scholarly journals Development and Preliminary Evaluation of a Loop-Mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples

2007 ◽  
Vol 73 (17) ◽  
pp. 5660-5662 ◽  
Author(s):  
Panagiotis Karanis ◽  
Oriel Thekisoe ◽  
Klytaimnistra Kiouptsi ◽  
Jerry Ongerth ◽  
Ikuo Igarashi ◽  
...  
Keyword(s):  
Diagnostic Tool ◽  
Water Samples ◽  
Epidemiologic Studies ◽  
Isothermal Amplification ◽  
Sensitive Detection ◽  
Preliminary Evaluation ◽  
Fecal Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Cryptosporidium Oocysts ◽  
Amplification Procedure

ABSTRACT A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid and high-sensitive detection of Cryptosporidium in water samples

2009 ◽  
Vol 60 (8) ◽  
pp. 2167-2172 ◽  
Author(s):  
A. Inomata ◽  
N. Kishida ◽  
T. Momoda ◽  
M. Akiba ◽  
S. Izumiyama ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Water Samples ◽  
Cryptosporidium Oocyst ◽  
Lamp Assay ◽  
Test Tube ◽  
Isothermal Amplification ◽  
Sensitive Detection ◽  
Loop Mediated Isothermal Amplification ◽  
Cryptosporidium Oocysts ◽  
Amplification Assay

We describe a novel assay for simple, rapid and high-sensitive detection of Cryptosporidium oocysts in water samples using a reverse transcription-loop-mediated isothermal amplification (RT-LAMP). The assay is based on the detection of 18S rRNA specific for Cryptosporidium oocysts. The detection limit of the developed RT-LAMP assay was as low as 6 × 10−3 oocysts/test tube, which theoretically enables us to detect a Cryptosporidium oocyst and perform duplicated tests even if water samples contain only one oocyst. The developed RT-LAMP assay could more sensitively detect Cryptosporidium oocysts in real water samples than the conventional assay based on microscopic observation.

scholarly journals Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification

2015 ◽  
Vol 65 (1) ◽  
pp. 20-29 ◽  
Author(s):  
PARK Byung-Yong ◽  
SHIM Kwan-Seob ◽  
KIM Won-Il ◽  
HOSSAIN Md Mukter ◽  
KIM Bumseok ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Conventional Pcr ◽  
Fecal Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Time Loop

Abstract A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100 L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples.

scholarly journals Rapid and sensitive detection of Nosema bombycis using loop-mediated isothermal amplification and colorimetric nanogold

ScienceAsia ◽  
2019 ◽  
Vol 45 (3) ◽  
pp. 268
Author(s):  
Weijiang Dai ◽  
Jingru Qi ◽  
Hongli Chen ◽  
Zhilin Zhang ◽  
Ruisha Shang ◽  
...  
Keyword(s):  
Isothermal Amplification ◽  
Sensitive Detection ◽  
Nosema Bombycis ◽  
Loop Mediated Isothermal Amplification
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