Use of Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy To Identify Microbial Metabolic Products on Carbonate Mineral Surfaces
2008 ◽
Vol 74
(14)
◽
pp. 4553-4559
◽
Keyword(s):
E Coli
◽
ABSTRACT This paper demonstrates the use of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to detect microbial metabolic products on carbonate mineral surfaces. By creating an ATR-FTIR spectral database for specific organic acids using ATR-FTIR spectroscopy we were able to distinguish metabolic acids on calcite surfaces following Escherichia coli growth. The production of these acids by E. coli was verified using high-performance liquid chromatography with refractive index detection. The development of this technique has allowed us to identify microbial metabolic products on carbonate surfaces in nutrient-limited cave environments.
2000 ◽
Vol 64
(5)
◽
pp. 666-672
◽
2007 ◽
Vol 70
(1-3)
◽
pp. 87-94
◽
1999 ◽
Vol 63
(18)
◽
pp. 2709-2725
◽
2018 ◽
Vol 12
(2)
◽
pp. 355-370
◽
2020 ◽
Vol 107
◽
pp. 103033
◽