scholarly journals Identification of Low-Molecular-Weight Nucleic Acid-Related Substances Secreted by Streptomyces aureofaciens

1983 ◽  
Vol 46 (3) ◽  
pp. 762-764
Author(s):  
Alírio De Carvalho ◽  
Rubens Molinari
Keyword(s):  
Molecular Weight ◽  
Nucleic Acid ◽  
Low Molecular Weight ◽  
Streptomyces Aureofaciens ◽  
Related Substances

scholarly journals Effect of kinetin on nucleic acid synthesis in senescing and young leaves in Brassica oleracea L. var. gongylodes L.

2015 ◽  
Vol 44 (4) ◽  
pp. 553-565
Author(s):  
J. Legocka ◽  
A. Szweykowska
Keyword(s):  
Molecular Weight ◽  
Nucleic Acid ◽  
Acid Synthesis ◽  
Inhibitory Effect ◽  
Rrna Synthesis ◽  
Low Molecular Weight ◽  
Chlorophyll B ◽  
Mature Leaves ◽  
Young Leaves ◽  
Brassica Oleracea L

In detached kohlrabi leaves senescing in the dark, the decrease in chlorophyll to was more pronounced than in chlorophyll a. The retardation by kinetin of the chlorophyll loss was also markedly stronger in the case of chlorophyll b. Using the fractionation of nucleic acids on polyacrylamide gels it has been shown that during leaf senescence the level of all RNA species decreased, whereas the amount of DNA was more or less constant. In the presence of kinetin, the loss of RNA was inhibited and the incorporation of precursor into the cytoplasmic rRNA as well as into low molecular weight RNA species was supported. Chloroplast rRNA synthesis has not been detected in mature leaves and kinetin showed no effect in this respect. In young expanding leaves detached and kept in light, the synthesis of cytoplasmic rRNA was strongly stimulated by kinetin, whereas in the case of Chloroplast rRNA only an inhibitory effect of kinetin could be found. The results suggest that the cytokinins are primarily involved in processes of the synthesis of cytoplasmic rRNA and low molecular RNA fractions, and in this way affect the development of plastids, in particular the course of their senescence.

Direct Isolation of High-Quality Low Molecular Weight RNA of Pear Peel from the Extraction Mixture Containing Nucleic Acid

2009 ◽  
Vol 44 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Xin-wei Wang ◽  
Ai-sheng Xiong ◽  
Quan-hong Yao ◽  
Zhen Zhang ◽  
Yu-shan Qiao
Keyword(s):  
Molecular Weight ◽  
Nucleic Acid ◽  
Low Molecular Weight ◽  
High Quality ◽  
Extraction Mixture

scholarly journals Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum polynucleotide phosphorylase

1971 ◽  
Vol 121 (4) ◽  
pp. 613-620 ◽  
Author(s):  
Pearl I. Peterkin ◽  
P. S. Fitt
Keyword(s):  
Molecular Weight ◽  
Ionic Strength ◽  
Nucleic Acid ◽  
Halophilic Bacteria ◽  
High Ionic Strength ◽  
Low Molecular Weight ◽  
Polynucleotide Phosphorylase

1. Polynucleotide phosphorylase was purified 200-fold from Halobacterium cutirubrum. 2. It is membrane-associated and can be solubilized by sonication. 3. The purified enzyme requires a high ionic strength for both stability and activity. 4. It is Mn2+-dependent, has all three typical polynucleotide phosphorylase activities and is specific for nucleoside diphosphates. 5. The enzyme is of low molecular weight.

scholarly journals Synergistic Effect of Low Molecular Weight Polyethylenimine and Polyethylene Glycol Components in Dynamic Nonviral Vector Structure, Toxicity, and Transfection Efficiency

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1460 ◽  
Author(s):  
Bogdan Florin Craciun ◽  
Gabriela Gavril ◽  
Dragos Peptanariu ◽  
Laura Elena Ursu ◽  
Lilia Clima ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Nucleic Acid ◽  
Transfection Efficiency ◽  
Morphological Data ◽  
Low Molecular Weight ◽  
Nonviral Vectors ◽  
Main Challenge ◽  
Vector Structure ◽  
Imine Bond

When studying polyethylenimine derivatives as nonviral vectors for gene delivery, among the important issues to be addressed are high toxicity, low transfection efficiency, and nucleic acid polyplex condensation. The molecular weight of polyethylenimine, PEGylation, biocompatibility and, also, supramolecular structure of potential carrier can all influence the nucleic acid condensation behavior, polyplex size, and transfection efficiency. The main challenge in building an efficient carrier is to find a correlation between the constituent components, as well as the synergy between them, to transport and to release, in a specific manner, different molecules of interest. In the present study, we investigated the synergy between components in dynamic combinatorial frameworks formed by connecting PEGylated squalene, poly-(ethyleneglycol)-bis(3-aminopropyl) and low molecular weight polyethylenimine components to 1,3,5-benzenetrialdehyde, via reversible imine bond, applying a dynamic combinatorial chemistry approach. We report comparative structural and morphological data, DNA binding affinity, toxicity and transfection efficiency concerning the ratio of polyethylenimine and presence or absence of poly-(ethyleneglycol)-bis(3-aminopropyl) in composition of dynamic combinatorial frameworks. In vitro biological assessments have revealed the fact that nonviral vectors containing poly-(ethyleneglycol)-bis(3-aminopropyl) and the lowest amount of polyethylenimine have significant transfection efficiency at N/P 50 ratio and display insignificant cytotoxicity on the HeLa cell line.

Development of DNA aptamers targeting low-molecular-weight amyloid-β peptide aggregatesin vitro

2018 ◽  
Vol 54 (36) ◽  
pp. 4593-4596 ◽  
Author(s):  
Madhuri Chakravarthy ◽  
Hadi AlShamaileh ◽  
He Huang ◽  
Rudi K. Tannenberg ◽  
Suxiang Chen ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Nucleic Acid ◽  
Amyloid Β ◽  
Low Molecular Weight ◽  
Dna Aptamers ◽  
Amyloid Β Peptide ◽  
Aβ Peptide ◽  
Peptide Fragments ◽  
Alzheimer Brain

We have developed a novel functional nucleic acid aptamer to amyloid-β peptide 1–40 (Aβ1–40) and investigated its potential to detect Aβ peptide fragments in neuropathologically confirmed Alzheimer brain hippocampus tissues samples.

Sign in / Sign up

Export Citation Format

Share Document