Flavonoid components of Dicentra canadensis (Fumariaceae)

1971 ◽  
Vol 49 (9) ◽  
pp. 1559-1563
Author(s):  
Dianne Fahselt

A survey was made of the flavonoid components in Dicentra canadensis to investigate its relationship with other species in the genus. This study supports the view that D. canadensis is most closely allied to D. cucullaria. Flavonoids suggest an even closer alliance between the two than does morphology, while alkaloid data reported in the literature would suggest a more distant one. Thus, the degree of relationship supported by morphological evidence is intermediate to what is supported by two different types of biochemical evidence. A large proportion of the total flavonoids in D. canadensis is detected with considerably less than 100% frequency within the species. This variability may reflect some genetic variability between populations, and is almost certainly due in part to the difficulty in detection of weaker spots.

2000 ◽  
Vol 44 (11) ◽  
pp. 3232-3234 ◽  
Author(s):  
V. Leflon-Guibout ◽  
B. Heym ◽  
M.-H. Nicolas-Chanoine

ABSTRACT The nucleotide sequences of 59 bla TEM genes encoding inhibitor-resistant TEM enzymes showed great genetic variability and were associated with different types of promoters. These findings led us to suggest an updatedbla TEM gene nomenclature based on the origin of the bla TEM gene (bla TEM-1A, bla TEM-1B,bla TEM-1C, bla TEM-1D,bla TEM-1E, andbla TEM-1F) and the promoter type.


2011 ◽  
Vol 11 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Cicero Almeida ◽  
Edson Perito Amorim ◽  
José Fernandes Barbosa Neto ◽  
Julio Alves Cardoso Filho ◽  
Maria Jane Cruz de Melo Sereno

The maize (Zea mays L. ssp. mays) has several related species, called teosinte, which are distributed in various subspecies of Zea and other genera. Among the different types of corn, sweet corn shows a great potential for human food. This type was originated from mutations, which increased the amount of polysaccharide in the endosperm. In Brazil there are populations of sweet corn, common maize and teosinte, however, little is known about their genetic variability. Hence, the aim of this present paper was to analyze the genetic variability in two populations of sweet corn (BR 400 and BR 402), two common corn (Pampa and Suwan) and teosinte, using microsatellite markers. The results showed a low intra-population genetic variability in populations of maize, and high variability for the population of teosinte, suggesting that the maize populations may have limitations in future cycles of breeding.


2020 ◽  
Vol 14 (1) ◽  
pp. 16
Author(s):  
RULLY DYAH PURWATI ◽  
SUDJINDRO SUDJINDRO ◽  
ENDANG KARTINI ◽  
SUDARSONO SUDARSONO

ABSTRAK<br />Mutasi in vitro dengan perlakuan mutagen dapat digunakan untuk<br />meningkatkan keragaman genetika abaka melalui keragaman somaklonal.<br />Penelitian yang dilakukan bertujuan untuk: (1) menentukan konsentrasi<br />optimum EMS untuk induksi keragaman somaklonal dalam kultur kalus<br />embriogen abaka, (2) meregenerasikan bibit abaka varian dari kalus<br />embriogen yang diberi perlakuan EMS, dan (3) mengevaluasi tipe dan<br />frekuensi keragaman karakter kualitatif dan kuantitatif di antara populasi<br />tanaman mutan abaka yang diperoleh dari regenerasi kalus embriogen<br />yang diberi perlakuan EMS. Penelitian dilakukan mulai bulan Agustus<br />2003 sampai Agustus 2006 di Laboratorium Kultur Jaringan dan Kebun<br />Percobaan Karangploso, Malang pada Balai Penelitian Tanaman<br />Tembakau dan Serat, Malang (Balittas). Hasil penelitian menunjukkan<br />bahwa 0,6% EMS merupakan konsentrasi optimum karena pada<br />konsentrasi tersebut diperoleh keragaman somaklonal paling banyak.<br />Varian yang diperoleh menunjukkan karakter kualitatif dan kuantitatif<br />abnormal. Tipe varian tersebut umumnya bersifat negatif dan kurang<br />menguntungkan dibandingkan dengan populasi standar. Tipe dan frekuensi<br />keragaman kualitatif dan kuantitatif pada klon Tangongon berbeda dengan<br />klon Sangihe-1, mengindikasikan adanya pengaruh genotipe terhadap<br />keragaman somaklonal. Varian dari abaka klon Tangongon dengan<br />produksi serat tertinggi (161,0 g dan 154,0 g/tanaman) diperoleh dari<br />perlakuan EMS 0,3% (T1 28.1.1 dan T1 11.2.2), sedangkan dari klon<br />Sangihe-1, hasil serat tertinggi (35,0 g dan 40,0 g/tanaman) diperoleh dari<br />perlakuan EMS 0,6% (S4 28.1.0 dan S4 56.2.0). Produktivitas tersebut<br />lebih rendah dibandingkan dengan produksi serat tanaman kontrol klon<br />Tangongon (193,0 g/tanaman) dan Sangihe-1 (70 g/tanaman).<br />Kata kunci : Abaka, Musa textilis, keragaman somaklonal, EMS, muta-<br />genesis, in vitro, hasil, Jawa Timur<br />ABSTRACT<br />Genetic Variability of Abaca Variants Induced by<br />Ethylmethane Sulphonate (EMS)<br />In vitro mutation with mutagen treatment can be used to increase<br />the genetic variability of abaca by inducing somaclonal variation. The<br />objectives of the experiments were to (1) determine optimum concen-<br />tration of EMS to induce abaca somaclonal variation, (2) produce abaca<br />lines from EMS treated embryogenic calli and evaluate their performance<br />in the field, and (3) evaluate type and frequency of qualitative and<br />quantitative variant characters among regenerated abaca lines. This<br />experiment was conducted in Tissue Culture Laboratory and Karangploso<br />Experiment Station of Indonesian Tobacco and Fibre Crops Research<br />Institute (ItoFCRI) Malang from August 2003 to August 2006. The results<br />showed that EMS treatment on abaca embryogenic calli induced variation,<br />and the optimum EMS concentration was 0.6%. The variants exhibited a<br />number of abnormal qualitative and quantitative characters which were<br />generally negative characters since they showed lower value as compared<br />to control population. The presence of different types of qualitative and<br />quantitative variant characters was genotype dependent. Variants from<br />abaca clone Tangongon having the highest fibre yield (161.0 g and 154.0<br />g/plant) were obtained from 0.3% EMS treatment (T1 28.1.1 and T1 11.2.2<br />variants). While variants from abaca clone Sangihe-1 having the highest<br />fibre yield (35.0 g and 40.0 g/plant) were obtained from 0.6% EMS<br />treatment (S4 28.1.0 and S4 56.2.0 variants). The fibre yield of control<br />clones, Tangongon and Sangihe-1, were 193.0 g and 70 g/plant,<br />respectively.<br />Key words: Abaca, Musa textilis, induced mutation, somaclonal<br />variation, field evaluation, yield, East Java


