scholarly journals Electrophysiological audiograms in seven beluga whales (Delphinapterus leucas) from the Okhotsk Sea population

2018 ◽  
Author(s):  
Evgeniya V. Sysueva ◽  
Dmitry I. Nechaev ◽  
Vladimir V. Popov ◽  
Alexander Ya. Supin
Keyword(s):  
Delphinapterus Leucas ◽  
Okhotsk Sea ◽  
Beluga Whales

Binaural AEP audiograms in seven Beluga whales (Delphinapterus leucas) from the Okhotsk Sea population

2018 ◽  
Vol 143 (3) ◽  
pp. 1728-1728
Author(s):  
Evgeniya Sysueva ◽  
Dmitry Nechaev ◽  
Vladimir Popov ◽  
Mikhail Tarakanov ◽  
Alexander Supin
Keyword(s):  
Delphinapterus Leucas ◽  
Okhotsk Sea ◽  
Beluga Whales

Teeth as biomonitors of selenium concentrations in tissues of beluga whales (Delphinapterus leucas)

2008 ◽  
Vol 402 (1) ◽  
pp. 43-50 ◽  
Author(s):  
April Kinghorn ◽  
Murray M. Humphries ◽  
Peter Outridge ◽  
Hing Man Chan
Keyword(s):  
Delphinapterus Leucas ◽  
Beluga Whales

Vitamin A and E profiles as biomarkers of PCB exposure in beluga whales (Delphinapterus leucas) from the western Canadian Arctic

2013 ◽  
Vol 142-143 ◽  
pp. 317-328 ◽  
Author(s):  
Jean-Pierre W. Desforges ◽  
Peter S. Ross ◽  
Neil Dangerfield ◽  
Vince P. Palace ◽  
Michael Whiticar ◽  
...  
Keyword(s):  
Vitamin A ◽  
Canadian Arctic ◽  
Delphinapterus Leucas ◽  
Beluga Whales ◽  
Pcb Exposure

scholarly journals A probe-based qRT-PCR method to profile immunological gene expression in blood of captive beluga whales (Delphinapterus leucas)

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3840 ◽  
Author(s):  
Ming-An Tsai ◽  
I-Hua Chen ◽  
Jiann-Hsiung Wang ◽  
Shih-Jen Chou ◽  
Tsung-Hsien Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune System ◽  
Reference Genes ◽  
Animal Health ◽  
Rna Extraction ◽  
Disease Onset ◽  
Delphinapterus Leucas ◽  
Amplification Efficiency ◽  
Beluga Whales ◽  
Qrt Pcr

Cytokines are fundamental for a functioning immune system, and thus potentially serve as important indicators of animal health. Quantitation of mRNA using quantitative reverse transcription polymerase chain reaction (qRT-PCR) is an established immunological technique. It is particularly suitable for detecting the expression of proteins against which monoclonal antibodies are not available. In this study, we developed a probe-based quantitative gene expression assay for immunological assessment of captive beluga whales (Delphinapterus leucas) that is one of the most common cetacean species on display in aquariums worldwide. Six immunologically relevant genes (IL-2Rα, -4, -10, -12, TNFα, and IFNγ) were selected for analysis, and two validated housekeeping genes (PGK1 and RPL4) with stable expression were used as reference genes. Sixteen blood samples were obtained from four animals with different health conditions and stored in RNAlater™ solution. These samples were used for RNA extraction followed by qRT-PCR analysis. Analysis of gene transcripts was performed by relative quantitation using the comparative Cq method with the integration of amplification efficiency and two reference genes. The expression levels of each gene in the samples from clinically healthy animals were normally distributed. Transcript outliers for IL-2Rα, IL-4, IL-12, TNFα, and IFNγ were noticed in four samples collected from two clinically unhealthy animals. This assay has the potential to identify immune system deviation from normal state, which is caused by health problems. Furthermore, knowing the immune status of captive cetaceans could help both trainers and veterinarians in implementing preventive approaches prior to disease onset.

scholarly journals Hematology and Plasma Chemistry as Indicators of Health and Ecological Status In Beluga Whales, Delphinapterus Leucas

ARCTIC ◽  
2001 ◽  
Vol 54 (3) ◽  
Author(s):  
D.J. St. Aubin ◽  
S. Deguise ◽  
P.R. Richard ◽  
T.G. Smith ◽  
J.R. Geraci
Keyword(s):  
Ecological Status ◽  
Plasma Chemistry ◽  
Delphinapterus Leucas ◽  
Beluga Whales
Sign in / Sign up

Export Citation Format

Share Document