Quantitative characterization technique for assessment of malignancy in breast fine needle aspirations

The surface roughness (Ra) and composite interfacial property of carbon fiber (CF) are considered to be mainly affected by the microstructure of the CF surface. However, quantitative characterization of the CF surface microstructure is always a difficulty. How the CF surface microstructure affects the interfacial property of CF composites is not entirely clear. A quantitative characterization technique based on images was established to calculate the cross-section perimeter and area of five types of CFs, as well as the number (N), width (W) and depth (D) of grooves on these CF surfaces. The CF composite interfacial shear strength (IFSS) was tested by the micro-droplet debonding test and modified by the realistic perimeter. The relationship between the groove structure parameter and the Ra, specific surface area and composite interfacial property was discussed in this article. The results indicated that the CF cross-section perimeter calculated by this technique showed strong consistency with the CF specific surface area and composite interfacial property. At last, the composite interface bonding mechanism based on defect capture was put forward. This mechanism can be a guiding principle for CF surface modification and help researchers better understand and establish interface bonding theories.

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Ultrastructural diagnosis of plasmacytoma on a fine-needle aspirate

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100084818 ◽

1991 ◽

Vol 49 ◽

pp. 102-103

Author(s):

S. Siew ◽

W. deMendonca-Calaca

Keyword(s):

Mononuclear Cells ◽

Fibrous Tissue ◽

Needle Aspiration ◽

Focal Lesions ◽

Diffuse Infiltration ◽

Fine Needle ◽

Cytoplasmic Vacuolation ◽

Chest Mass ◽

Mitotic Figures ◽

Microscopy Examination

A 36 year old man presented with a mass in the chest and multiple “hot” focal lesions were identified on bone scan. Fine needle aspiration was performed of the chest mass. Routine histology showed the presence of some bundles of dense fibrous tissue and a diffuse infiltration of mononuclear cells, which varied in size and nucleo-cytoplasmic ratio. The smaller cells had eccentric hyperchromatic nuclei. Nucleoli were noted in the larger cells. There was well marked cytoplasmic vacuolation of some of the cells. Mitosis was present. A small fragment of tissue was received for electron microscopy. Examination of 1 μm sections showed trabeculae of medium-large polygonal cells with eccentric nuclei and occasional nucleoli. Some irregularly shaped cells had well marked cytoplasmic vacuolation. Mitotic figures were present.

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Aspiration cytology and transmission electron microscopy (TEM): A novel tissue-collection technique with useful diagnostic applications

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100110751 ◽

1984 ◽

Vol 42 ◽

pp. 90-93

Author(s):

J.A. Maksem ◽

C. VanDyke ◽

H.W. Carter ◽

C.F. Galang

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Diagnostic Technique ◽

Needle Aspiration ◽

Large Series ◽

Aspiration Cytology ◽

Fine Needle ◽

Transmission Electron ◽

Diagnostic Applications ◽

Microscopic Slide

In the last decade fine needle aspiraration biopsy has gained recognition as a valuable diagnostic technique, and its benefits have been demonstrated in large series of patients with almost every type of tumor (1,2). The usual way to collect cellular material from needle-aspiration biopsies is to discharge the needle and syringe contents onto a microscopic slide and smear the material with another slide. The entire specimen is contained on the slides prepared at the time of biopsy. Serious technical difficulties are inherent to this method. 1) Inconsistent fixation, 2) drying artifact, 3) loss of tissue fragments, 4) inability to confirm impressions by a “second method”, and 5) retention of significant diagnostic material in the needle hub. Our technique avoids these difficulties.

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