2021 ◽  
Vol 233 ◽  
pp. 02033
Author(s):  
Feng Xuehua ◽  
Song Zurong ◽  
Wang Jun ◽  
Fang Jie

The paper determined the content of total flavonoids in different types and parts of rhizome shoot and winter bamboo shoots by UV spectrophotometry for comparison. The results showed that the bamboo shoot sheath had a higher level of total flavonoids than in the bamboo shoots, and the rhizome shoot had a higher level of total flavonoids than the winter bamboo shoots in different parts.


2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Joana Carvalho-Pereira ◽  
Catarina Vaz ◽  
Catarina Carneiro ◽  
Célia Pais ◽  
Paula Sampaio

The secreted aspartic proteases (Saps) are among the most studied virulence determinants inCandida albicans. These proteins are translated as pre-pro-enzymes consisting of a signal sequence followed by a propeptide and the mature enzyme. The propeptides of secreted proteinases are important for the correct processing, folding/secretion of the mature enzyme. In this study, the DNA sequences ofC. albicansSaps were screened and a microsatellite was identified inSAP8propeptide region. The genetic variability of the repetitive region of Sap8 propeptide was determined in 108C. albicansindependent strains isolated from different types of infection: oral infection (OI), oral commensal (OC), vulvovaginal candidiasis (VVC), and bloodstream infections (BSI). Nine different propeptides for Sap8 processing were identified whose frequencies varied with the type of infection. OC strains presented the highest gene diversity while OI isolated the lowest. The contribution of the Saps to mucosal and systemic infections has been demonstrated and recently Sap8 has been implicated in the cleavage of a signalling glycoprotein that leads to Cek1-MAPK pathway activation. This work is the first to identify a variable microsatellite in the propeptide of a secreted aspartic protease and brings new insights into the variability of Sap8.


2021 ◽  
Vol 9 (1) ◽  
pp. 95
Author(s):  
Maria Elfira Semana ◽  
Pande Ketut Diah Kencana ◽  
I Gusti Ngurah Apriadi Aviantara

