scholarly journals Field Detection ofSchistosoma japonicumCercariae in Environmental Water Samples by Quantitative PCR

2011 ◽  
Vol 77 (6) ◽  
pp. 2192-2195 ◽  
Author(s):  
Caitlin Worrell ◽  
Ning Xiao ◽  
Jorge E. Vidal ◽  
Lin Chen ◽  
Bo Zhong ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Surface Waters ◽  
Water Samples ◽  
Sampling Methods ◽  
Environmental Water ◽  
Quantitative Detection ◽  
Mouse Bioassay ◽  
The Novel ◽  
Water Sampling ◽  
Species Specific

ABSTRACTA species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection ofS. japonicumin surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria.

scholarly journals Fast UHPLC-MS/MS for the Simultaneous Determination of Azithromycin, Erythromycin, Fluoxetine and Sotalol in Surface Water Samples

2021 ◽  
Vol 11 (18) ◽  
pp. 8316
Author(s):  
Mira Azzi ◽  
Sylvain Ravier ◽  
Assem Elkak ◽  
Bruno Coulomb ◽  
Jean-Luc Boudenne
Keyword(s):  
Surface Water ◽  
Simultaneous Determination ◽  
Surface Waters ◽  
Water Samples ◽  
High Performance ◽  
Chemical Properties ◽  
Environmental Water ◽  
Butyl Ether ◽  
Flight Mass Spectrometry

Chromatographic development for the determination of pharmaceuticals in environmental water samples is particularly challenging when the analytes have significantly different physico-chemical properties (solubility, polarity, pKa) often requiring multiple chromatographic methods for each active component. This paper presents a method for the simultaneous determination of azithromycin, erythromycin (antibiotics), fluoxetine (anti-depressant) and sotalol (b-blocker) in surface waters by ultra-high-performance liquid chromatography coupled with ultra-high-resolution time-of-flight mass spectrometry. These pharmaceuticals—presenting a broad spectrum of polarity (0.24 ≤ log Kow ≤ 4.05)—were separated on a C-18 analytical column, after a simple filtration step for freshwater samples or after a liquid–liquid extraction with Methyl-tertio-butyl ether (MTBE) for seawater samples. The optimized separation method (in terms of nature of column and eluent, elution gradient, and of mass spectrometric parameters), enable one to reach limits of detection ranging between 2 and 7 ng L−1 and limits of quantification between 7 and 23 ng L−1 for the four targeted molecules, within a three minute run. This method was validated using samples collected from three different surface waters in Lebanon (freshwater and seawater) and analytical results were compared with those obtained in surface waters sampled in a French river, equivalent in terms of human activities. Using this method, we report the highest concentration of pharmaceuticals found in surface water (up to 377 ng L−1 and 268 ng L−1, respectively, for azithromycin and erythromycin, in the Litani river, Lebanon).

scholarly journals Methanobrevibacter ruminantium as an Indicator of Domesticated-Ruminant Fecal Pollution in Surface Waters

2007 ◽  
Vol 73 (21) ◽  
pp. 7118-7121 ◽  
Author(s):  
Jennifer A. Ufnar ◽  
Shiao Y. Wang ◽  
David F. Ufnar ◽  
R. D. Ellender
Keyword(s):  
Surface Waters ◽  
Water Samples ◽  
Environmental Samples ◽  
Environmental Water ◽  
Fecal Pollution ◽  
Environmental Water Samples ◽  
Amplification Product ◽  
Methanobrevibacter Ruminantium

ABSTRACT A PCR-based assay (Mrnif) targeting the nifH gene of Methanobrevibacter ruminantium was developed to detect fecal pollution from domesticated ruminants in environmental water samples. The assay produced the expected amplification product only when the reaction mixture contained DNA extracted from M. ruminantium culture, bovine (80%), sheep (100%), and goat (75%) feces, and water samples from a bovine waste lagoon (100%) and a creek contaminated with bovine lagoon waste (100%). The assay appears to be specific and sensitive and can distinguish between domesticated- and nondomesticated-ruminant fecal pollution in environmental samples.

