Photoacoustic imaging of fear conditioning neuronal ensembles using a Fos-Lacz gene reporter system in the rat brain in vivo after intrathecal X-gal injection

AbstractThe formation and extinction of fear memories represent two forms of learning that each engage the hippocampus and amygdala. How cell populations in these areas contribute to fear relapse, however, remains unclear. Here, we demonstrate that, in mice, cells active during fear conditioning in the dentate gyrus of hippocampus and basolateral amygdala exhibit decreased activity during extinction and are re-engaged after fear reinstatement. In vivo calcium imaging reveals that reinstatement drives population dynamics in the basolateral amygdala to revert to a network state similar to the state present during fear conditioning. Finally, we find that optogenetic inactivation of neuronal ensembles active during fear conditioning in either the hippocampus or amygdala is sufficient to disrupt fear expression after reinstatement. These results suggest that fear reinstatement triggers a partial re-emergence of the original fear memory representation, providing new insight into the neural substrates of fear relapse.

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A UCP1 Near-Infrared Reporter System for Monitoring Adipose “Browning” In Vivo Using Photoacoustic Imaging

Diabetes ◽

10.2337/db18-280-or ◽

2018 ◽

Vol 67 (Supplement 1) ◽

pp. 280-OR

Author(s):

DERRYN XIN HUI CHAN ◽

SHIGEKI SUGII ◽

WEIPING HAN

Keyword(s):

Near Infrared ◽

Photoacoustic Imaging ◽

Reporter System

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Photoacoustic imaging of lacZ gene expression in vivo

Journal of Biomedical Optics ◽

10.1117/1.2717531 ◽

2007 ◽

Vol 12 (2) ◽

pp. 020504 ◽

Cited By ~ 117

Author(s):

Li Li ◽

Roger J. Zemp ◽

Gina Lungu ◽

George Stoica ◽

Lihong V. Wang

Keyword(s):

Gene Expression ◽

Photoacoustic Imaging ◽

Lacz Gene

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A human photoacoustic imaging reporter gene using the clinical dye indocyanine green

10.1101/537100 ◽

2019 ◽

Author(s):

Nivin N. Nyström ◽

Lawrence C.M. Yip ◽

Jeffrey J.L. Carson ◽

Timothy J. Scholl ◽

John A. Ronald

Keyword(s):

Indocyanine Green ◽

Cell Tracking ◽

Photoacoustic Imaging ◽

Organic Anion ◽

Reporter System ◽

Optical Contrast ◽

Organic Anion Transporting Polypeptide ◽

Gene Therapies

ABSTRACTPhotoacoustic imaging (PAI) combines optical contrast with the resolution and depth-detection of ultrasound and is increasingly being utilized for medical imaging in patients. PAI reporter genes would allow for monitoring of cell and gene therapies, but current reporters have immunogenicity and/or toxicity concerns that may limit clinical translation. Here we report a PAI reporter system employing the ability of human organic anion transporting polypeptide 1b3 (Oatp1b3) to take up the clinical dye indocyanine green (ICG) into cells. Following ICG administration, cells synthetically expressing Oatp1b3 exhibited significantly increased PAI signals compared to control cells both in vitro and in mice. Several benefits of this technology are the human derivation of Oatp1b3, and the high extinction coefficient, low quantum yield and pre-existing clinical approval of ICG. We posit that the Oatp1b3-ICG reporter system could be useful for in vivo gene and cell tracking in preclinical and clinical applications.

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