Ultrafast two-photon microscopy for high-speed brain imaging in awake mice (Conference Presentation)

2018 ◽  
Author(s):  
Radoslaw Chrapkiewicz ◽  
Tong Zhang ◽  
Oscar Hernandez ◽  
Adam S. Shai ◽  
Mark J. Wagner ◽  
...  
Keyword(s):  
Brain Imaging ◽  
High Speed ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Publisher Correction: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

2021 ◽  
Vol 18 (2) ◽  
pp. 220-220
Author(s):  
Weijian Zong ◽  
Runlong Wu ◽  
Shiyuan Chen ◽  
Junjie Wu ◽  
Hanbin Wang ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Field Of View ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals The Power of Single and Multibeam Two-Photon Microscopy for High-Resolution and High-Speed Deep Tissue and Intravital Imaging

2007 ◽  
Vol 93 (7) ◽  
pp. 2519-2529 ◽  
Author(s):  
Raluca Niesner ◽  
Volker Andresen ◽  
Jens Neumann ◽  
Heinrich Spiecker ◽  
Matthias Gunzer
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Intravital Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals High speed functional imaging with source localized multifocal two-photon microscopy

2018 ◽  
Vol 9 (8) ◽  
pp. 3678 ◽  
Author(s):  
Peter Quicke ◽  
Stephanie Reynolds ◽  
Mark Neil ◽  
Thomas Knöpfel ◽  
Simon R. Schultz ◽  
...  
Keyword(s):  
Functional Imaging ◽  
High Speed ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Kilohertz frame-rate two-photon tomography

2018 ◽  
Author(s):  
Abbas Kazemipour ◽  
Ondrej Novak ◽  
Daniel Flickinger ◽  
Jonathan S. Marvin ◽  
Jonathan King ◽  
...  
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Glutamate Release ◽  
Single Particle Tracking ◽  
Frame Rate ◽  
Mammalian Brain ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Calcium Sensors ◽  
Structural Image

SummaryPoint-scanning two-photon microscopy enables high-resolution imaging within scattering specimens such as the mammalian brain, but sequential acquisition of voxels fundamentally limits imaging speed. We developed a two-photon imaging technique that scans lines of excitation across a focal plane at multiple angles and uses prior information to recover high-resolution images at over 1.4 billion voxels per second. Using a structural image as a prior for recording neural activity, we imaged visually-evoked and spontaneous glutamate release across hundreds of dendritic spines in mice at depths over 250 µm and frame-rates over 1 kHz. Dendritic glutamate transients in anaesthetized mice are synchronized within spatially-contiguous domains spanning tens of microns at frequencies ranging from 1-100 Hz. We demonstrate high-speed recording of acetylcholine and calcium sensors, 3D single-particle tracking, and imaging in densely-labeled cortex. Our method surpasses limits on the speed of raster-scanned imaging imposed by fluorescence lifetime.

scholarly journals Fast objective coupled planar illumination microscopy

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Cody J. Greer ◽  
Timothy E. Holy
Keyword(s):  
Fluorescence Microscopy ◽  
High Speed ◽  
Imaging Techniques ◽  
Light Sheet ◽  
Three Dimensions ◽  
Planar Imaging ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Larval Zebrafish ◽  
Fast Light

Abstract Among optical imaging techniques light sheet fluorescence microscopy is one of the most attractive for capturing high-speed biological dynamics unfolding in three dimensions. The technique is potentially millions of times faster than point-scanning techniques such as two-photon microscopy. However light sheet microscopes are limited by volume scanning rate and/or camera speed. We present speed-optimized Objective Coupled Planar Illumination (OCPI) microscopy, a fast light sheet technique that avoids compromising image quality or photon efficiency. Our fast scan system supports 40 Hz imaging of 700 μm-thick volumes if camera speed is sufficient. We also address the camera speed limitation by introducing Distributed Planar Imaging (DPI), a scaleable technique that parallelizes image acquisition across cameras. Finally, we demonstrate fast calcium imaging of the larval zebrafish brain and find a heartbeat-induced artifact, removable when the imaging rate exceeds 15 Hz. These advances extend the reach of fluorescence microscopy for monitoring fast processes in large volumes.

scholarly journals Photon counting, censor corrections, and lifetime imaging for improved detection in two-photon microscopy

2011 ◽  
Vol 105 (6) ◽  
pp. 3106-3113 ◽  
Author(s):  
Jonathan D. Driscoll ◽  
Andy Y. Shih ◽  
Satish Iyengar ◽  
Jeffrey J. Field ◽  
G. Allen White ◽  
...  
Keyword(s):  
High Speed ◽  
Signal To Noise Ratio ◽  
Photon Counting ◽  
In Vivo Model ◽  
Fluorescence Lifetime Imaging ◽  
Photon Counter ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Lifetime Imaging

We present a high-speed photon counter for use with two-photon microscopy. Counting pulses of photocurrent, as opposed to analog integration, maximizes the signal-to-noise ratio so long as the uncertainty in the count does not exceed the gain-noise of the photodetector. Our system extends this improvement through an estimate of the count that corrects for the censored period after detection of an emission event. The same system can be rapidly reconfigured in software for fluorescence lifetime imaging, which we illustrate by distinguishing between two spectrally similar fluorophores in an in vivo model of microstroke.

scholarly journals Dual GRIN lens two-photon endoscopy for high-speed volumetric and deep brain imaging

2020 ◽  
Vol 12 (1) ◽  
pp. 162
Author(s):  
Yu-Feng Chien ◽  
Jyun-Yi Lin ◽  
Po-Ting Yeh ◽  
Kuo-Jen Hsu ◽  
Yu-Hsuan Tsai ◽  
...  
Keyword(s):  
Brain Imaging ◽  
High Speed ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

Remote focusing system for simultaneous dual-plane mesoscopic multiphoton imaging

2018 ◽  
Author(s):  
Dmitri Tsyboulski ◽  
Natalia Orlova ◽  
Fiona Griffin ◽  
Sam Seid ◽  
Jerome Lecoq ◽  
...  
Keyword(s):  
High Speed ◽  
Imaging System ◽  
Fold Increase ◽  
Large Field ◽  
Multiphoton Imaging ◽  
Dual Plane ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Analog Signals ◽  
Multiplexed Imaging

We present a dual-plane mesoscopic imaging system capable of simultaneous image acquisition from two independent focal planes. The system was designed as an add-on to a recently introduced large field-of-view two-photon microscopy system, developed by Sofroniew, et al., eLife, 5, e14472, 2016. In this work, we merge two advanced multiphoton imaging technologies, i.e., temporal-division multiplexing and remote focusing, to maintain diffraction-limited resolution at both imaging planes, and achieve a more than 2-fold increase in the system's overall imaging throughput. We introduce a novel solution to decode temporally interleaved analog signals at nanosecond timescales to achieve high-speed time-multiplexed imaging. Detailed characterization and comparison of the modified and the original two-photon microscopy system was performed.

scholarly journals Toward multi-focal spot remote focusing two-photon microscopy for high speed imaging

2017 ◽  
Author(s):  
Bei Li ◽  
Alexander D. Corbett ◽  
Ee Chong ◽  
Edward Mann ◽  
Tony Wilson ◽  
...  
Keyword(s):  
High Speed ◽  
Focal Spot ◽  
High Speed Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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