scholarly journals Localization and Characterization of α-Glucosidase Activity in Lactobacillus brevis

1994 ◽  
Vol 60 (9) ◽  
pp. 3074-3078 ◽  
Author(s):  
S. De Cort ◽  
H. M. C. Shantha Kumara ◽  
H. Verachtert
2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Fang Fang ◽  
Jie Xu ◽  
Qiaoyu Li ◽  
Xiaoxuan Xia ◽  
Guocheng Du

2018 ◽  
Vol 107 ◽  
pp. 874-881 ◽  
Author(s):  
Marion E. Fraunhofer ◽  
Andreas J. Geissler ◽  
Daniel Wefers ◽  
Mirko Bunzel ◽  
Frank Jakob ◽  
...  
Keyword(s):  

2017 ◽  
Vol 31 (3) ◽  
pp. 544-553 ◽  
Author(s):  
Sheng Hu ◽  
Xiang Zhang ◽  
Yi Lu ◽  
Yue-Cheng Lin ◽  
Dong-Fang Xie ◽  
...  

1964 ◽  
Vol 10 (5) ◽  
pp. 757-762 ◽  
Author(s):  
M. M. Hauser ◽  
R. E. Smith

Data for 59 lactobacilli isolated from Canadian Cheddar cheese and 9 named species, previously characterized on the basis of morphological, cultural, and biochemical features, were analyzed by the Adansonian numerical methods of Sneath. Results confirmed the validity of groups, originally designated as Lactobacillus plantarum, Lactobacillus casei, Lactobacillus brevis, and Lactobacillus fermenti types. The computer technique provided numerical estimates of strain and group relationships, emphasizing the extreme heterogeneity of the groups.


Microbiology ◽  
2009 ◽  
Vol 155 (5) ◽  
pp. 1726-1737 ◽  
Author(s):  
Takaomi Wada ◽  
Masafumi Noda ◽  
Fumi Kashiwabara ◽  
Hyung Joon Jeon ◽  
Ayano Shirakawa ◽  
...  

In this study we isolated over 250 lactic acid bacteria (LAB) candidates from fruit, flowers, vegetables and a fermented food to generate an LAB library. One strain, designated 925A, isolated from kimchi (a traditional Korean fermented dish made from Chinese cabbage) produced a novel type of bacteriocin, brevicin 925A, which is effective against certain LAB, including strains of Lactobacillus, Enterococcus, Streptococcus, Bacillus and Listeria. Strain 925A, identified as Lactobacillus brevis, harboured at least four plasmids and we determined the entire nucleotide sequence of each one. The four plasmids were designated pLB925A01–04, and have molecular sizes of 1815, 3524, 8881 and 65 037 bp, respectively. We obtained bacteriocin non-producing derivatives by treatment of strain 925A with novobiocin. All of these derivatives, which were susceptible to their own antibacterial product, lost the largest plasmid, pLB925A04, suggesting that the genes for bacteriocin biosynthesis (breB and breC) and immunity (breE) are located on pLB925A04. The partial amino acid sequence of purified brevicin 925A and sequence analysis of pLB925A04 showed that breB is the structural gene for brevicin 925A. We constructed a shuttle vector (pLES003, 6134 bp) that can replicate in both Escherichia coli and LAB such as Lactobacillus plantarum, Lb. brevis, Lactobacillus helveticus, Lactobacillus hilgardii and Enterococcus hirae. To determine the function of gene breE, which displays no significant similarity to any other sequences in the blast search database, the gene was inserted into pLES003. A pLB925A04-cured derivative transformed with pLES003 carrying breE acquired immunity to brevicin 925A, suggesting that breE encodes an immunity protein.


2018 ◽  
Vol 15 (3) ◽  
pp. 737-746
Author(s):  
Guetouache Mourad ◽  
Guessas Bettache

Morphological, physiological and biochemical characteristics were employed to identify lactic acid bacteria (LAB), isolated from traditional (butter) was collected from different rural areas of the province of Djelfa. Among 177 isolates, 79 lactic acid bacterial (LAB) strains were isolated and purified. The results obtained show that the isolates obtained belong to the following genus Lactobacillus, Lactococcus, Enterococci and Leuconostoc characterize the biodiversity of this traditional butter studied. Only Gram-positive and catalase negative isolates were identified at species level. The most common LAB belonging to the species Lactobacillus alimentarius (15.19 %), Lactobacillus plantarum (22.78 %), Lactobacillus fermentum (18.99 %), Lactobacillus brevis (06.33 %), Lactococcus lactis (12.66 %), Lactococcus cremoris (06.33 %), Leuconostoc mesenteroides (06.33 %) and Enterococcus faecalis (11.39 %). The samples pH average was 6.06 ± 0.34, microbiological analysis results were; total mesophilic aerobic flora (TMAF) (2, 22 ± 0, 68).10 3cfu/ml, total coliforms 0,54 ± 0.56 ufc/ml, fecal coliforms 0,6 ± 0.50 cfu/ml, yeast (0,48 ± 0.31). 10 cfu/ml, Staphylococcus aureus, Salmonella and moulds weren’t detected.


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