scholarly journals Cloning and Sequencing of an Alkaline Protease Gene from Bacillus lentus and Amplification of the Gene on the B. lentus Chromosome by an Improved Technique

2000 ◽  
Vol 66 (2) ◽  
pp. 825-827 ◽  
Author(s):  
Per Linå Jørgensen ◽  
Martin Tangney ◽  
Poul Erik Pedersen ◽  
Sven Hastrup ◽  
Børge Diderichsen ◽  
...  
Keyword(s):  
Alkaline Protease ◽  
Protease Gene ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Bacillus Lentus ◽  
Amplification Technique ◽  
Alkaline Protease Gene ◽  
Cloned Gene ◽  
Improved Technique ◽  
Alkalophilic Bacillus

ABSTRACT A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.

scholarly journals Molecular Characterization of Alkaline Protease Gene Isolated from Aeromonas hydrophila strain AH10

2020 ◽  
Vol 8 (2) ◽  
pp. 154-160
Author(s):  
N.G. Ramesh Babu ◽  
Anupa Mary Aji
Keyword(s):  
Aeromonas Hydrophila ◽  
Alkaline Protease ◽  
Recovery Process ◽  
Protease Gene ◽  
Gene Encoding ◽  
Silver Recovery ◽  
Ph Optimum ◽  
Protease Enzyme ◽  
Alkaline Protease Gene

  Alkaline protease enzymes are enzymes which can catalyze the process of proteolysis between the pH ranges from 8 to 12.  Extracellular alkaline proteases are used as additives in detergent powders. In the present study, source of the organism was from a detergent contaminated area. The study has been carried out in Aeromonas hydrophila AH10 strain that produces protease enzyme with an alkaline pH optimum. The organism was a gram-negative rod with a protease enzyme activity of 0.385 ml/min. purification of the protease enzyme from the bacteria was carried out. This protease is suitable for use in alkaline detergent powders as well as in silver recovery process. The Aeromonas hydrophila strain AH10 gene encoding this high-alkaline protease was cloned and characterized.   Int. J. Appl. Sci. Biotechnol. Vol 8(2): 154-160

Using 16S rDNA as Target Site for Homologous Recombination to Improve the Alkaline Protease Production of Bacillus alcalophilus

2014 ◽  
Vol 886 ◽  
pp. 349-354
Author(s):  
Qing Shan Mo ◽  
Yao Tian ◽  
Hui Tu Zhang ◽  
Ling Jun Bu ◽  
Fu Ping Lu
Keyword(s):  
16S Rdna ◽  
Alkaline Protease ◽  
Genetic Manipulation ◽  
Protease Production ◽  
Protease Gene ◽  
Wild Type ◽  
Recombinant Strains ◽  
Manipulation System ◽  
Rdna Sites ◽  
Alkaline Protease Gene

Bacillus alcalophilusisolated was used for the production of alkaline protease. The enzyme encoded by alkaline protease gene (apr4) gene. To further improve the production of the strain for industrial requirement, a genetic manipulation system forBacillus alcalophiluswas developed. Additional copies of theapr4 gene were transferred into the strainBacillus alcalophilusand integrated into the 16S rDNA sites, yielding a series of recombinant strains. One of these recombinant strains, designated K23, exhibited superior properties for production of alkaline protease. the protease activity of K23 achieved by (6.19 ± 0.34) × 104U/ml, which is approximately 111.3% higher than that of the wild-type ones for 50-h fermentation. In addition, the new strain was genetically stable for more than 100 generations. These superior characteristics make it to be more suitable than the wild-type strain for alkaline protease production.

scholarly journals Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene.

1991 ◽  
Vol 57 (4) ◽  
pp. 901-909 ◽  
Author(s):  
J C van der Laan ◽  
G Gerritse ◽  
L J Mulleners ◽  
R A van der Hoek ◽  
W J Quax
Keyword(s):  
Alkaline Protease ◽  
Protease Gene ◽  
Chromosomal Integration ◽  
Alkaline Protease Gene
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