scholarly journals Pulsed-Electric Field Treatment Enhances the Bactericidal Action of Nisin against Bacillus cereus

2000 ◽  
Vol 66 (1) ◽  
pp. 428-430 ◽  
Author(s):  
Irene E. Pol ◽  
Hennie C. Mastwijk ◽  
Paul V. Bartels ◽  
Eddy J. Smid
Keyword(s):  
Electric Field ◽  
Bacillus Cereus ◽  
Pulsed Electric Field ◽  
Low Doses ◽  
Bactericidal Action ◽  
Vegetative Cells ◽  
Pulsed Electric Field Treatment ◽  
Electric Field Treatment

ABSTRACT Vegetative cells of Bacillus cereus were subjected to low doses of nisin (0.06 μg/ml) and mild pulsed-electric field treatment (16.7 kV/cm, 50 pulses each of 2-μs duration). Combining both treatments resulted in a reduction of 1.8 log units more than the sum of the reductions obtained with the single treatments, indicating synergy.

scholarly journals Sensitivities of Germinating Spores and Carvacrol-Adapted Vegetative Cells and Spores of Bacillus cereus to Nisin and Pulsed-Electric-Field Treatment

2001 ◽  
Vol 67 (4) ◽  
pp. 1693-1699 ◽  
Author(s):  
Irene E. Pol ◽  
Willy G. C. van Arendonk ◽  
Hennie C. Mastwijk ◽  
Judit Krommer ◽  
Eddy J. Smid ◽  
...  
Keyword(s):  
Electric Field ◽  
Bacillus Cereus ◽  
Treatment Resistance ◽  
Pulsed Electric Field ◽  
The Other ◽  
Heat Sensitivity ◽  
Vegetative Cells ◽  
Germination Process ◽  
Pulsed Electric Field Treatment ◽  
Electric Field Treatment

ABSTRACT Treatment of Bacillus cereus spores with nisin and/or pulsed-electric-field (PEF) treatment did not lead to direct inactivation of the spores or increased heat sensitivity as a result of sublethal damage. In contrast, germinating spores were found to be sensitive to PEF treatment. Nisin treatment was more efficient than PEF treatment for inactivating germinating spores. PEF resistance was lost after 50 min of germination, and not all germinated spores could be inactivated. Nisin, however, was able to inactivate the germinating spores to the same extent as heat treatment. Resistance to nisin was lost immediately when the germination process started. A decrease in the membrane fluidity of vegetative cells caused by incubation in the presence of carvacrol resulted in a dramatic increase in the sensitivity to nisin. On the other hand, inactivation by PEF treatment or by a combination of nisin and PEF treatments did not change after adaptation to carvacrol. Spores grown in the presence of carvacrol were not susceptible to nisin and/or PEF treatment in any way.

scholarly journals The Electrode Modality Development in Pulsed Electric Field Treatment Facilitates Biocellular Mechanism Study and Improves Cancer Ablation Efficacy

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Chao Cen ◽  
Xinhua Chen
Keyword(s):  
Electric Field ◽  
Laser Scanning ◽  
Specific Treatment ◽  
Pulsed Electric Field ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Invasive Technique ◽  
Bipolar Electrode ◽  
Pulsed Electric Field Treatment ◽  
Electric Field Treatment

Pulsed electric field treatment is now widely used in diverse biological and medical applications: gene delivery, electrochemotherapy, and cancer therapy. This minimally invasive technique has several advantages over traditional ablation techniques, such as nonthermal elimination and blood vessel spare effect. Different electrodes are subsequently developed for a specific treatment purpose. Here, we provide a systematic review of electrode modality development in pulsed electric field treatment. For electrodes invented for experiment in vitro, sheet electrode and electrode cuvette, electrodes with high-speed fluorescence imaging system, electrodes with patch-clamp, and electrodes with confocal laser scanning microscopy are introduced. For electrodes invented for experiment in vivo, monopolar electrodes, five-needle array electrodes, single-needle bipolar electrode, parallel plate electrodes, and suction electrode are introduced. The pulsed electric field provides a promising treatment for cancer.

Pulsed electric field treatment of Lacticaseibacillus rhamnosus and Lacticaseibacillus paracasei, bacteria with probiotic potential

LWT ◽  
2021 ◽  
pp. 112304
Author(s):  
Aleksandra Djukić-Vuković ◽  
Saša Haberl Meglič ◽  
Karel Flisar ◽  
Ljiljana Mojović ◽  
Damijan Miklavčič
Keyword(s):  
Electric Field ◽  
Pulsed Electric Field ◽  
Probiotic Potential ◽  
Pulsed Electric Field Treatment ◽  
Electric Field Treatment

scholarly journals Influence of electrophysical treatment on oilseed crops structure with Х-ray micro tomography application

2018 ◽  
Vol 80 (3) ◽  
pp. 116-123
Author(s):  
I. A. Shorstkii ◽  
D. A. Khudyakov
Keyword(s):  
Electric Field ◽  
Porous Structure ◽  
Diffusion Processes ◽  
Material Model ◽  
Pulsed Electric Field ◽  
Microwave Treatment ◽  
Bearing Materials ◽  
Micro Tomography ◽  
Pulsed Electric Field Treatment ◽  
Electric Field Treatment

Morphological capillary-porous structure analysis of the oilseed materials, using x-ray microtomography in the longitudinal and transverse section and FESEM analysis of the surface microstructure after electrical and microwave treatment presented in current paper. Experimental data were obtained on the basis of the Institute of Materials Research and Engineering (Singapore). Two types of treatment considered: non-thermal pulsed electric field treatment, creating an electroporation effect of the oilseeds structure and microwave treatment. The main characteristic of capillary-porous structure of oil-bearing materials is given. Local changes in the electron density of the oilseed object under study, during the passage of radiation, made it possible to determine clearly the air cavities in the structure of the sunflower nucleus. The influence of a pulsed electric field treatment on the integrity of the structure of oil-cell membranes has been obtained with the creation of a material that has a greater permeability for diffusion processes. Experimentally was determined that over 2500 electric pores were formed on an area of 1 sq.cm as a result of a pulsed electric field treatment. In the case of a pulsed electric field treatment, the oil body material model can be represented as a capillary model with capillary and electroporation radii, thus expanding the model of a bidispersed structure with the addition micro capillaries, formed by an electric field. The data obtained are of interest not only for the technology of processing oilseeds, but also for the analysis methods of new electrophysical treatments.

Sign in / Sign up

Export Citation Format

Share Document