Lithocell availability improvement through light source maintenance cycle improvement and optimization and its availability impact analysis for cutting-edge ArFi light source

The output of the ultramicrotomy process with its high strain levels is dependent upon the input, ie., the nature of the material being machined. Apart from the geometrical constraints offered by the rake and clearance faces of the tool, each material is free to deform in whatever manner necessary to satisfy its material structure and interatomic constraints. Noncrystalline materials appear to survive the process undamaged when observed in the TEM. As has been demonstrated however microtomed plastics do in fact suffer damage to the top and bottom surfaces of the section regardless of the sharpness of the cutting edge or the tool material. The energy required to seperate the section from the block is not easily propogated through the section because the material is amorphous in nature and has no preferred crystalline planes upon which defects can move large distances to relieve the applied stress. Thus, the cutting stresses are supported elastically in the internal or bulk and plastically in the surfaces. The elastic strain can be recovered while the plastic strain is not reversible and will remain in the section after cutting is complete.

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A Method for Setting the Clearance Angle

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100095200 ◽

1972 ◽

Vol 30 ◽

pp. 388-389

Author(s):

Michael T. Bucek ◽

Howard J. Arnott

Keyword(s):

Experimental Evidence ◽

Light Source ◽

Black Spot ◽

Critical Factor ◽

Clearance Angle ◽

Crude Approximation ◽

Ultrathin Sections

It is believed by the authors, with supporting experimental evidence, that as little as 0.5°, or less, knife clearance angle may be a critical factor in obtaining optimum quality ultrathin sections. The degree increments located on the knife holder provides the investigator with only a crude approximation of the angle at which the holder is set. With the increments displayed on the holder one cannot set the clearance angle precisely and reproducibly. The ability to routinely set this angle precisely and without difficulty would obviously be of great assistance to the operator. A device has been contrived to aid the investigator in precisely setting the clearance angle. This device is relatively simple and is easily constructed. It consists of a light source and an optically flat, front surfaced mirror with a minute black spot in the center. The mirror is affixed to the knife by placing it permanently on top of the knife holder.

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A confocal laser scanning microscope for fluorescence and reflection at video rates

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100152641 ◽

1989 ◽

Vol 47 ◽

pp. 134-135

Author(s):

P.M. Houpt ◽

A. Draaijer

Keyword(s):

Light Source ◽

Laser Scanning ◽

Focal Point ◽

Confocal Laser Scanning Microscope ◽

Confocal Laser ◽

Point Light Source ◽

Volume Of Interest ◽

Ion Laser ◽

Point Light ◽

Point Detector

In confocal microscopy, the object is scanned by the coinciding focal points (confocal) of a point light source and a point detector both focused on a certain plane in the object. Only light coming from the focal point is detected and, even more important, out-of-focus light is rejected.This makes it possible to slice up optically the ‘volume of interest’ in the object by moving it axially while scanning the focused point light source (X-Y) laterally. The successive confocal sections can be stored in a computer and used to reconstruct the object in a 3D image display.The instrument described is able to scan the object laterally with an Ar ion laser (488 nm) at video rates. The image of one confocal section of an object can be displayed within 40 milliseconds (1000 х 1000 pixels). The time to record the total information within the ‘volume of interest’ normally depends on the number of slices needed to cover it, but rarely exceeds a few seconds.

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Expert Recommends Using This Year's Cutting-Edge Billing Codes

Pediatric News ◽

10.1016/s0031-398x(06)71125-0 ◽

2006 ◽

Vol 40 (6) ◽

pp. 42

Author(s):

KERRI WACHTER

Keyword(s):

Cutting Edge

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Phonon Physics The Cutting Edge

10.1016/s1874-5628(06)x8011-7 ◽

1995 ◽

Keyword(s):

Cutting Edge

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Inter-Method Reliability of Pulse Volume Related Measures Derived Using Finger-Photoplethysmography

Journal of Psychophysiology ◽

10.1027/0269-8803/a000197 ◽

2018 ◽

Vol 32 (4) ◽

pp. 182-190 ◽

Cited By ~ 4

Author(s):

Kenta Matsumura ◽

Koichi Shimizu ◽

Peter Rolfe ◽

Masanori Kakimoto ◽

Takehiro Yamakoshi

Keyword(s):

Light Intensity ◽

Adverse Effects ◽

Light Source ◽

Direct Current ◽

Resting State ◽

Current Component ◽

Psychophysiological Measures ◽

Pulse Volume ◽

Optical Paths ◽

Sensor Positions

Abstract. Pulse volume (PV) and its related measures, such as modified normalized pulse volume (mNPV), direct-current component (DC), and pulse rate (PR), derived from the finger-photoplethysmogram (FPPG), are useful psychophysiological measures. Although considerable uncertainties exist in finger-photoplethysmography, little is known about the extent of the adverse effects on the measures. In this study, we therefore examined the inter-method reliability of each index across sensor positions and light intensities, which are major disturbance factors of FPPG. From the tips of the index fingers of 12 participants in a resting state, three simultaneous FPPGs having overlapping optical paths were recorded, with their light intensity being changed in three steps. The analysis revealed that the minimum values of three coefficients of Cronbach’s α for ln PV, ln mNPV, ln DC, and PR across positions were .948, .850, .922, and 1.000, respectively, and that those across intensities were .774, .985, .485, and .998, respectively. These findings suggest that ln mNPV and PR can be used for psychophysiological studies irrespective of minor differences in sensor attachment positions and light source intensity, whereas and ln DC can also be used for such studies but under the condition of light intensity being fixed.

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