.42–0.83). PA of the rectus femoris was found to have moderate to very large variability (ICC = .38–.74, CV = 11.4–18.5%, TEM = 0.61–1.29) regardless of region. Extended-field-of-view–derived FL (ICC = .57–.94, CV = 4.1–11.5%, TEM = 0.26–0.88) was superior to calculated FL (ICC = .37–.84, CV = 7.4–17.9%, TEM = 0.44–1.33). Conclusions: Variability of muscle thickness was low in all quadriceps muscles and regions. Only rectus femoris PA and FL measurements were highly variable. The extended-field-of-view technique should be used to assess FL where possible. Inferences based on rectus femoris architecture should be interpreted with caution.

scholarly journals Test-Retest Reliability of Muscle Thickness, Echo-Intensity and Cross Sectional Area of Quadriceps and Hamstrings Muscle Groups Using B-mode Ultrasound

2017 ◽  
Vol 5 (1) ◽  
pp. 35 ◽  
Author(s):  
Cassio V. Ruas ◽  
Ronei S. Pinto ◽  
Camila D. Lima ◽  
Pablo B. Costa ◽  
Lee E. Brown
Keyword(s):  
Vastus Lateralis ◽  
Intraclass Correlation ◽  
Muscle Thickness ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Echo Intensity ◽  
Cross Sectional ◽  
Reliable Technique ◽  
Musculoskeletal Tissue ◽  
Muscle Groups

Ultrasound muscle images have been extensively used as tools for investigating, diagnosing and monitoring thigh muscles. However, there is a lack of information examining ultrasound reliability of quadriceps and hamstrings images for research and clinical use. Objectives: To determine the reliability of muscle thickness (MT), echo intensity (EI) and cross sectional area (CSA) of quadriceps and hamstrings muscle groups. Methods: Single transverse images of the rectus femoris (RF), vastus intermedius (VI), vastus medialis (VM), vastus lateralis (VL), biceps femoris long head (BFlh), semitendinosus (ST), and semimembranosus (SM) muscles were scanned in the right and left legs of ten healthy collegiate men (age 23.4 ± 2.2 yrs, mass 71.7 ± 11.7 kg, height 1.73 ± 0.06 m) between two sessions with one day interval. Intraclass correlation coefficients (ICCs), standard error of measurement (SEM), and minimum difference to be considered “real” (MD) were measured for MT, EI, and CSA. Results: A range of 0.97-0.99, 0.83-0.88, and 0.86-0.97 (ICC); 0.72-1.38, 2.73-3.41, and 0.36-1.04 (SEM); and 2.01-3.82, 7.56-9.46, and 0.99-2.89 (MD) were found for quadriceps muscles, and 0.93-0.99, 0.74-0.90, and 0.89-0.96 (ICC); 0.73-1.94, 3.29-4.98, and 0.69-1.08 (SEM); and 2.03-5.38, 9.13-13.81, and 1.91-2.98 (MD) were found for hamstrings muscles. Conclusions: These results suggest that ultrasound imaging of both quadriceps and hamstrings muscle architecture is a reliable technique for assessing thigh musculoskeletal tissue. The anatomical sites, as well as ultrasound adjustments, images, and results utilized here may assist future researchers and clinicians as reference tools when measuring quadriceps and hamstrings musculature. 

Regulation of fiber size, oxidative potential, and capillarization in human muscle by resistance exercise

1999 ◽  
Vol 276 (2) ◽  
pp. R591-R596 ◽  
Author(s):  
H. Green ◽  
C. Goreham ◽  
J. Ouyang ◽  
M. Ball-Burnett ◽  
D. Ranney
Keyword(s):  
Fiber Type ◽  
Vastus Lateralis ◽  
Succinic Dehydrogenase ◽  
Quadriceps Muscle ◽  
Cellular Tissue ◽  
Cross Sectional Area ◽  
Fiber Types ◽  
Sectional Area ◽  
Oxidative Potential ◽  
Cross Sectional

