scholarly journals Construction of a Library of Human Glycosyltransferases Immobilized in the Cell Wall of Saccharomyces cerevisiae

2006 ◽  
Vol 72 (11) ◽  
pp. 7003-7012 ◽  
Author(s):  
Yoh-ichi Shimma ◽  
Fumie Saito ◽  
Fumi Oosawa ◽  
Yoshifumi Jigami
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Yeast Cell ◽  
Protein Disulfide Isomerase ◽  
Immobilized Enzymes ◽  
Enzymatic Activities ◽  
Yeast Cell Wall ◽  
Yeast Protein ◽  
A Cell ◽  
Protein Disulfide

ABSTRACT Fifty-one human glycosyltransferases were expressed in Saccharomyces cerevisiae as immobilized enzymes and were assayed for enzymatic activities. The stem and catalytic regions of sialyl-, fucosyl-, galactosyl-, N-acetylgalactosaminyl-, and N-acetylglucosaminyltransferases were fused with yeast cell wall Pir proteins, which anchor glycosyltransferases at the yeast cell wall glucan. More than 75% of expressed recombinant glycosyltransferases retained their enzymatic activities in the yeast cell wall fraction and will be used as a human glycosyltransferase library. In increasing the enzymatic activities of immobilized glycosyltransferases, several approaches were found to be effective. Additional expression of yeast protein disulfide isomerase increased the expression levels and activities of polypeptide N-acetylgalactosaminyltransferases and other glycosyltransferases. PIR3 and/or PIR4 was more effective than PIR1 as a cell wall anchor when the Pir-glycosyltransferase fusions were expressed under the control of the constitutive glyceraldehyde-3-phosphate dehydrogenase promoter. Oligosaccharides such as Lewis x, Lewis y, and H antigen were successfully synthesized using this immobilized glycosyltransferase library, indicating that the Pir-fused glycosyltransferases are useful for the production of various human oligosaccharides.

scholarly journals Broiler chick performance using Saccharomyces cerevisiae yeast cell wall as an anti-mycotoxin additive

2021 ◽  
Vol 66 (No. 2) ◽  
pp. 65-72
Author(s):  
Vinícius Machado dos Santos ◽  
Gabriel da Silva Oliveira ◽  
Cristina Amorim Ribeiro de Lima ◽  
Fernando Augusto Curvello
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Yeast Cell ◽  
Broiler Chicken ◽  
Live Weight ◽  
Yeast Cell Wall ◽  
Performance Parameters ◽  
Feed Conversion ◽  
Performance Variables ◽  
Aflatoxin B

The objective of this study was to evaluate the effects of using the Saccharomyces cerevisiae yeast cell wall (YCW) as an aflatoxin B<sub>1 </sub>(AFB<sub>1</sub>) adsorbent in broiler chicken feed on performance and carcass characteristics. The present study used a randomized complete block with four treatments in a 2 (with or without AFB<sub>1</sub>) × 2 (with or without YCW) factorial design. No interaction effect (P &gt; 0.05) between AFB<sub>1</sub> and YCW was found on the studied performance variables. The addition of YCW to the diets stimulated the feed intake of chickens during 1–21 days of age. However, YCW did not significantly increase (P &gt; 0.05) weight gain nor did it change feed conversion. The presence of AFB<sub>1</sub> in the diet did not affect (P &gt; 0.05) performance parameters. The addition of YCW to the feed containing AFB<sub>1</sub> significantly increased (P &lt; 0.05) the post-fasting live weight (781.12 g), chilled carcass weight (554.41 g), and leg weight (163.34 g) compared to feed without AFB<sub>1</sub> and YCW (764.84 g; 533.41 g; 161.88 g), feed with only YCW (764.22 g; 546.87 g; 159.34 g), and feed with only AFB<sub>1</sub> (735.41 g; 510.56 g; 152.75 g). In conclusion, YCW effectively reduced some of the deleterious effects of AFB<sub>1</sub> in broilers.

scholarly journals The b’x Region of Yeast Protein Disulfide Isomerase is Not Essential for Saccharomyces cerevisiae Viability at 30 °C

2009 ◽  
Vol 3 (1) ◽  
pp. 27-32
Author(s):  
PURKAN PURKAN ◽  
LALU RUDYAT TELLY SAVALAS ◽  
MULIAWATI SINDUMARTA ◽  
DESSY NATALIA
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Disulfide Isomerase ◽  
Yeast Protein ◽  
Disulfide Isomerase ◽  
Protein Disulfide

GPI-Modified Proteins Non-covalently Attached to Saccharomyces cerevisiae Yeast Cell Wall

2019 ◽  
Vol 84 (12-13) ◽  
pp. 1513-1520 ◽  
Author(s):  
V. V. Rekstina ◽  
A. A. Bykova ◽  
R. H. Ziganshin ◽  
T. S. Kalebina
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Yeast Cell ◽  
Yeast Cell Wall ◽  
Modified Proteins

The ‘yeast cell wall chip’ – a tool to analyse the regulation of cell wall biogenesis in Saccharomyces cerevisiae

