Use of Green Fluorescent Protein To Monitor Cell Envelope Stress in Lactococcus lactis
2009 ◽
Vol 76
(3)
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pp. 978-981
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Keyword(s):
Dot Blot
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ABSTRACT A Lactococcus lactis reporter system suitable to detect cell envelope stress in high-throughput settings was developed by fusing the CesR-regulated promoter of llmg0169 to the gfpuv gene. A dot blot assay allowed fast detection of green fluorescent protein (GFP) fluorescence even at low production levels. Unexpectedly, this promoter was also induced by mitomycin C via CesR.
2003 ◽
Vol 69
(7)
◽
pp. 4214-4218
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2017 ◽
Vol 33
(11)
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2011 ◽
Vol 2011
(3)
◽
pp. 243-248
Keyword(s):
2005 ◽
Vol 71
(5)
◽
pp. 2338-2346
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