scholarly journals Characterization of two genes encoding Bacillus thuringiensis insecticidal crystal proteins toxic to Coleoptera species.

1992 ◽  
Vol 58 (12) ◽  
pp. 3921-3927 ◽  
Author(s):  
W P Donovan ◽  
M J Rupar ◽  
A C Slaney ◽  
T Malvar ◽  
M C Gawron-Burke ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Crystal Proteins ◽  
Genes Encoding ◽  
Insecticidal Crystal Proteins

scholarly journals Diversity of Insecticidal Crystal proteins (ICPs) of indigenous Bacillus thuringiensis strains

2018 ◽  
Vol 5 ◽  
pp. 11-18
Author(s):  
Ganga G.C. ◽  
Charu Arjya ◽  
Yamuna Khadka ◽  
Sabina Dhamala
Keyword(s):  
Bacillus Thuringiensis ◽  
Slight Modification ◽  
Soil Samples ◽  
Colony Morphology ◽  
Selective Enrichment ◽  
Crystal Proteins ◽  
Selection Protocol ◽  
White Type ◽  
Insecticidal Crystal Proteins

Objectives: The purpose of this study was to characterize the indigenous Bacillus thuringiensis (Bt) isolated from the soil samples of central development region of Terai. Methods: A total of 50 soil samples collected from cultivated and barren fields of Terai region. Isolation was carried out using the acetate selection protocol as described by (Russell and Al 1987) with a slight modification. The Nutrient broth (NB) was acetated by using 0.25M sodium acetate which is a selective enrichment method for isolation of Bt. Characterization of the isolate was done by phenotyping methods (microscopy and biochemical). Results: No distinct variation was observed between the isolates of cultivable and uncultivable lands. Bt were categorized into7 different types based on colony morphology. The dominant colony was fried egg type identical with the reference strain, followed by flat white type of colony. The result showed that even though the colony morphology is same but the ICPs (Insecticidal crystal proteins) shapes produced by them vary, rod shapes (53.57%), spherical (10.71%), ovoid (8.3%), amorphous (17.85%), capheaded (9.5%). ICPs morphology reveal the cry1, cry2, cry3, cry4, cry8, cry 9, cry10 and cry11 types of gene may be present in the native isolates. Conclusion: This study represents the first report of several indigenous Bacillus thuringiensis strains with significantly different ICPs producing stains from hot tropical climate.

scholarly journals New Strategy for Identification of Novel cry-Type Genes from Bacillus thuringiensis Strains

2005 ◽  
Vol 71 (2) ◽  
pp. 761-765 ◽  
Author(s):  
Corina M. Berón ◽  
Leonardo Curatti ◽  
Graciela L. Salerno
Keyword(s):  
Bacillus Thuringiensis ◽  
Phylogenetic Distance ◽  
Cloning And Sequencing ◽  
Degenerate Primers ◽  
Dna Templates ◽  
Cry Proteins ◽  
New Strategy ◽  
Genes Encoding ◽  
Encoding Protein

ABSTRACT We designed five degenerate primers for detection of novel cry genes from Bacillus thuringiensis strains. An efficient strategy was developed based on a two-step PCR approach with these primers in five pair combinations. In the first step, only one of the primer pairs is used in the PCR, which allows amplification of DNA fragments encoding protein regions that include consensus domains of representative proteins belonging to different Cry groups. A second PCR is performed by using the first-step amplification products as DNA templates and the set of five primer combinations. Cloning and sequencing of the last-step amplicons allow both the identification of known cry genes encoding Cry proteins covering a wide phylogenetic distance and the detection and characterization of cry-related sequences from novel B. thuringiensis isolates.

Membrane binding of insecticidal crystal proteins of Bacillus thuringiensis

2000 ◽  
Vol 28 (5) ◽  
pp. A438-A438
Author(s):  
Hiroshi Sakai ◽  
Tohru Komano ◽  
Masashi Yamagiwa
Keyword(s):  
Bacillus Thuringiensis ◽  
Membrane Binding ◽  
Crystal Proteins ◽  
Insecticidal Crystal Proteins

Characterization of Baculovirus Insecticides Expressing Tailored Bacillus thuringiensis CryIA(b) Crystal Proteins

1995 ◽  
Vol 66 (3) ◽  
pp. 249-257 ◽  
Author(s):  
John W.M. Martens ◽  
Marga Knoester ◽  
Franci Weijts ◽  
Sander J.A. Groffen ◽  
Zhihong Hu ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Crystal Proteins

Larvicidal toxins fromBacillusthuringiensissubspp.kurstaki,morrisoni(straintenebrionis), andisraelensishave no microbicidal or microbiostatic activity against selected bacteria, fungi, and algae in vitro

2002 ◽  
Vol 48 (3) ◽  
pp. 262-267 ◽  
Author(s):  
J Koskella ◽  
G Stotzky
Keyword(s):  
Bacillus Thuringiensis ◽  
Mixed Culture ◽  
Gram Positive ◽  
Gram Negative ◽  
Crystal Proteins ◽  
Growth Of A Variety ◽  
Insecticidal Toxins ◽  
Antibiotic Effect ◽  
Insecticidal Crystal Proteins

The insecticidal toxins from Bacillus thuringiensis subspp. kurstaki (antilepidopteran), morrisoni strain tenebrionis (anticoleopteran), and israelensis (antidipteran) did not affect the growth of a variety of bacteria (8 gram-negative, 5 gram-positive, and a cyanobacterium), fungi (2 Zygomycetes, 1 Ascomycete, 2 Deuteromycetes, and 2 yeasts), and algae (primarily green and diatoms) in pure and mixed culture, as determined by dilution, disk-diffusion, and sporulation assays with purified free and clay-bound toxins. The insecticidal crystal proteins from B. thuringiensis subspp. kurstaki and israelensis had no antibiotic effect on various gram-positive bacteria.Key words: insecticidal toxins, Bacillus thuringiensis, microbiostatic, microbicidal.

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