scholarly journals Chronic Helicobacter pylori Infection Does Not Significantly Alter the Microbiota of the Murine Stomach

2006 ◽  
Vol 73 (3) ◽  
pp. 1010-1013 ◽  
Author(s):  
Mai Ping Tan ◽  
Maria Kaparakis ◽  
Maja Galic ◽  
John Pedersen ◽  
Martin Pearse ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Microbial Composition ◽  
H Pylori ◽  
The Impact ◽  
Gastric Microbiota ◽  
Antigenic Load ◽  
Restriction Fragment Length

ABSTRACT We examined the impact of Helicobacter pylori infection on the murine gastric microbiota by culture and terminal-restriction fragment length polymorphism and found that neither acute nor chronic H. pylori infection substantially affected the gastric microbial composition. Interestingly, the total H. pylori burden detected by real-time PCR was significantly higher than that revealed by viable counts, suggesting that the antigenic load sustaining H. pylori-induced gastritis could be considerably higher than previously believed.

scholarly journals Rare Incidence of Interspousal Transmission ofHelicobacter pylori in Asymptomatic Individuals in Japan

1999 ◽  
Vol 37 (12) ◽  
pp. 4174-4176 ◽  
Author(s):  
Junko Suzuki ◽  
Hiroe Muraoka ◽  
Intetsu Kobayasi ◽  
Toshiro Fujita ◽  
Tetsuya Mine
Keyword(s):  
Helicobacter Pylori ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Electrophoretic Patterns ◽  
H Pylori ◽  
Asymptomatic Individuals ◽  
Restriction Fragment Length

PCR-restriction fragment length polymorphism electrophoretic patterns of amplified ureB and ureC ofHelicobacter pylori were compared between spouses after digestion with restriction endonucleases. Twenty of 21 couples, both members of which were positive for H. pylori, showedureB and ureC patterns that differed between spouses. We concluded that in Japan, interspousal transmission ofH. pylori occurs rarely.

scholarly journals PCR Using 3′-Mismatched Primers To Detect A2142C Mutation in 23S rRNA Conferring Resistance to Clarithromycin in Helicobacter pylori Clinical Isolates

2000 ◽  
Vol 38 (2) ◽  
pp. 923-925 ◽  
Author(s):  
Teresa Alarcón ◽  
Diego Domingo ◽  
Nuria Prieto ◽  
Manuel López-Brea
Keyword(s):  
Helicobacter Pylori ◽  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
23S Rrna ◽  
Rrna Gene ◽  
Specific Primer ◽  
23S Rrna Gene ◽  
Agar Dilution ◽  
Restriction Fragment Length

Twenty-five clarithromycin-resistant Helicobacter pylori strains (selected by agar dilution) were studied to detect A2142G and A2143G mutations in the 23S rRNA gene by a PCR-restriction fragment length polymorphism method and an A2142C mutation by PCR using a 3′-mismatched specific primer. A 700-bp amplified fragment was obtained by the mismatched PCR only in strains without an A2142G or A2143G mutation, indicating that those strains had the A2142C mutation.

scholarly journals Comparison of PCR-Restriction Fragment Length Polymorphism Analysis and PCR-Direct Sequencing Methods for Differentiating Helicobacter pylori ureB Gene Variants

2000 ◽  
Vol 38 (1) ◽  
pp. 165-169
Author(s):  
Toshihito Tanahashi ◽  
Masakazu Kita ◽  
Tadashi Kodama ◽  
Naoki Sawai ◽  
Yoshio Yamaoka ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Helicobacter Pylori ◽  
Amino Acid ◽  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Direct Sequencing ◽  
Base Change ◽  
Pcr Rflp ◽  
Restriction Fragment Length

ABSTRACT A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the Helicobacter pylori genes is widely used to differentiate strains. However, with this typing method only a single base change at a specific restriction site can be detected. In addition, it is unclear whether the nucleotide base change recognized by RFLP is related to a substitution of encoded amino acid. To examine the validity of the PCR-RFLP method, 933-bp PCR products were obtained from 41 different clinical H. pylori isolates and were digested with Sau 3A restriction endonuclease. Furthermore, the nucleotides of the same region in the ureB gene were directly sequenced and compared. PCR-RFLP confirmed that there was genetic diversity within the ureB gene with three distinct types, one being well conserved and the other two being variations. However, the direct sequencing method revealed that there was no difference at the nucleotide level among these RFLP types. Base substitutions recognized by Sau 3A occurred in the third-base position and did not change the encoded amino acid. In addition, many nucleotide mutations, which could not be recognized by Sau 3A, were frequently found. These results suggest that the PCR-RFLP method provides for an easy typing scheme of isolates, but does not reveal the true extent of genetic diversity. It is proposed that careful observation is required for the interpretation of results when clinical isolates are differentiated.

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