Bacterial canker is a devastating disease of kiwifruit caused by the bacterium <i>Pseudomonas syringe</i> pv. <i>actinidiae</i>. Canker disease of kiwifruit in Korea has been controlled using streptomycin for more than two decades. Four streptomycin-resistant strains, belonging to biovar 2, which are found only in Korea, were collected between 2013 and 2014 from different orchards located in Jeju, Korea. The genetic background for streptomycin resistance among <i>P. syringe</i> pv. <i>actinidiae</i> strains were determined by examining the presence of <i>strA</i>-<i>strB</i> or <i>aadA</i>, which are genes frequently found in streptomycin-resistant bacteria, and a point mutation at codon 43 in the <i>rpsL</i> gene. All four streptomycin-resistant strains of <i>P. syringe</i> pv. <i>actinidiae</i> investigated in this study contained <i>strA</i>-<i>strB</i> as a resistant determinant. The presence of the <i>aadA</i> gene and a mutation in codon 43 of the <i>rpsL</i> gene was not identified.
Ecological and genetic analysis of copper and streptomycin resistance in Pseudomonas syringae pv. syringae.
