scholarly journals Phanerochaete chrysosporiumCellobiohydrolase and Cellobiose Dehydrogenase Transcripts in Wood

1998 ◽  
Vol 64 (5) ◽  
pp. 1924-1928 ◽  
Author(s):  
Marcelo A. Vallim ◽  
Bernard J. H. Janse ◽  
Jill Gaskell ◽  
Aline A. Pizzirani-Kleiner ◽  
Daniel Cullen
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Cellulose Degradation ◽  
Cellobiose Dehydrogenase ◽  
Wood Chips ◽  
Defined Media

ABSTRACT The transcripts of structurally related cellobiohydrolase genes inPhanerochaete chrysosporium-colonized wood chips were quantified. The transcript patterns obtained were dramatically different from the transcript patterns obtained previously in defined media. Cellobiose dehydrogenase transcripts were also detected, which is consistent with the hypothesis that such transcripts play an important role in cellulose degradation.

Oxidative cellulose degradation by cellobiose dehydrogenase from Phanerochaete chrysosporium: A model compound study

Holzforschung ◽  
2005 ◽  
Vol 59 (3) ◽  
pp. 263-268 ◽  
Author(s):  
Martin Kruså ◽  
Gunnar Henriksson ◽  
Gunnar Johansson ◽  
Torbjörn Reitberger ◽  
Helena Lennholm
Keyword(s):  
Hydrogen Peroxide ◽  
Molecular Oxygen ◽  
Phanerochaete Chrysosporium ◽  
Hydroxyl Radicals ◽  
Gluconic Acid ◽  
Model Compound ◽  
Cellulose Degradation ◽  
Extracellular Enzyme ◽  
Cellobiose Dehydrogenase ◽  
Cellulose Depolymerization

AbstractCellobiose dehydrogenase (CDH) is an extracellular enzyme produced by various wood-degrading fungi. It oxidizes cellobiose to cellobionolactone under reduction of molecular oxygen to hydrogen peroxide, and Fe3+to Fe2+. These activated agents can thereafter form highly reactive hydroxyl radicals, which depolymerize wood polymers. In this work, cellulose depolymerization by CDH was studied using a model compound, methyl β-D-glucopyranoside. The formation of glucose, arabinose, gluconic acid, erythrulose and formaldehyde were detected and a mechanism for the reaction is proposed. The biological importance of this enzyme-initiated reaction is discussed.

scholarly journals Expression of Phanerochaete chrysosporium Genes Encoding Lignin Peroxidases, Manganese Peroxidases, and Glyoxal Oxidase in Wood

1998 ◽  
Vol 64 (9) ◽  
pp. 3536-3538 ◽  
Author(s):  
Bernard J. H. Janse ◽  
Jill Gaskell ◽  
Masood Akhtar ◽  
Daniel Cullen
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Peroxidase ◽  
Ligninolytic Enzymes ◽  
Wood Chips ◽  
Mrna Levels ◽  
Defined Media ◽  
Magnetic Capture ◽  
Lignin Peroxidases ◽  
Genes Encoding ◽  
Glyoxal Oxidase

ABSTRACT Expression of Phanerochaete chrysosporium genes encoding ligninolytic enzymes was assessed in wood. Poly(A) RNA was extracted from colonized wood chips by magnetic capture, and specific transcripts were quantified by competitive reverse transcriptase PCR. mRNA levels varied substantially among lignin peroxidase genes, and transcript patterns were dramatically different from those in previous studies with defined media.

Substrate specificity of cellobiose dehydrogenase from Phanerochaete chrysosporium

1998 ◽  
Vol 1383 (1) ◽  
pp. 48-54 ◽  
Author(s):  
Gunnar Henriksson ◽  
Veljo Sild ◽  
István J Szabó ◽  
Göran Pettersson ◽  
Gunnar Johansson
Keyword(s):  
Substrate Specificity ◽  
Phanerochaete Chrysosporium ◽  
Cellobiose Dehydrogenase

scholarly journals Comparative Transcriptome and Secretome Analysis of Wood Decay Fungi Postia placenta and Phanerochaete chrysosporium

2010 ◽  
Vol 76 (11) ◽  
pp. 3599-3610 ◽  
Author(s):  
Amber Vanden Wymelenberg ◽  
Jill Gaskell ◽  
Michael Mozuch ◽  
Grzegorz Sabat ◽  
John Ralph ◽  
...  
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Cellulose Degradation ◽  
Expression Patterns ◽  
Wood Decay ◽  
Brown Rot ◽  
White Rot ◽  
Glycosyl Hydrolases ◽  
Decay Fungi ◽  
Postia Placenta ◽  
Wood Decay Fungi

ABSTRACT Cellulose degradation by brown rot fungi, such as Postia placenta, is poorly understood relative to the phylogenetically related white rot basidiomycete, Phanerochaete chrysosporium. To elucidate the number, structure, and regulation of genes involved in lignocellulosic cell wall attack, secretome and transcriptome analyses were performed on both wood decay fungi cultured for 5 days in media containing ball-milled aspen or glucose as the sole carbon source. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), a total of 67 and 79 proteins were identified in the extracellular fluids of P. placenta and P. chrysosporium cultures, respectively. Viewed together with transcript profiles, P. chrysosporium employs an array of extracellular glycosyl hydrolases to simultaneously attack cellulose and hemicelluloses. In contrast, under these same conditions, P. placenta secretes an array of hemicellulases but few potential cellulases. The two species display distinct expression patterns for oxidoreductase-encoding genes. In P. placenta, these patterns are consistent with an extracellular Fenton system and include the upregulation of genes involved in iron acquisition, in the synthesis of low-molecular-weight quinones, and possibly in redox cycling reactions.

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