scholarly journals Major outer membrane proteins of Vibrio cholerae and their role in induction of protective immunity through inhibition of intestinal colonization.

1992 ◽  
Vol 60 (11) ◽  
pp. 4848-4855 ◽  
Author(s):  
D K Sengupta ◽  
T K Sengupta ◽  
A C Ghose
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Protective Immunity ◽  
Outer Membrane Proteins ◽  
Intestinal Colonization

scholarly journals Vibrio cholerae tolC Is Required for Bile Resistance and Colonization

2001 ◽  
Vol 69 (7) ◽  
pp. 4681-4685 ◽  
Author(s):  
James E. Bina ◽  
John J. Mekalanos
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Antimicrobial Agents ◽  
Cell Envelope ◽  
Outer Membrane Proteins ◽  
Intestinal Colonization ◽  
Gene Encoding ◽  
Bile Resistance

ABSTRACT TolC and its homologues are outer membrane proteins that are essential for the transport of many molecules across the cell envelope. In this study we characterized the gene encoding Vibrio cholerae TolC. V. cholerae tolC mutants failed to secrete the RTX cytotoxin, were hypersensitive to antimicrobial agents, and were deficient in intestinal colonization.

scholarly journals Characterization of Two Outer Membrane Proteins, FlgO and FlgP, That Influence Vibrio cholerae Motility

2009 ◽  
Vol 191 (18) ◽  
pp. 5669-5679 ◽  
Author(s):  
Raquel M. Martinez ◽  
Madushini N. Dharmasena ◽  
Thomas J. Kirn ◽  
Ronald K. Taylor
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Outer Membrane Proteins ◽  
Sigma 54 ◽  
Colonization Factor ◽  
Motile Cells ◽  
Outer Membrane Lipoprotein ◽  
Important Virulence Factor

ABSTRACT Vibrio cholerae is highly motile by the action of a single polar flagellum. The loss of motility reduces the infectivity of V. cholerae, demonstrating that motility is an important virulence factor. FlrC is the sigma-54-dependent positive regulator of flagellar genes. Recently, the genes VC2206 (flgP) and VC2207 (flgO) were identified as being regulated by FlrC via a microarray analysis of an flrC mutant (D. C. Morris, F. Peng, J. R. Barker, and K. E. Klose, J. Bacteriol. 190:231-239, 2008). FlgP is reported to be an outer membrane lipoprotein required for motility that functions as a colonization factor. The study reported here focuses on the characterization of flgO, the first gene in the flgOP operon. We show that FlgO and FlgP are important for motility, as strains with mutations in the flgOP genes have reduced motility phenotypes. The flgO and flgP mutant populations display fewer motile cells as well as reduced numbers of flagellated cells. The flagella produced by the flgO and flgP mutant strains are shorter in length than the wild-type flagella, which can be restored by inhibiting rotation of the flagellum. FlgO is an outer membrane protein that localizes throughout the membrane and not at the flagellar pole. Although FlgO and FlgP do not specifically localize to the flagellum, they are required for flagellar stability. Due to the nature of these motility defects, we established that the flagellum is not sufficient for adherence; rather, motility is the essential factor required for attachment and thus colonization by V. cholerae O1 of the classical biotype. This study reveals a novel mechanism for which the outer membrane proteins FlgO and FlgP function in motility to mediate flagellar stability and influence attachment and colonization.

scholarly journals Oral Immunization with Cholera Toxin Provides Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model

mBio ◽  
2013 ◽  
Vol 4 (3) ◽  
Author(s):  
M. John Albert ◽  
Abu Salim Mustafa ◽  
Anjum Islam ◽  
Shilpa Haridas
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Cholera Toxin ◽  
Campylobacter Jejuni ◽  
Adult Mouse ◽  
Outer Membrane Proteins ◽  
Cross Reaction ◽  
Intestinal Colonization ◽  
Content Type ◽  
Cholera Vaccines

