Automated Thermal Cycling Is Superior to Traditional Methods for Nucleotide Sequencing ofblaSHV Genes
1999 ◽
Vol 43
(12)
◽
pp. 2960-2963
◽
Keyword(s):
ABSTRACT Genes encoding SHV-1 and SHV-2 were sequenced by different methods. Nucleotide sequencing of the coding strand by standard dideoxy-chain termination methods resulted in errors in the interpretation of the nucleotide sequence and the derived amino acid sequence in two main regions which corresponded to nucleotide and amino acid changes that had been reported previously. The automated thermal cycling method was clearly superior and consistently resulted in the correct sequences for these genes.
2001 ◽
Vol 45
(4)
◽
pp. 1278-1280
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Keyword(s):
1988 ◽
Vol 62
(4)
◽
pp. 1186-1193
◽
Amino acid sequence of the gene 0.3 protein of bacteriophage T7 and nucleotide sequence of its mRNA.
1981 ◽
Vol 256
(5)
◽
pp. 2579-2585
1984 ◽
Vol 259
(7)
◽
pp. 4320-4326
◽
1990 ◽
Vol 189
(1)
◽
pp. 73-81
◽
1987 ◽
Vol 262
(17)
◽
pp. 8131-8137
◽
1998 ◽
Vol 96
(1-2)
◽
pp. 93-110
◽
Keyword(s):