scholarly journals Updated Sequence Information and Proposed Nomenclature for blaTEM Genes and Their Promoters

2000 ◽  
Vol 44 (11) ◽  
pp. 3232-3234 ◽  
Author(s):  
V. Leflon-Guibout ◽  
B. Heym ◽  
M.-H. Nicolas-Chanoine

ABSTRACT The nucleotide sequences of 59 bla TEM genes encoding inhibitor-resistant TEM enzymes showed great genetic variability and were associated with different types of promoters. These findings led us to suggest an updatedbla TEM gene nomenclature based on the origin of the bla TEM gene (bla TEM-1A, bla TEM-1B,bla TEM-1C, bla TEM-1D,bla TEM-1E, andbla TEM-1F) and the promoter type.

2018 ◽  
Vol 44 ◽  
pp. S77-S78
Author(s):  
D.H. Ryu ◽  
S.W. Lee ◽  
S.J. Lee ◽  
H. Jeong ◽  
C.H. Lee ◽  
...  

2018 ◽  
Vol 73 (9-10) ◽  
pp. 375-389 ◽  
Author(s):  
Libao Cheng ◽  
Huiying Liu ◽  
Runzhi Jiang ◽  
Shuyan Li

AbstractThe formation of adventitious roots (ARs) is an important process for lotus (Nelumbo nucifera), which does not have a well-formed main root. In lotus, the removal of leaves above the waterline significantly promoted AR formation, while the removal of leaves below the waterline inhibited AR formation. Proteins were identified using isobaric tags for relative and absolute quantization technique. The number of proteins decreased with increasing sequencing coverage, and most of the identified proteins had fewer than 10 peptides. In the A1/A0 and A2/A1 stages, 661 and 154 proteins showed increased abundance, respectively, and 498 and 111 proteins showed decreased abundance, respectively. In the B1/B0 and B2/B1 stages, 498 and 436 proteins showed increased abundance, respectively, and 358 and 348 proteins showed decreased abundance, respectively. Among the proteins showing large differences in abundance, 17 were identified as being related to AR formation. Proteins involved in the glycolytic pathway and the citrate cycle showed differences in abundance between the two types of leaf removal. The transcriptional levels of nine genes encoding relevant proteins were assessed by quantitative polymerase chain reaction. The results of this study illustrate the changes in metabolism after different types of leaf removal during AR formation in lotus.


1999 ◽  
Vol 87 (6) ◽  
pp. 721-731 ◽  
Author(s):  
Noboru Takizawa ◽  
Toshiya Iida ◽  
Takashi Sawada ◽  
Kazuhiro Yamauchi ◽  
Yue-Wu Wang ◽  
...  

Blood ◽  
1992 ◽  
Vol 80 (8) ◽  
pp. 2066-2073 ◽  
Author(s):  
M Lubbert ◽  
W Oster ◽  
WD Ludwig ◽  
A Ganser ◽  
R Mertelsmann ◽  
...  

Abstract Expression of numerous genes encoding myeloid-specific proteins is tightly regulated during normal myeloid differentiation. This heterogeneous group of genes thus offers a tool for dissection of different maturational steps of myelopoiesis. We have previously shown that the human myeloperoxidase (MPO) gene is modified at numerous CpG residues in normal myeloid cells and in myeloid cell lines in a development-specific and expression-associated manner. In the present work, we have applied this type of methylation analysis to primary leukemia myeloid cell samples. We found that expression of MPO messenger RNA (mRNA) is grossly limited to leukemias of late myeloblastic and promyelocytic stages; expression is thus associated with progressive demethylation in the 5′ region of the MPO gene. This modification was not global. Low-level induction of MPO mRNA expression in very immature leukemic cells using phorbol ester was not accompanied by progressive demethylation. This type of methylation analysis of a myeloid-specific gene may yield a molecular indicator for different types of myeloid leukemias.


1971 ◽  
Vol 49 (9) ◽  
pp. 1559-1563
Author(s):  
Dianne Fahselt

A survey was made of the flavonoid components in Dicentra canadensis to investigate its relationship with other species in the genus. This study supports the view that D. canadensis is most closely allied to D. cucullaria. Flavonoids suggest an even closer alliance between the two than does morphology, while alkaloid data reported in the literature would suggest a more distant one. Thus, the degree of relationship supported by morphological evidence is intermediate to what is supported by two different types of biochemical evidence. A large proportion of the total flavonoids in D. canadensis is detected with considerably less than 100% frequency within the species. This variability may reflect some genetic variability between populations, and is almost certainly due in part to the difficulty in detection of weaker spots.


1990 ◽  
Vol 18 (15) ◽  
pp. 4589-4589 ◽  
Author(s):  
E. Jamet ◽  
C. Fargeas ◽  
A. Durr ◽  
J. Fleck

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