Development of an Allele-Specific PCR for Detection of the K65R Resistance Mutation in Patients Infected with Subtype C Human Immunodeficiency Virus Type 1
2009 ◽
Vol 54
(2)
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pp. 907-911
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Keyword(s):
ABSTRACTThe selection of drug-resistant variants of human immunodeficiency virus type 1 (HIV-1) is an impediment to the efficiency of antiretroviral (ARV) therapy. We have developed an allele-specific real-time PCR assay to explore the presence of K65R minority species among treated HIV-1 subtype B and C infections. Thirty HIV-1 subtype C- and 26 subtype B-infected patients lacking K65R as determined by conventional sequencing methods were studied, and viral minority species were found in four HIV-1 subtype C samples.
2000 ◽
Vol 74
(23)
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pp. 11286-11295
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2003 ◽
Vol 77
(6)
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pp. 3832-3837
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Keyword(s):
1999 ◽
Vol 73
(5)
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pp. 4427-4432
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Keyword(s):
2005 ◽
Vol 21
(5)
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pp. 379-394
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1999 ◽
Vol 73
(1)
◽
pp. 152-160
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Keyword(s):
2004 ◽
Vol 78
(6)
◽
pp. 2841-2852
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2006 ◽
Vol 80
(10)
◽
pp. 4698-4704
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2003 ◽
Vol 77
(2)
◽
pp. 1021-1038
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Keyword(s):