scholarly journals Characterization of Urease-Positive Thermophilic Campylobacter Subspecies by Multilocus Enzyme Electrophoresis Typing

2003 ◽  
Vol 69 (6) ◽  
pp. 3308-3310 ◽  
Author(s):  
Motoo Matsuda ◽  
Aki Kaneko ◽  
Timothy Stanley ◽  
B. Cherie Millar ◽  
M. Miyajima ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis ◽  
Thermophilic Campylobacter ◽  
Variation Data ◽  
Quantitative Analyses ◽  
The Mean ◽  
Campylobacter Lari ◽  
Reference Strains

ABSTRACT Thirty-one urease-positive thermophilic Campylobacter (UPTC) isolates, including three reference strains (NCTC12892, NCTC12895 and NCTC12896), and three Campylobacter lari isolates, which were isolated from several countries and sources, were compared genotypically by using multilocus enzyme electrophoresis (MLEE). We examined allelic variation around seven enzyme loci, including the adenylate kinase, alkaline phosphatase, catalase, fumarase, malic enzyme, malate dehydrogenase, and l-phenylalanyl-l-leucine peptidase loci. MLEE typing revealed the presence of 23 different electrophoretic types (ETs) among the 31 UPTC isolates, and 14 isolates shared six electrophoretic profiles. Three different ETs were identified for the three C. lari isolates examined, and no ETs were shared by UPTC and C. lari isolates. Quantitative analyses were subsequently performed by using allelic variation data, and the results demonstrated that the mean genetic diversity was 0.655. In conclusion, MLEE demonstrated that the UPTC isolates examined are genetically hypervariable and form a cluster separate from the C. lari cluster.

Application of Multilocus Enzyme Electrophoresis to Epidemiologic Investigations ofXanthomonas Maltophilia

1991 ◽  
Vol 12 (3) ◽  
pp. 163-167 ◽  
Author(s):  
Barbara Schable ◽  
Margarita E. Villarino ◽  
Martin S. Favero ◽  
J. Michael Miller
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Epidemiological Data ◽  
Case Definition ◽  
Enzyme Electrophoresis ◽  
Typing Method ◽  
Multilocus Enzyme Electrophoresis ◽  
General Community ◽  
Suspected Outbreak

AbstractObjective:To test the utility of a newly developed multilocus enzyme electrophoresis typing method for Xanthomonas maltophilia.Design:Isolates were first screened by slide agglutination, which served as the standard to characterize the outbreak strains. All isolates were then subjected to multilocus enzyme elec-trophoresis and the results analyzed based on epidemiological data.Setting:This outbreak occurred in a shock-trauma intensive care unit of a large general community hospital.Patients:Patients admitted to the shock-trauma intensive care unit who had X maltophilia isolated from any site > 24 hours after admission met the case definition. Specimens from patients who fit the case definition were characterized, as were specimens from other patients that were used as controls for nonoutbreak isolates. Environmental samples were also evaluated for X maltophilia.Results:Most of the 64 isolates received during this outbreak were serotype 10, and when they were subjected to multilocus enzyme electro-phoresis, one electrophoretic type predominated and correlated to most outbreak isolates. Unrelated isolates of serotype 10 from other institutions all exhibited unique electrophoretic types.Conclusion:Application of multilocus enzyme electrophoresis to X maltophilia outbreaks is a valuable addition to the characterization of suspected outbreak strains.

scholarly journals Genetic analysis ofClavibacter toxicus, the agent of annual ryegrass toxicity

1996 ◽  
Vol 117 (2) ◽  
pp. 393-400 ◽  
Author(s):  
M. S. Johnston ◽  
S. S. Sutherland ◽  
C. C. Constantine ◽  
D. J. Hampsons
Keyword(s):  
Genetic Distances ◽  
The Other ◽  
Enzyme Electrophoresis ◽  
Annual Ryegrass ◽  
Field Isolates ◽  
Multilocus Enzyme Electrophoresis ◽  
Related Group ◽  
Western Australian ◽  
Reference Strains ◽  
Annual Ryegrass Toxicity

