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2022 ◽  
Vol 12 (5) ◽  
pp. 888-896
Author(s):  
Wenjuan Fan ◽  
Chen Xudong ◽  
Sun Yizheng ◽  
Shanshan Wu ◽  
Haili Wang ◽  
...  

Alzheimer’s disease (AD) is a progressive neurologic disorder that impacts a diverse population of older adults. As three-dimensional (3D) models are powerful tools for advancing AD studies, the authors have been developed AD cortical organoids to enable the observation of AD pathology at the cellular, tissue, and organ levels. For creating the model, APPSwe/Ind (APP) and PSEN1 (PS1) mutant genes were transfected into mouse induced pluripotent stem cells (iPSCs) following which the iPSC lines that expressed mutant APP and PS1 proteins were obtained. Then, using modified serum-free suspended embryoid body culture, AD cerebral organoids were made successfully at various ages. The AD model can show AD’s biochemical and pathological alterations, such as overexpressions of Aβ40 and Aβ42 and a decrease of GABAergic interneurons. The proposed model has the potential for implementation in many biomedical applications, including AD drug screening, stem cell transplant, and neuronal tissue engineering.


Author(s):  
Yongyu He ◽  
Wenqing Xie ◽  
Hengzhen Li ◽  
Hongfu Jin ◽  
Yi Zhang ◽  
...  

Aging promotes most degenerative pathologies in mammals, which are characterized by progressive decline of function at molecular, cellular, tissue, and organismal levels and account for a host of health care expenditures in both developing and developed nations. Sarcopenia is a prominent age-related disorder in musculoskeletal system. Defined as gradual and generalized chronic skeletal muscle disorder, sarcopenia involves accelerated loss of muscle mass, strength and function, which is associated with increased adverse functional outcomes and evolutionally refers to muscle wasting accompanied by other geriatric syndromes. More efforts have been made to clarify mechanisms underlying sarcopenia and new findings suggest that it may be feasible to delay age-related sarcopenia by modulating fundamental mechanisms such as cellular senescence. Cellular senescence refers to the essentially irreversible growth arrest mainly regulated by p53/p21CIP1 and p16INK4a/pRB pathways as organism ages, possibly detrimentally contributing to sarcopenia via muscle stem cells (MuSCs) dysfunction and the senescence-associated secretory phenotype (SASP) while cellular senescence may have beneficial functions in counteracting cancer progression, tissue regeneration and wound healing. By now diverse studies in mice and humans have established that targeting cellular senescence is a powerful strategy to alleviating sarcopenia. However, the mechanisms through which senescent cells contribute to sarcopenia progression need to be further researched. We review the possible mechanisms involved in muscle stem cells (MuSCs) dysfunction and the SASP resulting from cellular senescence, their associations with sarcopenia, current emerging therapeutic opportunities based on targeting cellular senescence relevant to sarcopenia, and potential paths to developing clinical interventions genetically or pharmacologically.


Author(s):  
Jacopo Umberto Verga ◽  
Matthew Huff ◽  
Diarmuid Owens ◽  
Bethany J. Wolf ◽  
Gary Hardiman

Exposure to Endocrine Disrupting Chemicals (EDC) has been linked with several adverse outcomes. In this review, we examine EDCs that are pervasive in the environment and are of concern in the context of human, animal, and environmental health. We explore the consequences of EDC exposure on aquatic life, terrestrial animals, and humans. We focus on the exploitation of genomics technologies and in particular whole transcriptome sequencing. Genome-wide analyses using RNAseq provides snap shots of cellular, tissue and whole organism transcriptomes under normal physiological and EDC perturbed conditions. A global view of gene expression provides highly valuable information as it uncovers gene families or more specifically, pathways that are affected by EDC exposures, but also reveals those that are unaffected. Hypotheses about genes with unknown functions can also be formed by comparison of their expression levels with genes of known function. Risk assessment strategies leveraging genomic technologies and the development of toxicology databases are explored. Finally, we review how the Adverse Outcome Pathway (AOP) has exploited this high throughput data to provide a framework for toxicology studies.


