scholarly journals Distinct Patterns of Picocyanobacterial Communities in Winter and Summer in the Chesapeake Bay

2010 ◽  
Vol 76 (9) ◽  
pp. 2955-2960 ◽  
Author(s):  
Haiyuan Cai ◽  
Kui Wang ◽  
Sijun Huang ◽  
Nianzhi Jiao ◽  
Feng Chen
Keyword(s):  
16S Rrna ◽  
Chesapeake Bay ◽  
Internal Transcribed Spacer ◽  
Specific Primers ◽  
Changing Environments ◽  
Internal Transcribed Spacer Sequences ◽  
Different Populations ◽  
Cluster A ◽  
Sense And Respond ◽  
16S Rrna Clone Libraries

ABSTRACT In the Chesapeake Bay, picocyanobacteria were usually 100-fold less abundant in winter than in summer. However, little is known about how picocyanobacterial populations shift between winter and summer in the bay. This is due mainly to undetectable winter picocyanobacterial populations in bacterial 16S rRNA clone libraries. In this study, the winter and summer picocyanobacterial populations in the bay were detected using picocyanobacterium-specific primers and were compared based on the analysis of rRNA internal transcribed spacer sequences. Temperature was found to be the dominant environmental factor controlling picocyanobacterial populations in the Chesapeake Bay. In the summer, marine cluster B Synechococcus dominated the upper bay, while a unique cluster, CB1 (marine cluster A [MC-A] Synechococcus), made up the vast majority in the middle and lower bay. In the winter, the picocyanobacteria shifted to completely different populations. Subclades CB6 and CB7, which belong to MC-A Synechococcus and Cyanobium, respectively, made up the entire winter picocyanobacterial populations in the bay. Interestingly, the winter members in subclade CB6 clustered closely with Synechococcus CC9311, a coastal strain known to have a greater capacity to sense and respond to changing environments than oceanic strains.

scholarly journals Diverse and Unique Picocyanobacteria in Chesapeake Bay, Revealed by 16S-23S rRNA Internal Transcribed Spacer Sequences

2006 ◽  
Vol 72 (3) ◽  
pp. 2239-2243 ◽  
Author(s):  
Feng Chen ◽  
Kui Wang ◽  
Jinjun Kan ◽  
Marcelino T. Suzuki ◽  
K. Eric Wommack
Keyword(s):  
Chesapeake Bay ◽  
Internal Transcribed Spacer ◽  
23S Rrna ◽  
Internal Transcribed Spacer Sequences ◽  
Cluster A

ABSTRACT rRNA internal transcribed spacer phylogeny showed that Chesapeake Bay is populated with diverse Synechococcus strains, including members of the poorly studied marine cluster B. Marine cluster B prevailed in the upper bay, while marine cluster A was common in the lower bay. Interestingly, marine cluster B Synechococcus included phycocyanin- and phycoerythrin-rich strains.

scholarly journals Influence of Molecular Resolution on Sequence-Based Discovery of Ecological Diversity amongSynechococcusPopulations in an Alkaline Siliceous Hot Spring Microbial Mat

2010 ◽  
Vol 77 (4) ◽  
pp. 1359-1367 ◽  
Author(s):  
Melanie C. Melendrez ◽  
Rachel K. Lange ◽  
Frederick M. Cohan ◽  
David M. Ward
Keyword(s):  
16S Rrna ◽  
Internal Transcribed Spacer ◽  
Microbial Mats ◽  
Hot Spring ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
23S Rrna ◽  
Ecological Diversity ◽  
Protein Encoding ◽  
Internal Transcribed Spacer Sequences

ABSTRACTPrevious research has shown that sequences of 16S rRNA genes and 16S-23S rRNA internal transcribed spacer regions may not have enough genetic resolution to define all ecologically distinctSynechococcuspopulations (ecotypes) inhabiting alkaline, siliceous hot spring microbial mats. To achieve higher molecular resolution, we studied sequence variation in three protein-encoding loci sampled by PCR from 60°C and 65°C sites in the Mushroom Spring mat (Yellowstone National Park, WY). Sequences were analyzed using the ecotype simulation (ES) and AdaptML algorithms to identify putative ecotypes. Between 4 and 14 times more putative ecotypes were predicted from variation in protein-encoding locus sequences than from variation in 16S rRNA and 16S-23S rRNA internal transcribed spacer sequences. The number of putative ecotypes predicted depended on the number of sequences sampled and the molecular resolution of the locus. Chao estimates of diversity indicated that few rare ecotypes were missed. Many ecotypes hypothesized by sequence analyses were different in their habitat specificities, suggesting different adaptations to temperature or other parameters that vary along the flow channel.

scholarly journals The Termite Group I Phylum Is Highly Diverse and Widespread in the Environment

