We demonstrated a scanning radially-polarized surface plasmon microscopy (SSPM) with submicron lateral resolution and high refractive index sensitivity. According to the captured images, SSPM could be used to verify the quality of deposited Au film and Au structure on surface plasmon sensing chip. The defect on the SPR chip, which disturbed the plasmonic wave around the focal point, could be easily detected by our system. Moreover, the application of SSPM in imaging lipid array was showed in this article. The 1, 2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) array was made by dip-pen nanolithography. The results indicated that SSPM system could be used in comparing the thickness of DOPC array. Because of the high sensitivity and high spatial resolution of the system, we expected the SSPM to be used in measuring the properties of nanogold array structures and apply to digital-array biosensors in nanoscale.
Time-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam
