scholarly journals Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products.

1993 ◽  
Vol 59 (7) ◽  
pp. 2161-2165 ◽  
Author(s):  
B Wegmüller ◽  
J Lüthy ◽  
U Candrian
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Dairy Products ◽  
Raw Milk ◽  
Campylobacter Coli ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Direct Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Milk And Dairy Products

scholarly journals Survey of chicken abattoir for the presence of Campylobacter jejuni and Campylobacter coli

2006 ◽  
Vol 48 (6) ◽  
pp. 307-310 ◽  
Author(s):  
Ana L.L. Cortez ◽  
Angela C.F.B. Carvalho ◽  
Eliana Scarcelli ◽  
Simone Miyashiro ◽  
Ana M.C. Vidal-Martins ◽  
...  
Keyword(s):  
Public Health ◽  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Intestinal Tract ◽  
Campylobacter Coli ◽  
Improve Quality ◽  
Chain Reaction ◽  
Paulo State ◽  
Polymerase Chain ◽  
Campylobacter Spp

The genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhea. These species may be found in water, food and in the intestinal tract of chickens. This study investigated the presence of Campylobacter jejuni and Campylobacter coli in chicken abattoirs in São Paulo State, Brazil. A total of 288 samples of feces, feathers, scald water, evisceration water, chiller water, and the rinse water of eviscerated, not eviscerated and chilled carcasses were collected in six chicken abattoirs. Polymerase Chain Reaction (PCR) was performed in Campylobacter spp.-positive isolates using the gene HIP, specific for hippuricase enzyme from Campylobacter jejuni and aspartokinase gene, specific to detect Campylobacter coli. The percentage of positive isolates of Campylobacter jejuni was 4.9% (14/288). Isolation was greater in feces samples (22%, 8/36). One sample was positive for the species C. coli. In conclusion, the results indicate that it is necessary to improve quality control for Campylobacter spp. in chicken abattoirs.

scholarly journals Characterization of Campylobacter jejuni An Important Zoonotic Food Borne Pathogen from Mastitis Milk and Raw Milk Samples

2021 ◽  
Author(s):  
Sumedha Bobade ◽  
K. Vijayarani ◽  
K.G. Tirumurugaan ◽  
A. Thangavelu ◽  
S. Vairamuthu
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Morphological Characteristics ◽  
Raw Milk ◽  
Phenotypic Characterization ◽  
Chain Reaction ◽  
Milk Samples ◽  
Food Borne ◽  
Polymerase Chain ◽  
Pcr Targeting

Background: Campylobacter has emerged as an important zoonotic food borne pathogen of human and animals worldwide. Campylobacter is one of the most common bacterial enteropathogens of food borne origin in industrialized countries with C. jejuni being the most common species followed by C. coli. There are very few cases reported from mastitis therefore this study was aimed to determine the incidence of Campylobacter jejuni from from mastitis milk and raw milk samples. Methods: Total of 72 milk samples comprising mastitis milk (20) and raw milk (52) were collected. The samples were subjected to cultural examination, biochemical as well as molecular identification. The isolates were further subjected to phenotypic characterization by biochemical test and genotypic characterization by Polymerase Chain Reaction. The isolates were subjected to PCR targeting hip O and MAP A genes. Result: The 52 samples showed growth on modified Blood Free Charcoal Cefoperazone Deoxycholate agar media and 18 (34.61%) samples showed typical morphological characteristics. The result revealed that 10 (19.23%) isolates were positive by phenotypic characteristic and 7(70%) by Polymerase chain reaction for C. jejuni. The outcome result showed that importance of Campylobacter jejuni in cattle, especially raw milk and milk from mastitis cows, as a potential source for transmission of Campylobacteriosis in human and dairy farm environment. This can cause acute bacterial gastroenteritis in humans and associated with foodborn infection, food safety and a serious public health threat.

Detection of Campylobacter jejuni and Campylobacter coli in Foods by Enrichment Culture and Polymerase Chain Reaction Enzyme-Linked Immunosorbent Assay

2002 ◽  
Vol 65 (5) ◽  
pp. 760-767 ◽  
Author(s):  
F. J. BOLTON ◽  
A. D. SAILS ◽  
A. J. FOX ◽  
D. R. A. WAREING ◽  
D. L. A. GREENWAY
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Predictive Value ◽  
Enrichment Culture ◽  
Food Samples ◽  
Campylobacter Coli ◽  
Chain Reaction ◽  
Enrichment Cultures ◽  
Selective Agar ◽  
Polymerase Chain

A polymerase chain reaction (PCR) assay based on a solution hybridization format with colorimetric end-point detection (PCR ELISA) was investigated for the specific detection of Campylobacter jejuni and Campylobacter coli in food samples following enrichment culture. One hundred fifteen samples of raw meat and offal (poultry, porcine, ovine, and bovine), raw shellfish, and artificially contaminated milk were enriched in blood-free Campylobacter Enrichment Broth for 48 h. Enrichment cultures were subcultured to Campylobacter blood-free selective agar plates, and presumptive isolates were identified by phenotypic methods. DNA was extracted from 1-ml aliquots of the enrichment cultures using a rapid extraction method, and the DNA was used as the template in a PCR ELISA. A comparison of the PCR ELISA with the enrichment culture and subculture to selective agar method showed that the results of 112 of the 115 samples tested were in agreement by both methods. Seventy-one of the various food samples were positive in the PCR ELISA, and 70 samples were positive by culture. The PCR ELISA had a sensitivity of 99% and a specificity of 96%, with a positive predictive value of 97% and a negative predictive value of 98%. The PCR ELISA is a rapid, sensitive, and specific method for the detection of C. jejuni and C. coli in foods following enrichment culture and significantly reduces the time required for their detection.

Sign in / Sign up

Export Citation Format

Share Document