In VitroandIn VivoSynergy of the Oxadiazole Class of Antibacterials with β-Lactams
ABSTRACTThe oxadiazole antibacterials target the bacterial cell wall and are bactericidal. We investigated the synergism of ND-421 with the commonly used β-lactams and non-β-lactam antibiotics by the checkerboard method and by time-kill assays. ND-421 synergizes well with β-lactam antibiotics, and it also exhibits a long postantibiotic effect (4.7 h). We also evaluated thein vivoefficacy of ND-421 in a murine neutropenic thigh infection model alone and in combination with oxacillin. ND-421 hasin vivoefficacy by itself in a clinically relevant infection model (1.49 log10bacterial reduction for ND-321 versus 0.36 log10for linezolid with NRS119) and acts synergistically with β-lactam antibioticsin vitroandin vivo, and the combination of ND-421 with oxacillin is efficacious in a mouse neutropenic thigh methicillin-resistantStaphylococcus aureus(MRSA) infection model (1.60 log10bacterial reduction). The activity of oxacillin was potentiated in the presence of ND-421, as the strain would have been resistant to oxacillin otherwise.