scholarly journals In Vitro Studies on Rickettsia-Host Cell Interactions: Intracellular Growth Cycle of Virulent and Attenuated Rickettsia prowazeki in Chicken Embryo Cells in Slide Chamber Cultures

1975 ◽  
Vol 11 (6) ◽  
pp. 1391-1401 ◽  
Author(s):  
C. L. Wisseman ◽  
A. D. Waddell
Keyword(s):  
Host Cell ◽  
Chicken Embryo ◽  
Growth Cycle ◽  
Cell Interactions ◽  
In Vitro Studies ◽  
Intracellular Growth ◽  
Embryo Cells ◽  
Host Cell Interactions

scholarly journals Identification and Characterization of Cryptosporidium parvum Clec, a Novel C-Type Lectin Domain-Containing Mucin-Like Glycoprotein

2013 ◽  
Vol 81 (9) ◽  
pp. 3356-3365 ◽  
Author(s):  
Seema Bhalchandra ◽  
Jacob Ludington ◽  
Isabelle Coppens ◽  
Honorine D. Ward
Keyword(s):  
Host Cell ◽  
Transmembrane Domain ◽  
Cell Interactions ◽  
Apical Region ◽  
Content Type ◽  
Lectin Domain ◽  
Loop Region ◽  
Wide Range ◽  
Host Cell Interactions

ABSTRACTCryptosporidiumspecies are waterborne apicomplexan parasites that cause diarrheal disease worldwide. Although the mechanisms underlyingCryptosporidium-host cell interactions are not well understood, mucin-like glycoproteins of the parasite are known to mediate attachment and invasionin vitro. We identifiedC. parvumClec (CpClec), a novel mucin-like glycoprotein that contains a C-type lectin domain (CTLD) and has orthologs inC. hominisandC. muris. CTLD-containing proteins are ligand-binding proteins that function in adhesion and signaling and are present in a wide range of organisms, from humans to viruses. However, this is the first report of a CTLD-containing protein in protozoa and inApicomplexa. CpClec is predicted to be a type 1 membrane protein, with a CTLD, an O-glycosylated mucin-like domain, a transmembrane domain, and a cytoplasmic tail containing a YXXϕ sorting motif. The predicted structure ofCpClec displays several characteristics of canonical CTLD-containing proteins, including a long loop region hydrophobic core associated with calcium-dependent glycan binding as well as predicted calcium- and glycan-binding sites.CpClec expression duringC. parvuminfectionin vitrois maximal at 48 h postinfection, suggesting that it is developmentally regulated. The 120-kDa mass of nativeCpClec is greater than predicted, most likely due to O-glycosylation.CpClec is localized to the surface of the apical region and to dense granules of sporozoites and merozoites. Taken together, these findings, along with the known functions ofC. parvummucin-like glycoproteins and of CTLD-containing proteins, strongly implicate a significant role forCpClec inCryptosporidium-host cell interactions.

In Vitro Studies of Rickettsia-Host Cell Interactions: Ultrastructural Study of Rickettsia prowazekii -Infected Chicken Embryo Fibroblasts

1980 ◽  
Vol 29 (2) ◽  
pp. 778-790
Author(s):  
David J. Silverman ◽  
Charles L. Wisseman ◽  
Anna Waddell
Keyword(s):  
Fine Structure ◽  
Host Cell ◽  
Chicken Embryo ◽  
Growth Cycle ◽  
Host Cells ◽  
Slight Reduction ◽  
Intracellular Growth ◽  
Rickettsia Prowazekii ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

Secondary chicken embryo fibroblasts infected in suspension with the Breinl strain of Rickettsia prowazekii and grown in monolayer culture were examined by both transmission and scanning electron microscopy at specific intervals after infection to study the effects of prolonged intracellular growth on the fine structure of the host cell and the rickettsiae. Cytopathological changes in the infected host cells were not apparent until late in the intracellular growth cycle when the cells began to rupture as a result of a large rickettsial burden. The only recognizable changes in heavily infected cells before lysis were the condensation of the intercristal matrix of some mitochondria and the apparent dissociation of ribosomes from the rough-surfaced endoplasmic reticulum. Although the effects of intracellular growth of rickettsiae on the fine structure of the host cell were rather unremarkable when compared with those imposed by Rickettsia rickettsii in a similar cell system, noticeable morphological changes in the rickettsiae were recognized during the intracellular growth cycle. These changes first became apparent about 40 h postinfection and consisted primarily of an increased electron density of the rickettsiae, the appearance of numerous vacuoles in the rickettsial cytoplasm, and a slight reduction in size of the rickettsiae. Changes of this nature may reflect transitional phases of growth characteristically seen in free-living bacterial cell systems.

Interactions between pox viruses

1962 ◽  
Vol 156 (964) ◽  
pp. 388-414 ◽  
Keyword(s):  
Fine Structure ◽  
Host Cell ◽  
Present State ◽  
Cultured Cells ◽  
Growth Cycle ◽  
Cell Interactions ◽  
Considerable Part ◽  
Virus Group ◽  
Host Cell Interactions ◽  
Prototype Virus

In recent years several distinguished virologists who have delivered this lecture have expressed doubts about the validity of including viruses among van Leeuwenhoek’s ‘animalicules’. I share their doubts, but justify my presence here on the grounds that microscopy of a relatively sophisticated type has played a considerable part in the elucidation of several of the problems I will discuss today; and Leeuwenhoek was primarily a microscopist, interested in the fine structure of all natural objects. My predecessors among the animal virologists have ranged over such broad fields as ‘The place of viruses in nature’, ‘Virus-host cell interactions’ and ‘The dynamics of viral functions’. I have chosen a more limited topic, the interactions between pox viruses, and I will preface my account of this with a brief survey of the present state of knowledge of the pox-virus group and the growth cycle of the prototype virus, vaccinia, in cultured cells.

Fluorescent Eimeria bovis sporozoites and meront stages in vitro: a helpful tool to study parasite–host cell interactions

2008 ◽  
Vol 102 (4) ◽  
pp. 777-786 ◽  
Author(s):  
Carlos Hermosilla ◽  
Ivonne Stamm ◽  
Anja Taubert ◽  
Kathleen Lutz ◽  
Horst Zahner ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Interactions ◽  
Helpful Tool ◽  
Host Cell Interactions ◽  
Eimeria Bovis
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