ABSTRAK Bambu tabah termasuk kingdom Plantae, divisi Magnoliophyta, kelas Monocotyledoneae, ordo Graminales, famili Gramineae, sub famili Bambusoideae genus Gigantochloa, spesies Gigantochloa nigrociliata Buse-Kurz. Bambu tabah merupakan varietas asli yang berasal dari Pupuan-Tabanan dan banyak dibudidayakan di desa Payangan Gianyar. Penelitian ini dilakukan degan tujuan untuk menentukan kemasan yang dapat mempertahankan karakteristik yang terdapat pada teh daun bambu tabah dengan perlakuan pengemasan menggunakan tiga jenis kemasan yang berbeda yang disimpan pada inkubator dengan suhu 30 °C ± 3 dengan lama penyimpanan enam minggu. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari dua faktor yaitu penggunaan jenis kemasan yang berbeda dan lama waktu penyimpanan. Faktor pertama terdiri dari tiga taraf yaitu, (K1) : Alumunium Foil, (K2) : Plastik Polipropilen (PP), dan (K3) : Paper Sack. Faktor kedua terdiri dari tiga taraf yaitu, (T1) : 14 hari penyimpanan, (T2) : 28 hari penyimpanan, dan (T3) : 42 hari penyimpanan. Parameter yang diamati dalam penelitian terdiri dari Kadar Air, Laju Pengeringan, pH, Total Asam, Total Fenol, Total Flavonoid, dan Uji Organoleptik terdiri dari warna, aroma, dan rasa pada daun teh bambu tabah yang diseduh. Hasil penelitian menunjukkan bahwa kandungan fenol tertinggi terdapat pada perlakuan penggunaan jenis kemasan alumunium foil selama penyimpanan 42 hari yaitu 119.354 mg/100g dan total flavonoid yang tertinggi atau terbaik terdapat pada perlakuan penggunaan jenis kemasan alumunium foil selama penyimpanan 42 hari yaitu 27.419 mg/100g.  ABSTRACT Rigid bamboos include kingdom Plantae, division Magnoliophyta, class Monocotyledoneae, order Graminales, family Gramineae, sub family Bambusoideae genus Gigantochloa, species Gigantochloa nigrociliata Buse-Kurz Tabah bamboo is a native variety originating from Pupuan-Tabanan and is widely cultivated in Payangan Gianyar village. This research was conducted with the aim of determining the packaging that can maintain the characteristics contained in tabah bamboo leaf tea with packaging treatment using three different types of packaging stored in an incubator at a temperature of 30 ° C ± 3 with a storage time of six weeks. This study used a completely randomized design (CRD) which consisted of two factors, namely the use of different types of packaging and storage time. The first factor consists of three levels, namely, (K1): Aluminum Foil, (K2): Polypropylene Plastic (PP), and (K3): Paper Sack. The second factor consists of three levels, namely, (T1): 14 days of storage, (T2): 28 days of storage, and (T3): 42 days of storage. The parameters observed in the study consisted of moisture content, drying rate, pH, total acid, total phenol, total flavonoids, and organoleptic tests consisting of color, aroma, and taste of brewed tabah bamboo tea leaves. The results showed that the highest phenol content was found in the treatment of using aluminum foil packaging for 42 days, which was 119,354 mg / 100g and the highest or best total flavonoids were found in the treatment of using aluminum foil packaging for 42 days, namely 27,419 mg / 100g.  


Chemija ◽  
2019 ◽  
Vol 30 (3) ◽  
Author(s):  
Vaida Vaškelienė ◽  
Rasa Šlinkšienė

For the extraction, rowanberries (collected in 2017 and 2018) and aqueous solutions of various concentrations of ethanol (50%, 60%, 70%, 80% and 96%) in a ratio of 1:10 were used. Different types of extraction methods (extraction under static conditions, Soxhlet extraction and ultrasound assisted extraction) were tested. Using different types of extraction methods, it was found that irrespective of the method chosen when the concentration of ethanol increases, the concentration of total flavonoids also increases. Using collected rowanberries in 2017, the highest and similar numerical value of the concentration of total flavonoids was reached when an ultrasound bath was used 20 min for extraction (0.512%) using a magnetic stirrer for 90 min (0.498%). In both cases, a 96% aqueous ethanol solution was used. The highest total concentration of flavonoids in the extracts of rowanberries collected in 2018, obtained under static extraction conditions, using a 96% aqueous ethanol solution, was 0.525%, in 2017 it was 0.418%.


Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 1147-1152 ◽  
Author(s):  
Sergey Gavrilets

Abstract I formulate and study a series of simple one-locus two-allele models for maternal (parental) selection. I show that maternal (parental) selection can result in simultaneous stability of equilibria of different types. Thus, in the presence of maternal (parental) selection the outcome of population evolution can significantly depend on initial conditions. With maternal selection, genetic variability can be maintained in the population even if none of the offspring of heterozygous mothers survive. I demonstrate that interactions of maternal and paternal selection can result in stable oscillations of genotype frequencies. A necessary condition for cycling is strong selection.


1986 ◽  
Vol 23 (04) ◽  
pp. 851-858 ◽  
Author(s):  
P. J. Brockwell

The Laplace transform of the extinction time is determined for a general birth and death process with arbitrary catastrophe rate and catastrophe size distribution. It is assumed only that the birth rates satisfyλ0= 0,λj&gt; 0 for eachj&gt; 0, and. Necessary and sufficient conditions for certain extinction of the population are derived. The results are applied to the linear birth and death process (λj=jλ, µj=jμ) with catastrophes of several different types.


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