Microbiology ◽  
2005 ◽  
Vol 151 (7) ◽  
pp. 2241-2249 ◽  
Author(s):  
Jose M. Rodríguez-Peña ◽  
Rosa M. Pérez-Díaz ◽  
Sara Alvarez ◽  
Clara Bermejo ◽  
Raúl García ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Yeast Cell ◽  
Transcriptional Activation ◽  
Ease Of Use ◽  
Yeast Cell Wall ◽  
Gene Microarray ◽  
Experimental Conditions ◽  
Wall Functions ◽  
Cell Wall Damage

Within the field of Saccharomyces cerevisiae functional genomics, DNA microarrays have become a very useful tool to study genome-wide gene-expression changes under diverse experimental conditions. Here, the design and production of a gene microarray, called the ‘yeast cell wall chip’, specifically tailored to investigate cell wall functions, is described. This array has been validated and shown to be useful to address gene involvement in the regulation of the response to cell wall damage in yeast. The advantages of this tailored gene microarray, which contains 390 genes, in terms of reproducibility, accuracy, versatility and ease of use are reported. Importantly, the microarray design permits the performance of a double hybridization process (two experiments) on the same slide. Cell wall stress leads to the transcriptional activation of a set of genes involved in cell wall remodelling. This response has been shown to be strongly controlled by the MAP kinase (MAPK) Slt2p, but other signalling pathways have also been suggested to be involved in this process. Here, using the tailored microarray, the role of the HOG1 pathway in the regulation of the transcriptional compensatory response to cell wall damage was evaluated by comparing the transcriptional profiles of a hog1 mutant and a wild-type strain in the presence of Congo red. Two genes, YFL014W (HSP12) and YLR414C, were found to be dependent on the Hog1p MAPK for their induction, indicating that an additional level of regulation of cell wall functions is mediated by this MAPK.

scholarly journals Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly.

1990 ◽  
Vol 110 (5) ◽  
pp. 1833-1843 ◽  
Author(s):  
C Boone ◽  
S S Sommer ◽  
A Hensel ◽  
H Bussey
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Structural Analysis ◽  
Yeast Cell ◽  
Gene Disruption ◽  
Secretory Pathway ◽  
Yeast Cell Wall ◽  
Beta Glucan ◽  
Polymer Size

The Saccharomyces cerevisiae KRE1 gene encodes a Ser/Thr-rich protein, that is directed into the yeast secretory pathway, where it is highly modified, probably through addition of O-linked mannose residues. Gene disruption of the KRE1 locus leads to a 40% reduced level of cell wall (1----6)-beta-glucan. Structural analysis of the (1----6)-beta-glucan fraction, isolated from a strain with a krel disruption mutation, showed that it had an altered structure with a smaller average polymer size. Mutations in two other loci, KRE5 and KRE6 also lead to a defect in cell wall (1----6)-beta-glucan production and appear to be epistatic to KRE1. These findings outline a possible pathway of assembly of yeast cell wall (1----6)-beta-glucan.

Influence of soil microorganisms on the trapping of nematodes by nematophagous fungi

1984 ◽  
Vol 30 (12) ◽  
pp. 1437-1439 ◽  
Author(s):  
W. D. Rosenzweig ◽  
D. Ackroyd
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Yeast Cell ◽  
Soil Microorganisms ◽  
Nematophagous Fungi ◽  
Yeast Cell Wall ◽  
Soil Organisms ◽  
Yeast Saccharomyces Cerevisiae ◽  
Arthrobotrys Conoides

The influence of 29 species of soil organisms (21 bacteria and 8 fungi) on nematode capture by 3 species of nematophagous fungi was investigated. Only the yeast, Saccharomyces cerevisiae, was found to bind to lectin-containing traps of Arthrobotrys conoides and Monacrosporium rutgeriensis and prevent nematode capture. None of the organisms studied bound to the traps of Monacrosporium eudermatum. The trap lectin of Arthrobotrys conoides, being specific for glucose – mannose, apparently binds to the mannan component of the yeast cell wall. At this time, it is not clear why the yeast binds to traps of Monacrosporium rutgeriensis since the trap lectin is highly specific for 2-deoxyglucose, a saccharide that does not appear to occur in nature.

scholarly journals EFFECTS OF LIVE SACCHAROMYCES CEREVISIAE AND YEAST CELL WALL ON PERFORMANCE OF BROILER CHICK

2018 ◽  
Vol 43 (3) ◽  
pp. 57-74
Author(s):  
El-Afifi, T. M. ◽  
Abdel-Aziz, M. F. ◽  
Hemid, A. A. ◽  
Masoud, M. S.
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Yeast Cell ◽  
Yeast Cell Wall ◽  
Broiler Chick

scholarly journals SMA-based Extraction of the Yeast Cell Wall Integrity Sensor Mid2 from Native Membranes of Saccharomyces cerevisiae – Electron Microscopy Study

2019 ◽  
Vol 25 (S2) ◽  
pp. 1338-1339
Author(s):  
Natalia Voskoboynikova ◽  
Maria Karlova ◽  
Rainer Kurre ◽  
Juergen Heinisch ◽  
Heinz-Juergen Steinhoff ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Yeast Cell ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Cell Wall Integrity ◽  
Yeast Cell Wall
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