scholarly journals IgA Antibody Response of Swine to Foot-and-Mouth Disease Virus Infection and Vaccination

2010 ◽  
Vol 17 (4) ◽  
pp. 550-558 ◽  
Author(s):  
Juan M. Pacheco ◽  
John E. Butler ◽  
Jessica Jew ◽  
Geoffrey S. Ferman ◽  
James Zhu ◽  
...  
Keyword(s):  
Antibody Response ◽  
Disease Virus ◽  
Control Measure ◽  
Natural Infection ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Local Immunity ◽  
Mouth Disease Virus ◽  
Foot And Mouth

ABSTRACT Foot-and-mouth disease virus (FMDV) continues to be a significant economic problem worldwide. Control of the disease involves the use of killed-virus vaccines, a control measure developed decades ago. After natural infection, the primary site of replication of FMDV is the pharyngeal area, suggesting that a mucosal immune response is the most effective. Humoral immunity to killed-virus vaccination induces antibodies that can prevent the clinical disease but not local infection. Determining whether infection or vaccination stimulates IgA-mediated local immunity depends on the method of analysis. Different assays have been described to analyze the quality of antibody responses of cattle and swine to FMDV, including indirect double-antibody sandwich enzyme-linked immunosorbent assay (IDAS-ELISA) and antibody capture assay-ELISA (ACA-ELISA). We tested these assays on swine and show that vaccinated animals had FMDV-specific IgM and IgG but no IgA in either serum or saliva. After the infection, both assays detected FMDV-specific IgM, IgG, and IgA in serum. Notably, serum IgA was more readily detected using the ACA-ELISA, whereas IgA was not detected in saliva with this assay. FMDV-specific IgA antibodies were detected in saliva samples using the IDAS-ELISA. These data show that parenterally administered, killed-virus vaccine does not induce a mucosal antibody response to FMDV and illuminates limitations and appropriate applications of the two ELISAs used to measure FMDV-specific responses. Further, the presence of the IgA antivirus in serum correlates with the presence of such antibodies in saliva.

scholarly journals Seroprevalence of foot and mouth disease (FMD) among sedentary cattle in northern Plateau, Nigeria

2015 ◽  
Vol 1 (2) ◽  
pp. 169-174 ◽  
Author(s):  
Yiltawe Simwal Wungak ◽  
Ishola Olayinka Olabisi ◽  
Babasola Oluseyi Olugasa ◽  
David Dazhia Lazarus ◽  
Hussaini Gulak Ularamu
Keyword(s):  
Risk Factors ◽  
Whole Blood ◽  
Animal Movement ◽  
Control Measure ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Adult Cattle ◽  
Mouth Disease Virus ◽  
Foot And Mouth

This study was aimed to determine the seroprevalence of foot and mouth disease (FMD) in cattle and identifying the potential risk factors associated with the disease among sedentary cattle in northern part of Plateau state, Nigeria. Two hundred and seventy cattle aged from 6 months to ?3 years old were randomly selected and identified and whole blood collected from the jugular vein using plain evacuated tubes. Whole blood was processed and separated and sera were screened for foot and mouth disease virus (FMDV) 3D non-structural proteins using blocking enzyme linked immunosorbent assay (ELISA). Overall, 55.9% (95%CI: 49.96-61.77) FMD seroprevalence was obtained from the study area. Seroprevalence was highest in Riyom (82.5%), followed by Barkin Ladi (66.2%), Jos South (55.5%) and Bassa (41.2%) (x2 = 17.21, P<0.05). Risk factors for age, management system and location were significant associated (P<0.05) with seroprevalence of FMD. However, there was no significant association with sex (P>0.05). The prevalence odd ratio of FMD was more in Riyom than in Jos South, Barkin Ladi and Bassa (P<0.05). Prevalence odd ratio of FMD was more in extensively managed system relative to intensively managed system, more in adult cattle aged >2 years old. This study has indicated that FMD is an important disease among sedentary cattle in Northern Plateau, however little is currently known about the economic impact of the disease on the local farmers and their livelihoods. As a control measure, efforts should be improved on animal movement during outbreaks while prophylactic control using vaccination should be considered as another option using vaccines containing virus representative of the region.Asian J. Med. Biol. Res. June 2015, 1(2): 169-174

scholarly journals Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus: III. Evaluation of antibodies after infection and vaccination