SummaryMultilocus enzyme electrophoresis was used to examine the relatedness of 52 isolates ofClavibacter toxicus, the agent of annual ryegrass toxicity. These included 37 Western Australian (WA) field isolates sampled in 3 distinct locations over a 2-year period, and 15 isolates sampled from 6 different host plant species in 3 states in Australia over approximately 8 years. Seventeen reference strains for the related generaCurtobacterium, RhodococcusandArthrobacterwere examined for comparison. The 69 isolates were divided into 29 electrophoretic types (ETs), separated by genetic distances of 0·06 to 0·81. TheC. toxicusisolates fell into 12 ETs, 11 of which formed a tightly clustered group separated by a genetic distance of 0·23 or less. Thirty-one of the WA field isolates ofC. toxicusfell into a single ET, and four into another ET.Clavibacter toxicustherefore formed a closely related group which was genetically distinct from the other plant pathogenic species, and a dominant widely disseminated strain of the species was identified in WA.

scholarly journals Molecular Epidemiology of Recent Belgian Isolates of Neisseria meningitidis Serogroup B

1998 ◽  
Vol 36 (10) ◽  
pp. 2828-2834 ◽  
Author(s):  
M. Van Looveren ◽  
P. Vandamme ◽  
M. Hauchecorne ◽  
M. Wijdooghe ◽  
F. Carion ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Gel Electrophoresis ◽  
Epidemiological Data ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Clin Microbiol Infect ◽  
Epidemic Clone ◽  
Multilocus Enzyme Electrophoresis ◽  
Repetitive Motif ◽  
Reference Strains

In Belgium an increase in the incidence of meningococcal disease has been noted since the early 1990s. Four hundred twenty clinical strains isolated during the period from 1990 to 1995, along with a set of 30 European reference strains, and 20 Dutch isolates were examined by random-primer and repetitive-motif-based PCR. A subset was investigated by multilocus enzyme electrophoresis and pulsed-field gel electrophoresis. The data were compared with results obtained by serotyping (M. Van Looveren, F. Carion, P. Vandamme, and H. Goossens, Clin. Microbiol. Infect. 4:224–228, 1998). Both phenotypic and molecular epidemiological data suggest that the lineage III ofNeisseria meningitidis, first encountered in The Netherlands in about 1980, has been introduced in Belgium. The epidemic clone, as defined by oligonucleotide D8635-primed PCR, encompasses mainly phenotypes B:4:P1.4 and B:nontypeable:P1.4, but strains with several other phenotypes were also encountered. Therefore, serotyping alone would underestimate the prevalence of the epidemic clone.

scholarly journals Genetic relationships amongNeisseria gonorrhoeaeserovars analysed by multilocus enzyme electrophoresis

1992 ◽  
Vol 108 (1) ◽  
pp. 31-38 ◽  
Author(s):  
C. L. Poh ◽  
J. C. Ocampo ◽  
G. K. Loh
Keyword(s):  
Genetic Diversity ◽  
Neisseria Gonorrhoeae ◽  
Genetic Relatedness ◽  
Genetic Relationships ◽  
Electrophoretic Analysis ◽  
Enzyme Electrophoresis ◽  
Enzyme Locus ◽  
Multilocus Enzyme Electrophoresis ◽  
Enzyme Loci ◽  
The Mean

Multilocus enzyme electrophoretic analysis was employed to assess the genetic relatedness ofNeisseria gonorrhoeae. Based on the diversity of electromorphs at 9 enzyme loci, 16 electrophoretic types (ETs) were estabilished amongst the 65 isolates. The average number of alleles per enzyme locus was 1·7 and the mean genetic diversity per locus was 0·212. The majority of isolates belonged to either ET1 (32·3%) or ET2 (16·9%). No specific correlation of ETs was seen with serovars as the major types, ETs 1 and 2, were found distributed amongst the various serovars. Major serovars such as Bacjk (IB-1/2) and Bajk (IB-3/6) were each represented by 6 or 8 ETs respectively. Analysis of the genetic relationships of ETs to each other showed some clustering of subgroups that were more closely related than others.

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