Photoacoustic imaging of chemotherapy-induced apoptosis in squamous cell carcinoma

Isoliquiritigenin is a flavonoid commonly found in liquorice and has been identified as a potent anti-tumor agent. The aim of this study was to investigate whether isoliquiritigenin regulates the proliferation and apoptosis of tongue squamous cell carcinoma cells by regulating forkhead box G1 expression via miR-21. MTT assay and flow cytometry were used to analyze cell proliferation and apoptosis, respectively. Quantitative real time polymerase chain reaction and western blotting were used to detect mRNA and protein expression levels, respectively. The relationship between miR-21 and forkhead box G1 was detected by dual luciferase assay. Isoliquiritigenin inhibited proliferation and induced apoptosis of tongue squamous cell carcinoma cells, and decreased miR-21 levels and promoted forkhead box G1 expression. Forkhead box G1 was then identified as a target of miR-21 and ISL could promote forkhead box G1 expression by inhibiting miR-21. Further analysis suggested that upregulation of miR-21 improved proliferation and suppressed apoptosis of tongue squamous cell carcinoma cells by inhibiting forkhead box G1 expression. Finally, our results revealed that isoliquiritigenin inhibited proliferation and induced apoptosis of tongue squamous cell carcinoma cells by regulating miR-21. Isoliquiritigenin might act as a novel therapeutic treatment for tongue squamous cell carcinoma cells through up-regulation of forkhead box G1 expression via inhibiting miR-21expression.

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Periplocin Sensitizes Human Esophageal Squamous Cell Carcinoma to Trail-Induced Apoptosis via Upregulating Death Receptors and Inhibiting the Wnt/β-Catenin Pathway

SSRN Electronic Journal ◽

10.2139/ssrn.3437785 ◽

2019 ◽

Author(s):

Lujuan Han ◽

Suli Dai ◽

Zhirong Li ◽

Cong Zhang ◽

Sisi Wei ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Death Receptors ◽

Induced Apoptosis

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SDF-1-loaded PLGA nanoparticles for the targeted photoacoustic imaging and photothermal therapy of metastatic lymph nodes in tongue squamous cell carcinoma

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2018.10.064 ◽

2019 ◽

Vol 554 ◽

pp. 93-104 ◽

Cited By ~ 5

Author(s):

Jun Xiong ◽

JiaLi Feng ◽

Lihua Qiu ◽

Zhi Gao ◽

Pan Li ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Nodes ◽

Cell Carcinoma ◽

Squamous Cell ◽

Photothermal Therapy ◽

Photoacoustic Imaging ◽

Plga Nanoparticles ◽

Tongue Squamous Cell Carcinoma ◽

Metastatic Lymph Nodes ◽

Metastatic Lymph

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Antitumor Activity of the Cardiac Glycoside αlDiginoside by Modulating Mcl-1 in Human Oral Squamous Cell Carcinoma Cells

International Journal of Molecular Sciences ◽

10.3390/ijms21217947 ◽

2020 ◽

Vol 21 (21) ◽

pp. 7947

Author(s):

Jing-Ru Weng ◽

Wei-Yu Lin ◽

Li-Yuan Bai ◽

Jing-Lan Hu ◽

Chia-Hsien Feng

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Cardiac Glycoside ◽

Janus Kinase ◽

Parp Cleavage ◽

Diphenyltetrazolium Bromide ◽

Indigenous Plant ◽

Induced Apoptosis

We recently isolated a cardiac glycoside (CG), αldiginoside, from an indigenous plant in Taiwan, which exhibits potent tumor-suppressive efficacy in oral squamous cell carcinoma (OSCC) cell lines (SCC2095 and SCC4, IC50 < 0.2 µM; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays). Here, we report that αldiginoside caused Sphase arrest and apoptosis, through the inhibition of a series of signaling pathways, including those mediated by cyclin E, phospho-CDC25C (p-CDC25C), and janus kinase/signal transducer and activator of transcription (JAK/STAT)3. αldiginoside induced apoptosis, as indicated by caspase activation and poly (ADP-ribose) polymerase (PARP) cleavage. Equally important, αldiginoside reduced Mcl-1 expression through protein degradation, and overexpression of Mcl-1 partially protected SCC2095 cells from αldiginoside’s cytotoxicity. Taken together, these data suggest the translational potential of αldiginoside to foster new therapeutic strategies for OSCC treatment.

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