2021 ◽  
Author(s):  
Xuan Liang ◽  
Antonia Weberling ◽  
Chun Yuan Hii ◽  
Magdalena Zernicka-Goetz ◽  
Clare E Buckley

Individual cells within de novo polarising tubes and cavities must integrate their forming apical domains into a centralised apical membrane initiation site (AMIS). This is necessary to enable organised lumen formation within multi-cellular tissue. Despite the well documented importance of cell division in localising the AMIS, we have found a division-independent mechanism of AMIS localisation that relies instead on CADHERIN-mediated cell-cell adhesion. Our study of de novo polarising mouse embryonic stem cells (mESCs) cultured in 3D suggest that cell-cell adhesion directs the localisation of apical proteins such as PAR-6 to a centralised AMIS. Unexpectedly, we also found that mESC cell clusters lacking functional E-CADHERIN were still able to form a lumen-like cavity in the absence of AMIS localisation and did so at a later stage of development via a closure mechanism, instead of via hollowing. This work suggests that there are two, interrelated mechanisms of apical polarity localisation: cell adhesion and cell division. Alignment of these mechanisms in space allows for redundancy in the system and ensures the development of a coherent epithelial structure within a growing organ.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Stephanie Mohammed ◽  
Venkatesan Sundaram ◽  
Chalapathi R. Adidam Venkata ◽  
Nikolay Zyuzikov

Abstract Introduction The hypothalamic-pituitary-ovarian (HPO) axis is the principal regulator of the reproductive system. The neurons in the arcuate nucleus of the hypothalamus signal the basophilic cells of the anterior pituitary to release luteinizing hormone (LH) and follicle stimulating hormone (FSH), which bind to the granulosa and theca cells of a follicle in the ovary to promote healthy follicular development. Disruption of this process at any time can lead to polycystic ovaries and, if left untreated, can lead to Polycystic Ovarian Syndrome (PCOS), one of the leading causes of infertility. A novel treatment option using 150 kHz Intermediate Frequency (IF) Electromagnetic Radiation (EMR) has been proposed to monitor the effect of this frequency during cystic development. Methods To prove this, an experiment was conducted to study the effect of whole-body exposure to 150 kHz EMR for 8 weeks at receptor, cellular, tissue and hormonal levels on the HPO axis of 25 young cyclic female rats. Results The results showed that 150 kHz EMR did not affect the histoarchitecture of neurons of arcuate nucleus of the hypothalamus of PCO-induced rats. It was also found that the number of basophilic cells of the pituitary gland was increased and the immunoreactivity of LH and FSH secretion increased. This EMR field also decreased the development of follicular cysts in the ovary and possibly increased the immunoreactivity of the LH and FSH receptors as well on the theca and granulosa cells of follicles in the ovary. Conclusion There are still many limitations to this study. If properly evaluated, the results of this experiment could help develop a new non-invasive treatment option for women with PCOS in the near future.


Sensors ◽  
2021 ◽  
Vol 21 (22) ◽  
pp. 7614
Author(s):  
Natalia Rudenko ◽  
Ksenia Fursova ◽  
Anna Shepelyakovskaya ◽  
Anna Karatovskaya ◽  
Fedor Brovko

The recognition of biomolecules is crucial in key areas such as the timely diagnosis of somatic and infectious diseases, food quality control, and environmental monitoring. This determines the need to develop highly sensitive display devices based on the achievements of modern science and technology, characterized by high selectivity, high speed, low cost, availability, and small size. Such requirements are met by biosensor systems—devices for reagent-free analysis of compounds that consist of a biologically sensitive element (receptor), a transducer, and a working solution. The diversity of biological material and methods for its immobilization on the surface or in the volume of the transducer and the use of nanotechnologies have led to the appearance of an avalanche-like number of different biosensors, which, depending on the type of biologically sensitive element, can be divided into three groups: enzyme, affinity, and cellular/tissue. Affinity biosensors are one of the rapidly developing areas in immunoassay, where the key point is to register the formation of an antigen–antibody complex. This review analyzes the latest work by Russian researchers concerning the production of molecules used in various immunoassay formats as well as new fundamental scientific data obtained as a result of their use.


2021 ◽  
Vol 36 (5) ◽  
pp. 255-266
Author(s):  
Gennady A. Bocharov ◽  
Dmitry S. Grebennikov ◽  
Rostislav S. Savinkov

Abstract Pathophysiology of ischemic heart disease is a complex phenomenon determined by the interaction of multiple processes including the inflammatory, immunological, infectious, mechanical, biochemical and epigenetic ones. A predictive clinically relevant modelling of the entire trajectory of the human organism, from the initial alterations in lipid metabolism through to atherosclerotic plaque formation and finally to the pathologic state of the ischemic heart disease, is an open insufficiently explored problem. In the present review, we consider the existing mathematical frameworks which are used to describe, analyze and predict the dynamics of various processes related to cardiovascular diseases at the molecular, cellular, tissue, and holistic human organism level. The mechanistic, statistical and machine learning models are discussed in detail with special focus on the underlying assumptions and their clinical relevance. All together, they provide a solid computational platform for further expansion and tailoring for practical applications.


