High-speed high-resolution epifluorescence imaging system using CCD sensor and digital storage for neurobiological research

2001 ◽  
Author(s):  
Ichiro Takashima ◽  
Riichi Kajiwara ◽  
Kiyo Murano ◽  
Toshio Iijima ◽  
Yasuhiro Morinaka ◽  
...  
2008 ◽  
Vol 55 (3) ◽  
pp. 1241-1243 ◽  
Author(s):  
D.W. Evertson ◽  
M.R. Holcomb ◽  
M.D.C. Eames ◽  
M.-A. Bray ◽  
V.Y. Sidorov ◽  
...  

1999 ◽  
Vol 5 (S2) ◽  
pp. 364-365
Author(s):  
G.Y. Fan ◽  
S. Peltier ◽  
S. Lamont ◽  
S.J. Young ◽  
Dana G. Dunkelberger ◽  
...  

A multiport-readout, frame-transfer charge coupled device (CCD) digital imaging system has been successfully constructed and tested for intermediate-high voltage electron microscopy (IVEM) applications up to 400 keV. The system employs a back-thinned CCD, made by MIT Lincoln laboratories, that comprises 2560 × 1960 pixels and a pixel size of 24 μm × 24 μm. With a frame transfer design, the imager fills nearly the entire usable area of a 100 mm-diameter silicon wafer (FIG. 1). In the current implementation, four of the eight on-chip readout ports are utilized in parallel each operating at a pixel rate of 1 or 2 MHz so that the entire CCD array can be read out in as short as ∼0.6 seconds. The frame-transfer readout functions as an electronic shutter which permits the rapid transfer of charges in the active pixels to four light-shielded buffers (FIG. 1) where the charges are readout and digitized while the active area of the CCD is integrating the next frame. At 2 MHz, charge transfer of 980 CCD rows will be completed in under 0.5 ms, which is much shorter than a typical exposure time of a few seconds. The camera head (FIG.2) and control electronics for CCD sensor were packaged by Photometries (Tuscon, Arizona). Two MaxVideo 200 image processing boards from Datacube (Peabody, MA) are used for high speed online imaging processing. The CCD sensor is electronically cooled to ∼ −40°C during operation.


2020 ◽  
Vol 142 (9) ◽  
Author(s):  
Ken Maassen ◽  
Farzad Poursadegh ◽  
Caroline Genzale

Abstract Modern high-efficiency engines utilize direct injection for charge preparation at extremely high pressures. At these conditions, the scales of atomization become challenging to measure, as primary breakup occurs on the micrometer and nanosecond scales. As such, fuel sprays at these conditions have proven difficult to study via direct imaging. While high-speed cameras now exist that can shutter at tens to hundreds of nanoseconds, and long-range microscopes can be coupled to these cameras to provide high-resolution images, the resolving power of these systems is typically limited by pixel size and field of view (FOV). The large pixel sizes make the realization of the diffraction-limited optical resolution quite challenging. On the other hand, limited data throughput under high repetition rate operation limits the FOV due to reduced sensor area. Therefore, a novel measurement technique is critical to study fuel spray formation at engine-relevant conditions. In this work, we demonstrate a new high-resolution imaging technique, spectral microscopy, which aims to realize diffraction-limited imaging at effective framerates sufficient for capturing primary breakup in engine-relevant sprays. A spectral microscopy system utilizing a consumer-grade DSLR allows for significantly wider FOV with improved resolving power compared to high-speed cameras. Temporal shuttering is accomplished via separate and independently triggered back illumination sources, with wavelengths selected to overlap with the detection bands of the camera sensor's RGB filter array. The RGB detection channels act as filters to capture independently timed red, green, and blue light pulses, enabling the capture of a three consecutive images at effective framerates exceeding 20 × 106 fps. To optimize system performance, a backlit illumination system is designed to maximize light throughput, a multilens setup is created, and an image-processing algorithm is demonstrated that formulates a three-frame image from the camera sensor. The system capabilities are then demonstrated by imaging engine relevant diesel sprays. The spectral microscopy system detailed in this paper allows for micron-scale feature recognition at framerates exceeding 20 × 106 fps, thus expanding the capability for experimental research on primary breakup in fuel sprays for modern direct-injection engines.


2021 ◽  
Author(s):  
Zhengyuan Zhu ◽  
Shaowen Peng ◽  
Yirong Xu ◽  
Xiaoping Zheng

Author(s):  
Kenneth Krieg ◽  
Richard Qi ◽  
Douglas Thomson ◽  
Greg Bridges

Abstract A contact probing system for surface imaging and real-time signal measurement of deep sub-micron integrated circuits is discussed. The probe fits on a standard probe-station and utilizes a conductive atomic force microscope tip to rapidly measure the surface topography and acquire real-time highfrequency signals from features as small as 0.18 micron. The micromachined probe structure minimizes parasitic coupling and the probe achieves a bandwidth greater than 3 GHz, with a capacitive loading of less than 120 fF. High-resolution images of submicron structures and waveforms acquired from high-speed devices are presented.


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