scholarly journals Simultaneous Quantification of Multiple Food- and Waterborne Pathogens by Use of Microfluidic Quantitative PCR

2013 ◽  
Vol 79 (9) ◽  
pp. 2891-2898 ◽  
Author(s):  
Satoshi Ishii ◽  
Takahiro Segawa ◽  
Satoshi Okabe
Keyword(s):  
Quantitative Pcr ◽  
Water Samples ◽  
Simultaneous Detection ◽  
Environmental Water ◽  
Waterborne Pathogens ◽  
Fecal Indicator ◽  
Content Type ◽  
Template Dna ◽  
Multiple Pathogens ◽  
Taqman Qpcr

ABSTRACTThe direct quantification of multiple pathogens has been desired for diagnostic and public health purposes for a long time. In this study, we applied microfluidic quantitative PCR (qPCR) technology to the simultaneous detection and quantification of multiple food- and waterborne pathogens. In this system, multiple singleplex qPCR assays were run under identical detection conditions in nanoliter-volume chambers that are present in high densities on a chip. First, we developed 18 TaqMan qPCR assays that could be run in the same PCR conditions by using prevalidated TaqMan probes. Specific and sensitive quantification was achieved by using these qPCR assays. With the addition of two previously validated TaqMan qPCR assays, we used 20 qPCR assays targeting 10 enteric pathogens, a fecal indicator bacterium (generalEscherichia coli), and a process control strain in the microfluidic qPCR system. We preamplified the template DNA to increase the sensitivity of the qPCR assays. Our results suggested that preamplification was effective for quantifying small amounts of the template DNA without any major impact on the sensitivity, efficiency, and quantitative performance of qPCR. This microfluidic qPCR system allowed us to detect and quantify multiple pathogens from fecal samples and environmental water samples spiked with pathogens at levels as low as 100 cells/liter. These results suggest that the routine monitoring of multiple pathogens in food and water samples is now technically feasible. This method may provide more reliable information for risk assessment than the current fecal contamination indicator approach.

scholarly journals Development of a Quantitative PCR Assay for Arcobacter spp. and its Application to Environmental Water Samples

2018 ◽  
Vol 33 (3) ◽  
pp. 309-316 ◽  
Author(s):  
Rajani Ghaju Shrestha ◽  
Yasuhiro Tanaka ◽  
Bikash Malla ◽  
Sarmila Tandukar ◽  
Dinesh Bhandari ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Water Samples ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Pcr Assay ◽  
Quantitative Pcr Assay

Detection of the European pond turtle (Emys orbicularis) by environmental DNA: is eDNA adequate for reptiles?

2018 ◽  
Vol 39 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Matthieu Raemy ◽  
Sylvain Ursenbacher
Keyword(s):  
Water Samples ◽  
Environmental Dna ◽  
Population Monitoring ◽  
Environmental Water ◽  
Visual Observation ◽  
Water Sampling ◽  
Emys Orbicularis ◽  
Pond Turtle ◽  
Artificial Ponds ◽  
Specific Species

Abstract Recent studies have demonstrated the potential of combining molecular technologies with environmental sampling to detect various vertebrate species in aquatic ecosystems. The European pond turtle (Emys orbicularis) is a threatened and elusive aquatic reptile with shy behaviour. We aimed to develop and evaluate a methodology to detect the presence of this secretive aquatic reptile in ponds from environmental water samples. First, we determined that reptilian DNA can be isolated and amplified from water samples in artificial and natural ponds with known turtle density. Then we compared the potential of two water sampling methods (through filtration or precipitation) and found no significant differences between these approaches. Finally, we demonstrated that the eDNA concentration detected is not correlated with the number of E. orbicularis individuals or biomass. Detection of eDNA was higher in artificial ponds with small volumes of water or in the shallow waters of natural ponds. The eDNA-based methodology aims to detect the presence of specific species, even at low density, with better accuracy than visual observation. However, our study indicates that this method of population monitoring should be applied with caution to aquatic reptiles.

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