ABSTRACTImmunity toCampylobacter jejuni, a major diarrheal pathogen, is largely Penner serotype specific. For broad protection, a vaccine should be based on a common antigen(s) present in all strains. In our previous study (M. J. Albert, S. Haridas, D. Steer, G. S. Dhaunsi, A. I. Smith, and B. Adler, Infect. Immun. 75:3070–3073, 2007), we demonstrated that antibody to cholera toxin (CT) cross-reacted with the major outer membrane proteins (MOMPs) of allCampylobacter jejunistrains tested. In the current study, we investigated whether immunization with CT protects against intestinal colonization byC. jejuniin an adult mouse model and whether the nontoxic subunit of CT (CT-B) is the portion mediating cross-reaction. Mice were orally immunized with CT and later challenged withC. jejunistrains (48, 75, and 111) of different serotypes. Control animals were immunized with phosphate-buffered saline. Fecal shedding of challenge organisms was studied daily for 9 days. Serum and fecal antibody responses were studied by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. The cross-reactivity of rabbit CT-B antibody to MOMP was studied by immunoblotting. The reactivity of 21 overlapping 30-mer oligopeptides (based on MOMP’s sequence) against rabbit CT antibody was tested by ELISA. Test animals produced antibodies to CT and MMP in serum and feces and showed resistance to colonization, the vaccine efficacies being 49% (for strain 48), 37% (for strain 75), and 34% (for strain 111) (P, ≤0.05 to ≤0.001). One peptide corresponding to a variable region of MOMP showed significant reactivity. CT-B antibody cross-reacted with MOMP. Since CT-B is a component of oral cholera vaccines, it might be possible to controlC. jejunidiarrhea with these vaccines.IMPORTANCECampylobacter jejuniis a major cause of diarrhea worldwide. Patients who recover fromC. jejunidiarrhea develop immunity to the infecting serotype and remain susceptible to infection with other serotypes. A vaccine based on a common protective antigen(s) present in allC. jejuniserotypes is expected to provide broad protection. In our previous study, we showed that antibody to cholera toxin (CT) reacted with the major outer membrane proteins (MOMPs) from different strains ofC. jejuni. We assumed that the B subunit of the toxin (CT-B), which is nontoxic and a component of licensed oral cholera vaccines, might be the component that cross-reacts with MOMP. In the current study, we showed that orally immunizing mice with CT protected them against colonization upon challenge with different serotypes ofC. jejuni. We also showed that CT-B is the component mediating cross-reaction. Therefore, it might be possible to use cholera vaccines to preventC. jejunidiarrhea. This could result in significant savings in vaccine development and treatment of the disease.

scholarly journals Immune Responses and Protective Immunity in Pangasius Pangasius (Hamilton, 1822) as Induced by Outer Membrane Proteins of Edwardsiella Tarda and Aluminium Hydroxide Adjuvant Complex

2022 ◽  
Author(s):  
Harresh Adikesavalu ◽  
Thangapalam Jawahar Abraham ◽  
Siddhartha Narayan Joardar
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Antibody Production ◽  
Aluminium Hydroxide ◽  
Lymphocyte Proliferation ◽  
Protective Immunity ◽  
Outer Membrane Proteins ◽  
Lethal Dose ◽  
Serum Antibody ◽  
Edwardsiella Tarda

Abstract Edwardsiella tarda is considered one of the important bacterial fish pathogens. The outer membrane proteins (OMPs) of E. tarda are structurally and functionally conserved, and immunogenic. This study assessed the effects of the OMPs of E. tarda CGH9 as a vaccine without aluminium hydroxide [AH] (T1) and with AH adjuvant (T2) on the respiratory burst (ROB) activity, lymphocyte proliferation of head kidney (HK) leukocytes, and serum antibody production in pangas catfish Pangasius pangasius. The ROB activity and lymphocyte proliferation of HK leukocytes increased in both vaccinated groups compared to control. Nonetheless, the T2 group showed a gradual increase in ROB activity and lymphocyte proliferation of HK leukocytes up to 3-weeks post-vaccination (wpv). The serum antibody production in the T1 group decreased initially for up to 2-wpv and increased from 3-wpv; whereas, in the T2 group, the serum-specific antibody levels were significantly high from 1-wpv compared to control. Simultaneously, the protective efficacy in terms of relative percentage survival (RPS) in the T2 group after injecting with a lethal dose of E. tarda CGH9 was high (89.00±15.56) compared to the T1 group (78.00±0.00). Furthermore, the catfish administered with a booster dose of E. tarda OMPs with or without AH adjuvant showed no additional increase in immune response or protective immunity. These results suggested that E. tarda OMPs and AH adjuvant complex has a higher potential to induce protective immunity, which may be a good choice as a vaccine to combat E. tarda infection in catfish.

scholarly journals Evaluation of the human immune response to outer membrane proteins of Vibrio cholerae.

1984 ◽  
Vol 44 (2) ◽  
pp. 439-444 ◽  
Author(s):  
S D Sears ◽  
K Richardson ◽  
C Young ◽  
C D Parker ◽  
M M Levine
Keyword(s):  
Immune Response ◽  
Membrane Proteins ◽  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Outer Membrane Proteins ◽  
Human Immune Response ◽  
Human Immune
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