To examine the hypothesis that increases in fiber cross-sectional area mediated by high-resistance training (HRT) would result in a decrease in fiber capillarization and oxidative potential, regardless of fiber type, we studied six untrained males (maximum oxygen consumption, 45.6 ± 2.3 ml ⋅ kg−1 ⋅ min−1; mean ± SE) participating in a 12-wk program designed to produce a progressive hypertrophy of the quadriceps muscle. The training sessions, which were conducted 3 times/wk, consisted of three sets of three exercises, each performed for 6–8 repetitions maximum (RM). Measurements of fiber-type distribution obtained from tissue extracted from the vastus lateralis at 0, 4, 7, and 12 wk indicated reductions ( P < 0.05) in type IIB fibers (15.1 ± 2.1% vs. 7.2 ± 1.3%) by 4 wk in the absence of changes in the other fiber types (types I, IIA, and IIAB). Training culminated in a 17% increase ( P < 0.05) in cross-sectional area by 12 wk with initial increases observed at 4 wk. The increase was independent of fiber type-specific changes. The number of capillaries in contact with each fiber type increased by 12 wk, whereas capillary contacts-to-fiber area ratios remained unchanged. In a defined cross-sectional field, HRT also increased the capillaries per fiber at 12 wk. Training failed to alter cellular oxidative potential, as measured by succinic dehydrogenase (SDH) activity, regardless of fiber type and training duration. It is concluded that modest hypertrophy induced by HRT does not compromise cellular tissue capillarization and oxidative potential regardless of fiber type.

Effects of sprint cycle training on human skeletal muscle

1994 ◽  
Vol 77 (5) ◽  
pp. 2385-2390 ◽  
Author(s):  
C. A. Allemeier ◽  
A. C. Fry ◽  
P. Johnson ◽  
R. S. Hikida ◽  
F. C. Hagerman ◽  
...  
Keyword(s):  
Fiber Type ◽  
Vastus Lateralis ◽  
Cross Sectional Area ◽  
Aerobic Performance ◽  
Vastus Lateralis Muscle ◽  
Sectional Area ◽  
Cross Sectional ◽  
Fast Fiber ◽  
Cycle Training ◽  
Exercise Induced

Eleven men sprint trained two to three times per week for 6 wk to investigate possible exercise-induced slow-to-fast fiber type conversions. Six individuals served as controls. Both groups were tested at the beginning and end of the study to determine anaerobic performance and maximal oxygen consumption. In addition, pre- and postbiopsies were extracted from the vastus lateralis muscle and were analyzed for fiber type composition, cross-sectional area, and myosin heavy chain (MHC) content. No significant changes were found in anaerobic or aerobic performance variables for either group. Although a trend was found for a decrease in the percentage of type IIb fibers, high-intensity sprint cycle training caused no significant changes in the fiber type distribution or cross-sectional area. However, the training protocol did result in a significant decrease in MHC IIb with a concomitant increase in MHC IIa for the training men. These data appear to support previous investigations that have suggested exercise-induced adaptations within the fast fiber population (IIb-->IIa) after various types of training (endurance and strength).

The Effect of ACL Reconstruction on Involved and Contralateral Limb Vastus Lateralis Morphology and Histology: A Pilot Study

2019 ◽  
Author(s):  
Eric C. Leszczynski ◽  
Christopher Kuenze ◽  
Brett Brazier ◽  
Joseph Visker ◽  
David P. Ferguson
Keyword(s):  
Fiber Type ◽  
Vastus Lateralis ◽  
Knee Extension ◽  
Cross Sectional Area ◽  
Vastus Lateralis Muscle ◽  
Myosin Isoforms ◽  
Sectional Area ◽  
Cross Sectional ◽  
Contralateral Limb ◽  
Significant Difference

AbstractQuadriceps muscle weakness is a commonly reported issue post anterior cruciate ligament reconstruction (ACLR), with minimal information related to skeletal muscle morphology following surgery. The purpose is to examine the morphological and functional differences in the vastus lateralis muscle from patient's ACLR and contralateral leg. Three physically active ACLR participants were recruited and secured to a dynamometer to perform maximal voluntary isometric knee extension contractions (MVIC) of the ACLR and contralateral limb. Muscle biopsies of the ACLR and contralateral vastus lateralis were performed, then sectioned, and stained for myosin isoforms to determine fiber type. Confocal images were acquired, and ImageJ software was used to determine the fiber type and cross-sectional area (CSA). There was a significant reduction in CSA of the type IIa and type IIx muscle fiber cells between healthy (IIa: 7,718 ± 1,295 µm2; IIx; 5,800 ± 601 µm2) and ACLR legs (IIa: 4,139 ± 709 µm2; IIx: 3,708 ± 618 µm2) (p < 0.05), while there was no significant difference in knee extension MVIC torque between legs (healthy limb: 2.42 ± 0.52 Nm/kg; ACLR limb: 2.05 ± 0.24 Nm/kg, p = 0.11). The reduction in the cross-sectional area of the ACLR type II fibers could impair function and increase secondary injury risk.

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