1987 ◽  
Vol 99 (3) ◽  
pp. 733-744 ◽  
Author(s):  
C. Hamblin ◽  
R. P. Kitching ◽  
A. I. Donaldson ◽  
J. R. Crowther ◽  
I. T. R. Barnett
Keyword(s):  
Disease Virus ◽  
Immunosorbent Assay ◽  
Serum Antibody ◽  
Foot And Mouth Disease ◽  
Immunological Response ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Antibody Levels

SUMMARYInvestigations using a liquid-phase blocking sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies against foot-and-mouth disease virus (FMDV) in sera from sheep and from cattle are reported, and results compared with those obtained by virus neutralization (VN) tests.Serum antibody titres in sheep after primary vaccination and in cattle challenged with a natural aerosol after vaccination were similar by ELISA and VN. However, the antibody levels detected in sera of cattle during early infection and of vaccinated cattle after intradermolingual challenge were clearly greater by ELISA than by VN.The ELISA titres in cattle sera following synthetic peptide vaccination indicated some relationship to protection and were clearly different from those recorded by VN. On the other hand, the antibody levels following conventional vaccination showed that ELISA and VN titres in cattle sera were related to protection. Although there was a good agreement between the ELISA antibody titre and protection for the four vaccines used, by VN the titre which afforded protection varied depending on the vaccine used.The ELISA was considered therefore to be more reliable than the VN and may prove useful for evaluating the immunological response of animals following infection and following vaccination.

scholarly journals Chemiluminescence Immunoassay for the Detection of Antibodies against the 2C and 3ABC Nonstructural Proteins Induced by Infecting Pigs with Foot-and-Mouth Disease Virus

2017 ◽  
Vol 24 (8) ◽  
Author(s):  
Zezhong Liu ◽  
Junjun Shao ◽  
Furong Zhao ◽  
Guangqing Zhou ◽  
Shandian Gao ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Diagnostic Sensitivity ◽  
High Rate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Specificity ◽  
Serum Samples ◽  
Mouth Disease Virus ◽  
Foot And Mouth

ABSTRACT The potential diagnostic value of chemiluminescence immunoassays (CLIAs) has been accepted in recent years, although their use for foot-and-mouth disease (FMD) diagnostics has not been reported. Full-length 3ABC and 2C proteins were expressed in bacteria and purified by affinity chromatography to develop a rapid and accurate approach to distinguish pigs infected with foot-and-mouth disease virus (FMDV) from vaccinated pigs. The recombinant proteins were then used as antigens to develop two CLIAs for the detection of antibodies against nonstructural viral proteins. The diagnostic performance of the two assays was compared by analyzing serum from pigs (naive pigs, n = 63; vaccinated, uninfected pigs, n = 532; naive, infected pigs, n = 117) with a known infection status. The 3ABC-2C CLIA had a higher accuracy rate, with a diagnostic sensitivity of 100% and a diagnostic specificity of 96.5%, than the 3ABC CLIA, which had a diagnostic sensitivity of 95.7% and a diagnostic specificity of 96.0%. The results of the 3ABC-2C CLIA also had a high rate of concordance with those of two commercial FMDV enzyme-linked immunosorbent assay (ELISA) kits used to assess serum collected from 962 pigs in the field (96.2% and 97.8%, respectively). The 3ABC-2C CLIA detected infection in serum samples from infected pigs earlier than the commercial ELISA kits. In addition, the 3ABC-2C CLIA produced results within 15 min. On the basis of these findings, the 3ABC-2C CLIA could serve as the foundation for the development of penside FMD diagnostics and offers an alternative method to detect FMDV infections.

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