Children ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 945
Author(s):  
Mark S. Scher

Hypertensive disorders of pregnancy (HDP) contribute to adverse gene-environment interactions prior to conception and continue throughout pregnancy. Embryonic/fetal brain disorders occur from interactions between genetic susceptibilities interacting with acquired diseases or conditions affecting the maternal/placental fetal (MPF) triad. Trimester-specific pathophysiological mechanisms, such as maternal immune activation and ischemic placental syndrome, contribute to adverse peripartum, neonatal and childhood outcomes. Two diagnostic approaches provide timelier diagnoses over the first 1000 days from conception until two years of age. Horizontal analyses assess the maturation of the triad, neonate and child. Vertical analyses consider systems-biology from genetic, molecular, cellular, tissue through organ networks during each developmental niche. Disease expressions associated with HDP have cumulative adverse effects across the lifespan when subjected to subsequent adverse events. Critical/sensitive periods of developmental neuroplasticity over the first 1000 days are more likely to result in permanent sequelae. Novel diagnostic approaches, beginning during pre-conception, will facilitate the development of effective preventive, rescue and reparative neurotherapeutic strategies in response to HDP-related trimester-specific disease pathways. Public health policies require the inclusion of women’s health advocacy during and beyond their reproductive years to reduce sequelae experienced by mothers and their offspring. A lower global burden of neurologic disease from HDP will benefit future generations.


2021 ◽  
Author(s):  
Jian Zhou ◽  
Junpeng Lai ◽  
Gil Menda ◽  
Jay A. Stafstrom ◽  
Carol I. Miles ◽  
...  

Hearing is a fundamental sense of many animals, including all mammals, birds, some reptiles, amphibians, fish and arthropods1,2. The auditory organs of these animals are extremely diverse in anatomy after hundreds of millions of years of evolution3-5, yet all are made up of cellular tissue and are embodied meaning that its functional anatomy is constrained by developmental morphogenesis. Here we show hearing in the orb-weaving spider, Larinioides sclopetarius is not constrained by embodiment but is extended through outsourcing hearing to its proteinaceous, self-manufactured orb-web, and hence under behavioral control, not developmental biology. We find the wispy, wheel-shaped orb-web acts as a hyperacute acoustic array to capture the sound-induced air particle movements that approach the maximum physical efficiency, better than the acoustic responsivity of all previously known ears6,7. By manipulating the web threads with its eight vibration-sensitive legs8-10, the spider remotely detects and localizes the source of an incoming airborne acoustic wave emitted by approaching prey or predators. By outsourcing its acoustic sensors to its web, the spider is released from embodied morphogenetic constraints and permits the araneid spider to increase its sound-sensitive surface area enormously, up to 10,000 times greater than the spider itself11. The use of the web also enables a spider the flexibility to functionally adjust and regularly regenerate its 'external ear' according to its needs. This finding opens a new perspective on animal hearing - the 'outsourcing' and 'supersizing' of auditory function in a spider, one of the earliest animals to live on land12. The novel hearing mechanism provides unique features for studying extended and regenerative sensing13-15, and designing novel acoustic flow detectors for precise fluid dynamic measurement and manipulation16-18.


2021 ◽  
pp. 161-196
Author(s):  
Nathan Denton

The associations between overall and regional fat mass with numerous health outcomes seem to transcend age, sex, and ethnicity, suggesting that they are underpinned by some common mechanisms. This chapter aims to make sense of these striking relationships by considering the macroscopic, cellular, and molecular processes operating within various anatomically distinct fat depots to help rationalize the complex relationship between regional fat accumulation and various aspects of metabolic, cardiovascular, and reproductive health. After outlining how fat depots around the body behave differently, the following section examines why this may be the case. This involves considering various phenomena, namely adipogenesis, gene expression, and epigenetics, that underpin the biological properties of adipocytes which aggregate to inform the behaviour of fat as a multi-cellular tissue.


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