scholarly journals Complete Genomic Characterization of Plum bark necrosis stem pitting–associated virus Infecting Sweet Cherry in China

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Jiawei Wang ◽  
Ying Zhai ◽  
Weizhen Liu ◽  
Dongzi Zhu ◽  
Hanu R. Pappu ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Small Rna ◽  
Sweet Cherry ◽  
Rt Pcr ◽  
Rna Deep Sequencing ◽  
Genomic Characterization ◽  
Small Rna Deep Sequencing ◽  
Stem Pitting ◽  
Complete Genomic

Plum bark necrosis stem pitting–associated virus (PBNSPaV) causes the plum bark necrosis stem pitting–associated disease. We obtained the complete genome of a PBNSPaV isolate (PBNSPaV-TA) using small RNA deep sequencing followed by overlapping RT-PCR. To our knowledge, this is the first report of a completed genome of PBNSPaV identified from cherry trees.

scholarly journals Complete Genome Sequence of a Little Cherry Virus-2 Isolate from Sweet Cherry in China

2019 ◽  
Vol 8 (37) ◽  
Author(s):  
Dongzi Zhu ◽  
Jiawei Wang ◽  
Xiaojuan Zong ◽  
Yue Tan ◽  
Hairong Wei ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Sequence ◽  
Small Rna ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Close Relationships ◽  
Sweet Cherry ◽  
Rt Pcr ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

The first complete genome sequence of a little cherry virus-2 (LChV-2-TA) isolate from China was determined using small RNA deep sequencing combined with overlapping reverse transcriptase PCR (RT-PCR). Phylogenetic analysis revealed that LChV-2-TA grouped in a well-supported cluster with members of the genus Ampelovirus with close relationships to previously reported LChV-2 isolates.

Diversity of viruses infecting sweet potato in Beijing based on small RNA deep sequencing and PCR or RT-PCR detection

2020 ◽  
Vol 86 (4) ◽  
pp. 283-289
Author(s):  
Qili Liu ◽  
Yongqiang Li ◽  
Puwen Song ◽  
Feng Zhou ◽  
Yan Qiao ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Deep Sequencing ◽  
Small Rna ◽  
Pcr Detection ◽  
Rt Pcr ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

scholarly journals Whole-Genome Characterization of Prunus necrotic ringspot virus Infecting Sweet Cherry in China

2018 ◽  
Vol 6 (9) ◽  
Author(s):  
Jiawei Wang ◽  
Ying Zhai ◽  
Dongzi Zhu ◽  
Weizhen Liu ◽  
Hanu R. Pappu ◽  
...  
Keyword(s):  
Small Rna ◽  
Sweet Cherry ◽  
Yield Loss ◽  
Ringspot Virus ◽  
Stone Fruits ◽  
Content Type ◽  
Rna Deep Sequencing ◽  
Prunus Necrotic Ringspot Virus ◽  
Small Rna Deep Sequencing

ABSTRACT Prunus necrotic ringspot virus (PNRSV) causes yield loss in most cultivated stone fruits, including sweet cherry. Using a small RNA deep-sequencing approach combined with end-genome sequence cloning, we identified the complete genomes of all three PNRSV strands from PNRSV-infected sweet cherry trees and compared them with those of two previously reported isolates.

scholarly journals Structure and Genome Organization of Cherry Virus A ( Capillovirus, Betaflexiviridae ) from China Using Small RNA Sequencing

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Jiawei Wang ◽  
Ying Zhai ◽  
Weizhen Liu ◽  
Amit Dhingra ◽  
Hanu R. Pappu ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Small Rna ◽  
Genome Organization ◽  
Untranslated Region ◽  
Small Rna Sequencing ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Rna Deep Sequencing ◽  
Rapid Amplification ◽  
Small Rna Deep Sequencing

Cherry virus A (CVA) ( Capillovirus, Betaflexiviridae ) is widely present in cherry-growing areas. We obtained the complete genome of a CVA isolate (CVA-TA) using small RNA deep sequencing, followed by overlapping reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). The newly identified 5′-untranslated region (5′-UTR) from CVA-TA may form additional hairpin and loop structures to stabilize the CVA genome.

Identification of viruses infecting sweet potato in southern China by small RNA deep sequencing and PCR detection

2018 ◽  
Vol 85 (2) ◽  
pp. 122-127 ◽  
Author(s):  
Siqi Ma ◽  
Qiufeng Zheng ◽  
Jiajie Ye ◽  
Wendi Feng ◽  
Guohui Zhou ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Deep Sequencing ◽  
Small Rna ◽  
Southern China ◽  
Pcr Detection ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

Small RNA Deep Sequencing and the Effects of microRNA408 on Root Gravitropic Bending in Arabidopsis

2015 ◽  
Vol 27 (6) ◽  
pp. 495-503 ◽  
Author(s):  
Huasheng Li ◽  
Jinying Lu ◽  
Qiao Sun ◽  
Yu Chen ◽  
Dacheng He ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Small Rna ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing
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