scholarly journals Inertial Focusing for Tumor Antigen-Dependent and -Independent Sorting of Rare Circulating Tumor Cells

2013 ◽  
Vol 5 (179) ◽  
pp. 179ra47-179ra47 ◽  
Author(s):  
E. Ozkumur ◽  
A. M. Shah ◽  
J. C. Ciciliano ◽  
B. L. Emmink ◽  
D. T. Miyamoto ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Tumor Antigen ◽  
Inertial Focusing

Inertial Focusing of Circulating Tumor Cells in Whole Blood at High Flow Rates using the Microfluidic CTCKey™ Device for CTC Enrichment

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Kaylee Judith Smith ◽  
Jessica Antoinette Jana ◽  
Anna Kaehr ◽  
Emma Purcell ◽  
Tyler Opdycke ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Treatment Decisions ◽  
Blood Stream ◽  
High Flow ◽  
Flow Rates ◽  
Inertial Focusing ◽  
Direct Treatment ◽  
Rare Cells

Circulating Tumor Cells (CTCs) are extremely rare cells shed from tumors into the blood stream. These cells can provide valuable information about their tumor of origin and direct treatment decisions...

Label-free Separation of Circulating Tumor Cells Using a Self-Amplified Inertial Focusing (SAIF) Microfluidic Chip

2020 ◽  
Vol 92 (24) ◽  
pp. 16170-16179
Author(s):  
Aynur Abdulla ◽  
Ting Zhang ◽  
Khan Zara Ahmad ◽  
Shanhe Li ◽  
Jiatao Lou ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Chip ◽  
Label Free ◽  
Inertial Focusing

scholarly journals Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

Lab on a Chip ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 1860-1876 ◽  
Author(s):  
Wujun Zhao ◽  
Yang Liu ◽  
Brittany D. Jenkins ◽  
Rui Cheng ◽  
Bryana N. Harris ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Cell Size ◽  
Circulating Tumor Cells ◽  
Tumor Antigen ◽  
Cell Separation ◽  
Size Variation ◽  
Cell Size Variation

Integrated ferrohydrodynamic cell separation (iFCS) explores cell magnetization in biocompatible ferrofluids and enriches CTCs in an antigen-independent and cell size variation-inclusive manner.

scholarly journals Enhanced Separation Efficiency and Purity of Circulating Tumor Cells Based on the Combined Effects of Double Sheath Fluids and Inertial Focusing

2021 ◽  
Vol 9 ◽  
Author(s):  
Bo-Wen Li ◽  
Kun Wei ◽  
Qi-Qi Liu ◽  
Xian-Ge Sun ◽  
Ning Su ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Purity ◽  
Separation Efficiency ◽  
Control Group ◽  
Combined Effects ◽  
Label Free ◽  
Inertial Focusing ◽  
Double Spiral ◽  
Spiral Microchannel

Circulating tumor cells (CTCs) play a crucial role in solid tumor metastasis, but obtaining high purity and viability CTCs is a challenging task due to their rarity. Although various works using spiral microchannels to isolate CTCs have been reported, the sorting purity of CTCs has not been significantly improved. Herein, we developed a novel double spiral microchannel for efficient separation and enrichment of intact and high-purity CTCs based on the combined effects of two-stage inertial focusing and particle deflection. Particle deflection relies on the second sheath to produce a deflection of the focused sample flow segment at the end of the first-stage microchannel, allowing larger particles to remain focused and entered the second-stage microchannel while smaller particles moved into the first waste channel. The deflection of the focused sample flow segment was visualized. Testing by a binary mixture of 10.4 and 16.5 μm fluorescent microspheres, it showed 16.5 μm with separation efficiency of 98% and purity of 90% under the second sheath flow rate of 700 μl min−1. In biological experiments, the average purity of spiked CTCs was 74% at a high throughput of 1.5 × 108 cells min−1, and the recovery was more than 91%. Compared to the control group, the viability of separated cells was 99%. Finally, we validated the performance of the double spiral microchannel using clinical cancer blood samples. CTCs with a concentration of 2–28 counts ml−1 were separated from all 12 patients’ peripheral blood. Thus, our device could be a robust and label-free liquid biopsy platform in inertial microfluidics for successful application in clinical trials.

High-Throughput Isolation of Circulating Tumor Cells Using Cascaded Inertial Focusing Microfluidic Channel

2018 ◽  
Vol 90 (7) ◽  
pp. 4397-4405 ◽  
Author(s):  
Aynur Abdulla ◽  
Wenjia Liu ◽  
Azarmidokht Gholamipour-Shirazi ◽  
Jiahui Sun ◽  
Xianting Ding
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Microfluidic Channel ◽  
Inertial Focusing

CTCtrap – Circulating Tumor Cells TheRapeutic APheresis: a novel biotechnology enabling personalized therapy for all cancer patients

2014 ◽  
Vol 74 (S 01) ◽  
Author(s):  
H Neubauer ◽  
N Kasprowicz ◽  
B Rack ◽  
C Vizler ◽  
M Scholz ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Personalized Therapy

The impact of HER2 phenotype of circulating tumor cells in metastatic breast cancer: a study in 107 patients

2014 ◽  
Vol 74 (S 01) ◽  
Author(s):  
M Wallwiener ◽  
AD Hartkopf ◽  
S Riethdorf ◽  
J Nees ◽  
FA Taran ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Metastatic Breast ◽  
The Impact
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