scholarly journals Whole-Genome Characterization of Prunus necrotic ringspot virus Infecting Sweet Cherry in China

2018 ◽  
Vol 6 (9) ◽  
Author(s):  
Jiawei Wang ◽  
Ying Zhai ◽  
Dongzi Zhu ◽  
Weizhen Liu ◽  
Hanu R. Pappu ◽  
...  
Keyword(s):  
Small Rna ◽  
Sweet Cherry ◽  
Yield Loss ◽  
Ringspot Virus ◽  
Stone Fruits ◽  
Content Type ◽  
Rna Deep Sequencing ◽  
Prunus Necrotic Ringspot Virus ◽  
Small Rna Deep Sequencing

ABSTRACT Prunus necrotic ringspot virus (PNRSV) causes yield loss in most cultivated stone fruits, including sweet cherry. Using a small RNA deep-sequencing approach combined with end-genome sequence cloning, we identified the complete genomes of all three PNRSV strands from PNRSV-infected sweet cherry trees and compared them with those of two previously reported isolates.

scholarly journals Complete Genomic Characterization of Plum bark necrosis stem pitting–associated virus Infecting Sweet Cherry in China

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Jiawei Wang ◽  
Ying Zhai ◽  
Weizhen Liu ◽  
Dongzi Zhu ◽  
Hanu R. Pappu ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Small Rna ◽  
Sweet Cherry ◽  
Rt Pcr ◽  
Rna Deep Sequencing ◽  
Genomic Characterization ◽  
Small Rna Deep Sequencing ◽  
Stem Pitting ◽  
Complete Genomic

Plum bark necrosis stem pitting–associated virus (PBNSPaV) causes the plum bark necrosis stem pitting–associated disease. We obtained the complete genome of a PBNSPaV isolate (PBNSPaV-TA) using small RNA deep sequencing followed by overlapping RT-PCR. To our knowledge, this is the first report of a completed genome of PBNSPaV identified from cherry trees.

scholarly journals Complete Genome Sequence of a Little Cherry Virus-2 Isolate from Sweet Cherry in China

2019 ◽  
Vol 8 (37) ◽  
Author(s):  
Dongzi Zhu ◽  
Jiawei Wang ◽  
Xiaojuan Zong ◽  
Yue Tan ◽  
Hairong Wei ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Sequence ◽  
Small Rna ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Close Relationships ◽  
Sweet Cherry ◽  
Rt Pcr ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

The first complete genome sequence of a little cherry virus-2 (LChV-2-TA) isolate from China was determined using small RNA deep sequencing combined with overlapping reverse transcriptase PCR (RT-PCR). Phylogenetic analysis revealed that LChV-2-TA grouped in a well-supported cluster with members of the genus Ampelovirus with close relationships to previously reported LChV-2 isolates.

Complete genome of Hainan papaya ringspot virus using small RNA deep sequencing

Virus Genes ◽  
2014 ◽  
Vol 48 (3) ◽  
pp. 502-508 ◽  
Author(s):  
Yuliang Zhang ◽  
Naitong Yu ◽  
Qixing Huang ◽  
Guohua Yin ◽  
Anping Guo ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Small Rna ◽  
Complete Genome ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

scholarly journals Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

2015 ◽  
Vol 45 (12) ◽  
pp. 2197-2200 ◽  
Author(s):  
Thor Vinícius Martins Fajardo ◽  
Monique Bezerra Nascimento ◽  
Marcelo Eiras ◽  
Osmar Nickel ◽  
Gilvan Pio-Ribeiro
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Causal Agent ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus ◽  
First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

Identification of viruses infecting sweet potato in southern China by small RNA deep sequencing and PCR detection

2018 ◽  
Vol 85 (2) ◽  
pp. 122-127 ◽  
Author(s):  
Siqi Ma ◽  
Qiufeng Zheng ◽  
Jiajie Ye ◽  
Wendi Feng ◽  
Guohui Zhou ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Deep Sequencing ◽  
Small Rna ◽  
Southern China ◽  
Pcr Detection ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing
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