2007 ◽  
Vol 73 (20) ◽  
pp. 6682-6685 ◽  
Author(s):  
Daniel P. R. Herlemann ◽  
Oliver Geissinger ◽  
Andreas Brune
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Specific Primers ◽  
Environmental Distribution ◽  
Data Set ◽  
Group I

ABSTRACT The bacterial candidate phylum Termite Group I (TG-1) presently consists mostly of “Endomicrobia,” which are endosymbionts of flagellate protists occurring exclusively in the hindguts of termites and wood-feeding cockroaches. Here, we show that public databases contain many, mostly undocumented 16S rRNA gene sequences from other habitats that are affiliated with the TG-1 phylum but are only distantly related to “Endomicrobia.” Phylogenetic analysis of the expanded data set revealed several diverse and deeply branching lineages comprising clones from many different habitats. In addition, we designed specific primers to explore the diversity and environmental distribution of bacteria in the TG-1 phylum.

Variation in bacterial endosymbionts associated with the date palm hopper,Ommatissus lybicuspopulations

2017 ◽  
Vol 108 (2) ◽  
pp. 271-281 ◽  
Author(s):  
S. Karimi ◽  
H. Izadi ◽  
M. Askari Seyahooei ◽  
A. Bagheri ◽  
P. Khodaygan
Keyword(s):  
Date Palm ◽  
Multiple Infections ◽  
Infection Frequency ◽  
Pcr Assay ◽  
Specific Primers ◽  
Consensus Sequences ◽  
Pcr Products ◽  
Bacterial Endosymbionts ◽  
Different Populations ◽  
Polymerase Chain

AbstractThe date palm hopper,Ommatissus lybicus, is a key pest of the date palm, which is expected to be comprised of many allopatric populations. The current study was carried out to determine bacterial endosymbiont diversity in the different populations of this pest. Ten date palm hopper populations were collected from the main date palm growing regions in Iran and an additional four samples from Pakistan, Oman, Egypt and Tunisia for detection of primary and secondary endosymbionts using polymerase chain reaction (PCR) assay with their specific primers. The PCR products were directly sequenced and edited using SeqMan software. The consensus sequences were subjected to a BLAST similarity search. The results revealed the presence of ‘CandidatusSulcia muelleri’ (primary endosymbiont) andWolbachia,ArsenophonusandEnterobacter(secondary endosymbionts) in all populations. This assay failed to detect ‘CandidatusNasuia deltocephalinicola’ andSerratiain these populations. ‘Ca. S. muelleri’ exhibited a 100% infection frequency in populations andWolbachia,ArsenophonusandEnterobacterdemonstrated 100, 93.04 and 97.39% infection frequencies, respectively. The infection rate ofArsenophonusandEnterobacterranged from 75 to 100% and 62.5 to 100%, respectively, in different populations of the insect. The results demonstrated multiple infections by ‘Ca. Sulcia muelleri’,Wolbachia,ArsenophonusandEnterobacterin the populations and may suggest significant roles for these endosymbionts on date palm hopper population fitness. This study provides an insight to endosymbiont variation in the date palm hopper populations; however, further investigation is needed to examine how these endosymbionts may affect host fitness.

scholarly journals Case Report. Internal transcribed spacer sequence based molecular confirmation and drug efficacy assessment against Toxascaris leonina (Linstow, 1909) infection in Asiatic lions (Panthera leo persica)

2017 ◽  
Vol 54 (2) ◽  
pp. 152-156
Author(s):  
A. D. Moudgil ◽  
L. D. Singla ◽  
M. P. Singh
Keyword(s):  
Body Weight ◽  
Internal Transcribed Spacer ◽  
Drug Efficacy ◽  
Extended Period ◽  
Dose Schedule ◽  
Faecal Egg Count ◽  
Efficacy Assessment ◽  
Internal Transcribed Spacer Sequences ◽  
Zoological Park ◽  
Toxascaris Leonina

Summary The eggs recovered during faecal screening of Asiatic lions (kept at MC Zoological Park, Punjab, India) were delineated as Toxascaris leonina eggs based on morphometric and molecular studies (polymerase chain reaction targeting internal transcribed spacer sequences). Therapeutic management with fenbendazole @10 mg/kg body weight, once daily orally for three consecutive days proved ineffective with maximum faecal egg count reduction (FECR) on day 3 post treatments (69.35 %). But, therapeutic intervention with extended period dose schedule (5 consecutive days) with fenbendazole (@10 mg/kg body weight) proved effective and showed a maximum FECR of 95.34 % at day 7 post treatments. But, when ivermectin (@100μg/kg body weight) was given orally on three alternate days, proved effective as FECR of 95.74 % was recorded at day 7 post treatments. Thus, present study highlights the molecular confi rmation of T. leonina and its management using fenbendazole and ivermectin in Asiatic lions.

Sign in / Sign up

Export Citation Format

Share Document