scholarly journals Nucleotide Sequence Analysis of Integrative Conjugative Element Tn5253of Streptococcus pneumoniae

2013 ◽  
Vol 58 (2) ◽  
pp. 1235-1239 ◽  
Author(s):  
Francesco Iannelli ◽  
Francesco Santoro ◽  
Marco R. Oggioni ◽  
Gianni Pozzi
Keyword(s):  
Streptococcus Pneumoniae ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Streptococcus Pyogenes ◽  
Open Reading Frames ◽  
Nucleotide Sequence Analysis ◽  
Conjugative Transposon ◽  
Content Type ◽  
Integrative Conjugative Element ◽  
Reading Frames

ABSTRACTConjugative transposon Tn5253, an integrative conjugative element (ICE) ofStreptococcus pneumoniaecarrying thecatandtet(M) genes, was shown to be 64,528 bp in size and to contain 79 open reading frames, of which only 38 could be annotated. Two distinct genetic elements were found integrated into Tn5253: Tn5251(18,033 bp), of the Tn916-Tn1545family of ICEs, and Ωcat(pC194) (7,627 bp), which could not conjugate but was capable of intracellular mobility by excision, circularization, and integration by homologous recombination. The highest conjugation frequency of Tn5253was observed whenStreptococcus pyogeneswas the donor (6.7 × 10−3transconjugants/donor).

scholarly journals Identification of three new Ig lambda-like genes in man.

1986 ◽  
Vol 163 (2) ◽  
pp. 425-435 ◽  
Author(s):  
H Chang ◽  
E Dmitrovsky ◽  
P A Hieter ◽  
K Mitchell ◽  
P Leder ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Gene Family ◽  
Southern Blot ◽  
Restriction Enzyme ◽  
Open Reading Frames ◽  
Nucleotide Sequence Analysis ◽  
Evolutionary Lineage ◽  
Functional Locus ◽  
Reading Frames

Three new human lambda L chain-like Ig genes are identified by restriction enzyme and nucleotide sequence analysis. Two genes, 14.1 and 16.1, have intact J and C regions, and are potentially functional, with open reading frames. A third gene, 18.1, is a pseudogene. The evolutionary lineage of these genes compared to the known functional locus lambda C1-lambda C6 can be surmised from Southern blot and nucleotide homologies. This study demonstrates that the human lambda gene family is more complex than previously recognized.

scholarly journals Selection of Virulence-Associated Determinants ofStreptococcus suis Serotype 2 by In Vivo Complementation

2001 ◽  
Vol 69 (3) ◽  
pp. 1961-1966 ◽  
Author(s):  
Hilde E. Smith ◽  
Herma Buijs ◽  
Henk J. Wisselink ◽  
Norbert Stockhofe-Zurwieden ◽  
Mari A. Smits
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Genomic Library ◽  
Open Reading Frames ◽  
Pathogenic Strain ◽  
Nucleotide Sequence Analysis ◽  
Suis Serotype ◽  
Reading Frames ◽  
Selection Of

ABSTRACT Within Streptococcus suis serotype 2, pathogenic, weakly pathogenic, and nonpathogenic strains can be found. We introduced a genomic library of a pathogenic strain into a weakly pathogenic strain. After infection of the library into young piglets pathogenic transformants were selected. One specific transformant containing a 3-kb fragment of the pathogenic strain appeared to be dominantly enriched in diseased pigs. The observed enrichment was not tissue specific. The selected fragment, when introduced into two different weakly pathogenic strains, increased the virulence of these strains considerably. In contrast, introduction of the corresponding fragment of a weakly pathogenic strain had only minor effects on virulence. Nucleotide sequence analysis of the selected fragment of the pathogenic strain revealed the presence of two potential open reading frames, both of which were found to be mutated in the corresponding fragment of the weakly pathogenic strain. These data strongly suggest that the selected fragment contains determinants important for virulence.

scholarly journals Complete Genome Sequence of Salmonella Bacteriophage SS3e

2012 ◽  
Vol 86 (18) ◽  
pp. 10253-10254 ◽  
Author(s):  
Sung-Hun Kim ◽  
Jeong-Hyun Park ◽  
Bok-Kwon Lee ◽  
Hyuk-Joon Kwon ◽  
Ji-Hyun Shin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Genomic Sequence ◽  
Enterobacter Cloacae ◽  
Open Reading Frames ◽  
Lytic Bacteriophage ◽  
Content Type ◽  
Double Stranded Dna ◽  
Genomic Sequence Analysis ◽  
Reading Frames

ASalmonellalytic bacteriophage, SS3e, was isolated, and its genome was sequenced completely. This phage is able to lyse not only variousSalmonellaserovars but alsoEscherichia coli,Shigella sonnei,Enterobacter cloacae, andSerratia marcescens, indicating a broad host specificity. Genomic sequence analysis of SS3e revealed a linear double-stranded DNA sequence of 40,793 bp harboring 58 open reading frames, which is highly similar toSalmonellaphages SETP13 and MB78.

Nucleotide sequence analysis of small cryptic plasmid pGP2 from Acetobacter estunensis

Biologia ◽  
2011 ◽  
Vol 66 (2) ◽  
Author(s):  
Peter Grones ◽  
Jozef Grones
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Shuttle Vector ◽  
Restriction Enzymes ◽  
Open Reading Frames ◽  
Nucleotide Sequence Analysis ◽  
Α Subunit ◽  
Rep Protein ◽  
Class B ◽  
Derivative Plasmid

AbstractComplete nucleotide sequence of plasmid pGP2 from Acetobacter estunensis GP2 was identified after initial cloning of EcoRI fragment followed by preparation of deletion derivatives. Its size was defined to 2,797 bp and several sites for several restriction enzymes were revealed by DNA sequencing. Sequence analysis predicts three putative open reading frames (ORFs). ORF1 shows significant identity with the bacterial excinuclease α-subunit, ORF2 is a putative replication protein with low similarity with other Acetobacter plasmid’s replication proteins, and ORF3 encodes a class B acid phosphatase/phosphotransferase. The replication module comprises a DnaA box like sequence, direct repeats, a potential prokaryotic promoter and a rep gene. The rep module is similar with several θ-replicating, iteron-containing modules from plasmids, suggesting pGP2 replication may follow the same course. Any phenotypic character determinant gene is absent in pGP2, suggesting this plasmid to be cryptic. However, a pGP2 derivative plasmid, containing the putative pGP2 rep region, can replicate and is stably maintained in Acetobacter and Escherichia coli strains; it can also carry foreign DNA fragments. Thus, pGP2-X could serve as a cloning shuttle vector between these bacteria. Prepared deletion derivatives of plasmid pGP2 suggested that Rep protein is essential for plasmid replication in host bacteria. In its natural host, A. estunensis GP2, pGP2 maintains a four-times lower copy number than in E. coli.

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