Oxidative cellulose degradation by cellobiose dehydrogenase from Phanerochaete chrysosporium: A model compound study

Holzforschung ◽  
2005 ◽  
Vol 59 (3) ◽  
pp. 263-268 ◽  
Author(s):  
Martin Kruså ◽  
Gunnar Henriksson ◽  
Gunnar Johansson ◽  
Torbjörn Reitberger ◽  
Helena Lennholm
Keyword(s):  
Hydrogen Peroxide ◽  
Molecular Oxygen ◽  
Phanerochaete Chrysosporium ◽  
Hydroxyl Radicals ◽  
Gluconic Acid ◽  
Model Compound ◽  
Cellulose Degradation ◽  
Extracellular Enzyme ◽  
Cellobiose Dehydrogenase ◽  
Cellulose Depolymerization

AbstractCellobiose dehydrogenase (CDH) is an extracellular enzyme produced by various wood-degrading fungi. It oxidizes cellobiose to cellobionolactone under reduction of molecular oxygen to hydrogen peroxide, and Fe3+to Fe2+. These activated agents can thereafter form highly reactive hydroxyl radicals, which depolymerize wood polymers. In this work, cellulose depolymerization by CDH was studied using a model compound, methyl β-D-glucopyranoside. The formation of glucose, arabinose, gluconic acid, erythrulose and formaldehyde were detected and a mechanism for the reaction is proposed. The biological importance of this enzyme-initiated reaction is discussed.

scholarly journals Superoxide dismutase enhances the formation of hydroxyl radicals in the reaction of 3-hydroxyanthranilic acid with molecular oxygen

1988 ◽  
Vol 251 (3) ◽  
pp. 893-899 ◽  
Author(s):  
H Iwahashi ◽  
T Ishii ◽  
R Sugata ◽  
R Kido
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Hydroxyl Radical ◽  
Molecular Oxygen ◽  
Hydroxyl Radicals ◽  
Fenton Reaction ◽  
Potassium Phosphate ◽  
Radical Formation ◽  
Superoxide Anions ◽  
Hydroxyanthranilic Acid

Superoxide dismutase (SOD) enhanced the formation of hydroxyl radicals, which were detected by using the e.s.r. spin-trapping technique, in a reaction mixture containing 3-hydroxyanthranilic acid (or p-aminophenol), Fe3+ ions, EDTA and potassium phosphate buffer, pH 7.4. The hydroxyl-radical formation enhanced by SOD was inhibited by catalase and desferrioxamine, and stimulated by EDTA and diethylenetriaminepenta-acetic acid, suggesting that both hydrogen peroxide and iron ions participate in the reaction. The hydroxyl-radical formation enhanced by SOD may be considered to proceed via the following steps. First, 3-hydroxyanthranilic acid is spontaneously auto-oxidized in a process that requires molecular oxygen and yields superoxide anions and anthranilyl radicals. This reaction seems to be reversible. Secondly, the superoxide anions formed in the first step are dismuted by SOD to generate hydrogen peroxide and molecular oxygen, and hence the equilibrium in the first step is displaced in favour of the formation of superoxide anions. Thirdly, hydroxyl radicals are generated from hydrogen peroxide through the Fenton reaction. In this Fenton reaction Fe2+ ions are available since Fe3+ ions are readily reduced by 3-hydroxyanthranilic acid. The superoxide anions do not seem to participate in the reduction of Fe3+ ions, since superoxide anions are rapidly dismuted by SOD present in the reaction mixture.

Solar water treatment: principles and reactors

1997 ◽  
Vol 35 (4) ◽  
pp. 137-148 ◽  
Author(s):  
Roland Goslich ◽  
Ralf Dillert ◽  
Detlef Bahnemann
Keyword(s):  
Hydrogen Peroxide ◽  
Water Treatment ◽  
Molecular Oxygen ◽  
Model Compound ◽  
Degradation Process ◽  
Dichloroacetic Acid ◽  
Solar Water ◽  
Treatment Principles ◽  
Advantages And Disadvantages ◽  
Different Types

Solar detoxification of dichloroacetic acid (DCA) as a model compound was studied. Different types of reactors, either light-concentrating or non-concentrating were used and their advantages and disadvantages were compared and discussed. The influence of hydrogen peroxide and molecular oxygen on the degradation process was studied with this model compound.

scholarly journals Phanerochaete chrysosporiumCellobiohydrolase and Cellobiose Dehydrogenase Transcripts in Wood

1998 ◽  
Vol 64 (5) ◽  
pp. 1924-1928 ◽  
Author(s):  
Marcelo A. Vallim ◽  
Bernard J. H. Janse ◽  
Jill Gaskell ◽  
Aline A. Pizzirani-Kleiner ◽  
Daniel Cullen
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Cellulose Degradation ◽  
Cellobiose Dehydrogenase ◽  
Wood Chips ◽  
Defined Media

ABSTRACT The transcripts of structurally related cellobiohydrolase genes inPhanerochaete chrysosporium-colonized wood chips were quantified. The transcript patterns obtained were dramatically different from the transcript patterns obtained previously in defined media. Cellobiose dehydrogenase transcripts were also detected, which is consistent with the hypothesis that such transcripts play an important role in cellulose degradation.

scholarly journals RuIII(edta) complexes as molecular redox catalysts in chemical and electrochemical reduction of dioxygen and hydrogen peroxide: inner-sphere versus outer-sphere mechanism

RSC Advances ◽  
2021 ◽  
Vol 11 (35) ◽  
pp. 21359-21366
Author(s):  
Debabrata Chatterjee ◽  
Marta Chrzanowska ◽  
Anna Katafias ◽  
Maria Oszajca ◽  
Rudi van Eldik
Keyword(s):  
Hydrogen Peroxide ◽  
Electron Transfer ◽  
Electrochemical Reduction ◽  
Molecular Oxygen ◽  
Outer Sphere ◽  
Transfer Processes ◽  
Edta Complexes ◽  
Inner Sphere ◽  
Electron Transfer Processes ◽  
Sphere Mechanism

[RuII(edta)(L)]2–, where edta4– =ethylenediaminetetraacetate; L = pyrazine (pz) and H2O, can reduce molecular oxygen sequentially to hydrogen peroxide and further to water by involving both outer-sphere and inner-sphere electron transfer processes.

Removal of Proteinaceous Soils Using Hydroxyl Radicals Generated by the Electrolysis of Hydrogen Peroxide

2002 ◽  
Vol 250 (2) ◽  
pp. 409-414 ◽  
Author(s):  
Koreyoshi Imamura ◽  
Yoichiro Tada ◽  
Hirohumi Tanaka ◽  
Takaharu Sakiyama ◽  
Kazuhiro Nakanishi
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radicals
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