A new fractionation method for kappa- and lambda-carrageenans isolated from Gigartina pistillata (Rhodophyta)

2006 ◽  
Vol 122 ◽  
pp. 107-122
Author(s):  
Aziza Mouradi ◽  
Abdellah Amimi ◽  
Jean Mollet ◽  
Thierry Claude Givernaud
Keyword(s):  
Fractionation Method

Functional Relationship of Polymerization Sites of Vertebrate Fibrinogens

1979 ◽  
Author(s):  
C Cierniewski ◽  
T Krajewski ◽  
E Janiak
Keyword(s):  
Gel Electrophoresis ◽  
Functional Relationship ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Functional Similarity ◽  
Fractionation Method ◽  
Relationship Of ◽  
Fibrin Monomers ◽  
Fibrinogen Molecule ◽  
Cold Ethanol Fractionation

Various studies on the interaction of immobilized mammalian fibrinogen and fibrin monomers with some fibrinogen derivatives demonstrated the presence of two sets of polymerization sites in the mammalian fibrinogen molecule. We obtained the same results while investigating the fibrinogen molecules of other classes of vertebrates /Pisces. Amphibia. Aves/. Despite significant differences among their subunit structures, all of them contain polymerization sites homologous to mammalian counterparts. Moreover, due to great functional similarity, fibrinogen or fibrin monomers of the analyzed species of Pisces. Amphibia. Aves and Mammalia interacted in a specific way with immobilized pig fibrin monomers or fibrinogen, respectively. Using these pig affinity adsorbents, fibrinogen and fibrin monomers of different vertebrates were isolated directly from plasma and analyzed by SDS polyacrylamide gel electrophoresis. Polypeptide compositions of eluted proteins were identical to those obtained for corresponding fibrinogen preparations isolated by cold-ethanol fractionation method. It appears to indicate that the nature of polymerization sites in vertebrate fibrinogens is alike.

A rapid fractionation method for assessing key soil phosphorus parameters in agroecosystems

Geoderma ◽  
2021 ◽  
Vol 385 ◽  
pp. 114893
Author(s):  
Luciano C. Gatiboni ◽  
Leo M. Condron
Keyword(s):  
Soil Phosphorus ◽  
Fractionation Method

scholarly journals Acyl-CoA synthetase and the peroxisomal enzymes of β-oxidation in human liver. Quantitative analysis of their subcellular localization

1984 ◽  
Vol 224 (3) ◽  
pp. 709-720 ◽  
Author(s):  
M Bronfman ◽  
N C Inestrosa ◽  
F O Nervi ◽  
F Leighton
Keyword(s):  
Human Liver ◽  
Fatty Acyl ◽  
Single Step ◽  
Synthetase Activity ◽  
Beta Oxidation ◽  
Analysis Of Results ◽  
Fractionation Method ◽  
Hydroxyacyl Coa Dehydrogenase ◽  
Acyl Coa Oxidase ◽  
Rate Limiting

The presence of acyl-CoA synthetase (EC 6.2.1.3) in peroxisomes and the subcellular distribution of beta-oxidation enzymes in human liver were investigated by using a single-step fractionation method of whole liver homogenates in metrizamide continuous density gradients and a novel procedure of computer analysis of results. Peroxisomes were found to contain 16% of the liver palmitoyl-CoA synthetase activity, and 21% and 60% of the enzyme activity was localized in mitochondria and microsomal fractions respectively. Fatty acyl-CoA oxidase was localized exclusively in peroxisomes, confirming previous results. Human liver peroxisomes were found to contribute 13%, 17% and 11% of the liver activities of crotonase, beta-hydroxyacyl-CoA dehydrogenase and thiolase respectively. The absolute activities found in peroxisomes for the enzymes investigated suggest that in human liver fatty acyl-CoA oxidase is the rate-limiting enzyme of the peroxisomal beta-oxidation pathway, when palmitic acid is the substrate.

scholarly journals Subcellular analysis of starch metabolism in developing barley seeds using a non-aqueous fractionation method

2011 ◽  
Vol 63 (5) ◽  
pp. 2071-2087 ◽  
Author(s):  
Axel Tiessen ◽  
Annika Nerlich ◽  
Benjamin Faix ◽  
Christine Hümmer ◽  
Simon Fox ◽  
...  
Keyword(s):  
Starch Metabolism ◽  
Fractionation Method

IEF peptide fractionation method combined to shotgun proteomics enhances the exploration of rice milk proteome

2017 ◽  
Vol 537 ◽  
pp. 72-77 ◽  
Author(s):  
Marcello Manfredi ◽  
Jessica Brandi ◽  
Eleonora Conte ◽  
Paolo Pidutti ◽  
Fabio Gosetti ◽  
...  
Keyword(s):  
Shotgun Proteomics ◽  
Peptide Fractionation ◽  
Fractionation Method

Assessment of mercury mobility and bioavailability by fractionation method in sediments from coastal zone inundated by the 26 December 2004 tsunami in Thailand

2006 ◽  
Vol 51 (4) ◽  
pp. 527-536 ◽  
Author(s):  
Leonard Boszke ◽  
Artur Kowalski ◽  
Witold Szczuciński ◽  
Grzegorz Rachlewicz ◽  
Stanisław Lorenc ◽  
...  
Keyword(s):  
Coastal Zone ◽  
Fractionation Method ◽  
2004 Tsunami

scholarly journals Fractionation of Regenerated Silk Fibroin and Characterization of the Fractions

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6317
Author(s):  
Masaaki Aoki ◽  
Yu Masuda ◽  
Kota Ishikawa ◽  
Yasushi Tamada
Keyword(s):  
Mechanical Properties ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Gel Permeation Chromatography ◽  
Precipitation Process ◽  
Regenerated Silk Fibroin ◽  
Sds Page ◽  
Fractionation Method ◽  
Nonwoven Mats

The molecular weight (MW) of regenerated silk fibroin (RSF) decreases during degumming and dissolving processes. Although MW and the MW distribution generally affect polymer material processability and properties, few reports have described studies examining the influences of MW and the distribution on silk fibroin (SF) material. To prepare different MW SF fractions, the appropriate conditions for fractionation of RSF by ammonium sulfate (AS) precipitation process were investigated. The MW and the distribution of each fraction were found using gel permeation chromatography (GPC) and SDS-polyacrylamide electrophoresis (SDS-PAGE). After films of the fractionated SFs formed, the secondary structure, surface properties, and cell proliferation of films were evaluated. Nanofiber nonwoven mats and 3D porous sponges were fabricated using the fractionated SF aqueous solution. Then, their structures and mechanical properties were analyzed. The results showed AS precipitation using a dialysis membrane at low temperature to be a suitable fractionation method for RSF. Moreover, MW affects the nanofiber and sponge morphology and mechanical properties, although no influence of MW was observed on the secondary structure or crystallinity of the fabricated materials.

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