scholarly journals Cell Division and Deoxyribonucleic Acid Synthesis after a Nutritional Shift-Up of Saccharomyces cerevisiae

1973 ◽  
Vol 114 (2) ◽  
pp. 876-877 ◽  
Author(s):  
G. K. Jacobson ◽  
L. W. Parks
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Division ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Deoxyribonucleic Acid Synthesis

Duration of Availability of Tritiated Thymidine Following Intraperitoneal Injection

1968 ◽  
Vol 3 (1) ◽  
pp. 89-93
Author(s):  
W. K. BLENKINSOPP
Keyword(s):  
Cell Division ◽  
Intraperitoneal Injection ◽  
Deoxyribonucleic Acid ◽  
Direct Evidence ◽  
Tritiated Thymidine ◽  
Indirect Evidence ◽  
Acid Synthesis ◽  
Single Intraperitoneal Injection ◽  
Deoxyribonucleic Acid Synthesis

Much indirect evidence supports the assumption that tritiated thymidine does not label cells which enter the deoxyribonucleic acid synthesis phase (S) more than 1 h after injection. Direct evidence confirming this assumption was obtained by counting labelled epithelial nuclei in mice killed 1, 4 or 6 h after a single intraperitoneal injection of [3H]thymidine; colchicine was used to prevent the increase in number of labelled nuclei which would otherwise have occurred because of cell division. The proportion of cells labelled was the same at 1 h as at 4 or 6 h after injection of [3H]thymidine. Nuclei were regarded as labelled if they were overlaid by 4 grains or more; comparison of nuclear and background labelling indicated that nuclei overlaid by 3 grains or less represented background labelling.

scholarly journals THE TIMING OF DEOXYRIBONUCLEIC ACID SYNTHESIS IN THE CELL CYCLE OF SACCHAROMYCES CEREVISIAE

1965 ◽  
Vol 25 (3) ◽  
pp. 517-528 ◽  
Author(s):  
D. H. Williamson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Dilute Acid ◽  
Deoxyribonucleic Acid Synthesis ◽  
Initial Appearance ◽  
Synchronized Cultures

Randomly dividing cultures of Saccharomyces cerevisiae were briefly exposed to radioactive adenine and then treated successively with dilute acid, ribonuclease, buffered formaldehyde, and NaOH. This treatment was shown to remove virtually all the radioactivity of the labelled cells other than that in DNA. Thus, in subsequent autoradiographs, only cells which had been synthesizing DNA during exposure to the precursor were labelled. The ages of these individuals within the cell cycle were estimated by measuring their sizes. This revealed that incorporation into DNA occurred almost exclusively during the first quarter of the cell cycle, starting with the initial appearance of the bud. This behaviour agreed closely with that of cells growing in artificially synchronized cultures.

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